LOCUS       V030885                 7812 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030885
VERSION     V030885
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3959
                     /label="Epha2(NM_010139)"
                     /note="Epha2(NM_010139)"
                     /gene="Epha2"
     CDS             3978..4007
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4026..4049
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4069..4085)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4096..4718
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4867..5455)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5784..5831
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6063..6857)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6892..7249)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7251..7355)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7382..7810
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagctccgg gcagtcggtt tctgcctggc gctgctgtgg ggttgcgcgc
     1081 tggcggccgc ggcggcacag ggaaaggaag ttgttttgtt ggacttcgca gcaatgaagg
     1141 gagagctcgg ctggctcacg cacccctatg gcaaagggtg ggacctgatg cagaacatca
     1201 tggacgacat gcctatctac atgtactcgg tgtgcaacgt ggtatccggc gaccaggaca
     1261 actggctccg caccaactgg gtgtaccggg aggaggccga gcgcatcttt attgagctca
     1321 agttcacggt gcgagactgt aacagcttcc cgggtggcgc cagctcatgc aaagagacct
     1381 tcaacctcta ctatgcagag tcagatgtgg actatggcac caacttccag aagcgccagt
     1441 tcaccaagat tgacaccatc gcccctgacg agatcacggt cagcagtgac ttcgaggctc
     1501 gcaatgtcaa gctgaacgta gaggagcgca tggtggggcc ccttacccgg aagggcttct
     1561 acctggcctt ccaggacatc ggcgcctgcg tggcgctgct ctccgttcgc gtctactaca
     1621 agaagtgtcc cgagatgctg cagagcttgg ctcgcttccc cgagaccatt gctgtcgctg
     1681 tctccgatac acaacccctg gccacggtgg ccggtacctg cgtggaccat gccgtggtgc
     1741 cttatggggg cgaggggcct ctcatgcact gcacggtgga tggcgagtgg ctggtgccca
     1801 tcgggcagtg cctgtgccag gaaggctacg agaaggtcga ggatgcctgc cgagcctgtt
     1861 ctccaggatt cttcaagtct gaggcatctg agagcccttg cctggagtgt ccagagcata
     1921 ccctgccatc cacagagggt gccacctcct gccagtgtga agaaggctat ttcagggcac
     1981 ctgaggaccc actgtccatg tcttgcacac gtccaccctc tgcccccaac tacctcacgg
     2041 ccattggcat gggtgccaaa gtagaactgc gttggacagc tcccaaggac actggtggcc
     2101 gccaggacat tgtctacagt gtcacttgcg aacagtgctg gccagagtct ggcgagtgtg
     2161 ggccctgtga ggcgagcgtg cgctattcag aacctcctca cgccctgacc cgcacgagtg
     2221 tgacagtcag tgacctggag ccccacatga actatacctt cgctgtcgaa gcacgcaatg
     2281 gtgtctcagg cctggtgact agccgaagct tccggactgc cagcgtcagt attaaccaaa
     2341 cagagccccc caaagtgagg ctggaggacc gaagcaccac ctccctgagt gtcacctgga
     2401 gcatcccggt gtcacagcag agccgtgtgt ggaagtacga agtcacctac cgcaagaagg
     2461 gggatgccaa cagctataat gtgcgccgca cggaaggctt ctccgtgacc ctggatgacc
     2521 ttgctccgga taccacgtac ctggtgcagg tgcaggcgct gacgcaggag ggccagggag
     2581 ccggcagcaa agtgcacgag ttccagacac tgtccacgga aggatctgcc aacatggcgg
     2641 tgatcggcgg tgtggctgta ggtgttgttt tgcttctggt actggcagga gttggcctct
     2701 tcatccatcg aaggaggagg aacctgcggg ctcgccagtc ctctgaggat gtccgttttt
     2761 ccaagtcaga acaactaaag cccctgaaga cctatgtgga tcctcacact tacgaagacc
     2821 ccaaccaggc tgtactcaag tttaccaccg agatccaccc atcctgtgtