LOCUS       V030968                 8649 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030968
VERSION     V030968
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4796
                     /label="Erbb2(NM_001003817)"
                     /note="Erbb2(NM_001003817)"
                     /gene="Erbb2"
     CDS             4815..4844
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4863..4886
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4906..4922)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4933..5555
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5704..6292)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6621..6668
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6900..7694)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7729..8086)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(8088..8192)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      8219..8647
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagctggcg gcctggtgcc gttgggggtt cctcctcgcc ctcctgtccc
     1081 ccggagccgc gggtacccaa gtgtgtaccg gtaccgacat gaagttgcga ctccctgcca
     1141 gtcctgagac ccacctggac atgcttcgcc acctctacca gggctgtcag gtggtgcagg
     1201 gcaatttgga gcttacctac ctgcccgcca atgccagcct ctcattcctg caggacatcc
     1261 aggaagtcca gggatacatg ctcatcgctc acaaccgagt gaaacacgtc ccactgcaga
     1321 ggttgcgcat cgtgagaggg actcagctct ttgaggacaa gtatgccctg gctgtgctag
     1381 acaaccgaga ccctttggac aacgtcacca ccgccgcccc aggcagaacc ccagaagggc
     1441 tgcgggagct gcagcttcga agtctcacag agatcttgaa gggaggagtt ttgatccgtg
     1501 ggaaccctca gctctgctac caggacatgg ttttgtggaa ggatgtcctc cgtaagaata
     1561 accagctggc tcctgtcgac atggacacca atcgttcccg ggcctgtcca ccttgtgccc
     1621 caacctgcaa agacaatcac tgttggggtg agagtcctga agactgtcag atcttgactg
     1681 gcaccatctg tactagtggc tgtgcccggt gcaagggccg gctgcccact gactgttgcc
     1741 atgagcagtg tgctgcaggc tgcacgggtc ccaagcattc tgactgcctg gcctgcctcc
     1801 acttcaatca tagtggtatc tgtgagctgc actgcccggc cctcatcacc tacaacacag
     1861 acaccttcga gtccatgctc aaccctgagg gtcgctacac ctttggtgcc agctgtgtga
     1921 ccacctgccc ctacaactac ctctccacgg aagtgggatc ctgcactctg gtctgtcccc
     1981 cgaacaacca agaggtcaca gctgaggacg gaacacagcg gtgtgagaaa tgcagcaagc
     2041 cctgtgctgg agtatgctat ggtctgggca tggagcacct ccgaggggcg agggccatca
     2101 ccagtgacaa tatccaggag tttgctggct gcaagaagat ctttgggagc ctggcatttt
     2161 tgccggagag ctttgatggg aacccctcct ccggcgttgc cccactgaag ccagagcatc
     2221 tccaagtgtt cgaaaccctg gaggagatca caggttacct atacatttca gcatggccag
     2281 agagcttcca agacctcagt gtcttccaga accttcgggt cattcgggga cggattctcc
     2341 atgatggtgc ttactcattg acgttgcaag gcctggggat tcactcactg gggctacgct
     2401 cactgcggga gctgggcagt ggattggctc tcattcaccg caacacccat ctctgctttg
     2461 taaacactgt accttgggac cagctcttcc ggaacccgca ccaggcccta ctccacagtg
     2521 ggaaccggcc agaagaggca tgtggtcttg agggcttggt ctgtaactca ctgtgtgccc
     2581 gtgggcactg ctgggggcca gggcccaccc agtgtgtcaa ctgcagtcag ttcctccggg
     2641 gccaggagtg tgtggaggag tgccgagtat ggaaggggct ccccagggag tatgtgaggg
     2701 gcaagcactg tctgccatgc caccccgagt gtcagcctca aaacagctcg gagacctgct
     2761 atggatcgga ggctgaccag tgtgaggctt gtgcccacta caaggactca tcttcctgtg
     2821 tggctcgctg ccccagtggt gtgaagccag acctctccta catgcctatc tggaagtacc
     2881 cggatgagga gggcatatgt cagccatgcc ccatcaactg cacccactca tgtgtggacc
     2941 tggacgaacg aggctgccca gcagagcaga gagccagccc agtgacattc atcattgcaa
     3001 ctgtggtggg cgtcctgttg ttcctgatca tagtggtggt cattggaatc ctaatcaaac
     3061 gaaggcgaca gaagatccgg aagtatacca tgcgtaggct gctgcaggag accgagctgg
     3121 tggagccgct gacgcccagt ggagctgtgc ccaaccaggc tcagatgcgg atcctaaagg
     3181 agacagagct aaggaagctg aaggtgcttg ggtcaggagc cttcggcact gtctacaagg
     3241 gcatctggat cccagatggg gagaacgtga aaatccccgt ggccatcaag gtgttgaggg
     3301 aaaacacatc tcctaaagct aacaaagaaa tcctagatga agcgtacgtc atggctggtg
     3361 tgggttctcc atatgtgtcc cgcctcctgg gcatctgcct gacatccaca gtgcagctgg
     3421 tgacacagct tatgccctat ggctgccttc tggaccatgt ccgagaacac cgaggtcgct
     3481 taggctccca ggacctgctc aactggtgtg ttcagattgc caaggggatg agctacctgg
     3541 aggaagttcg gcttgttcac agggacctag ctgcccgaaa cgtgctagtc aagagtccca
     3601 accacgtcaa gattaccgac ttcgggctgg cacggctgct ggacattgat gagactgaat
     3661 accatgcaga tgggggcaag gtgcccatca agtggatggc attggaatct attctcagac
     3721 gccggttcac ccatcagagt gatgtgtgga gctatggtgt gactgtgtgg gagctgatga
     3781 cctttggggc caaaccttac gatgggatcc cagctcggga gatccctgat ttgctggaga
     3841 agggagaacg cctacctcag cctccaatct gcaccatcga cgtctacatg atcatggtca
     3901 aatgttggat gattgactcc gaatgtcgcc cgagattccg ggagttggta tcagaattct
     3961 cccgtatggc aagggacccc cagcgctttg tggtcatcca gaacgaggac ttaggcccct
     4021 ccagccccat ggacagcacc ttctaccgtt cactgctgga ggatgatgac atgggggagc
     4081 tggtcgatgc tgaagagtac ctggtacccc agcagggatt cttctcccca gaccctgccc
     4141 taggtactgg gagcacagcc caccgcagac accgcagctc gtcggccagg agtggcggtg
     4201 gtgagctgac actgggcctg gagccctcgg aagaagagcc ccccagatct ccactggctc
     4261 cctccgaagg ggctggctcc gatgtgtttg atggtgacct ggcagtgggg gtaaccaaag
     4321 gactgcagag cctctctcca catgacctca gccctctaca gcggtacagt gaggatccca
     4381 cattacctct gccccccgag actgatggct acgttgctcc cctggcctgc agcccccagc
     4441 ccgagtatgt gaaccagcca gaggttcggc ctcagtctcc cttgacccca gagggtcctc
     4501 cgcctcccat ccgacctgct ggtgctactc tagaaagacc caagactctc tctcctggga
     4561 aaaatggggt tgtcaaagac gtttttgcct ttgggggtgc tgtggagaac cctgaatact
     4621 tagcacccag agcaggcact gcctctcagc cccacccttc tcctgccttc agcccagcct
     4681 ttgacaacct ctattactgg gaccagaact catcggagca gggtcctcca ccaagtacct
     4741 ttgaagggac ccccactgca gagaaccctg agtacctagg cctggatgtg ccagtaacgc
     4801 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc
     4861 tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc
     4921 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     4981 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     5041 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     5101 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     5161 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     5221 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     5281 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     5341 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     5401 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     5461 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     5521 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt
     5581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6421 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac
     6481 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
     6541 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
     6601 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt
     6661 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     6721 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     6781 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     6841 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     6901 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     6961 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     7021 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat
     7081 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     7141 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg
     7201 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     7261 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     7321 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     7381 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     7441 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     7501 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     7561 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     7621 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     7681 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc
     7741 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     7801 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     7861 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     7921 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     7981 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     8041 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt
     8101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8221 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8281 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8341 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8401 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8461 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8521 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8581 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8641 gcgaatttt
//
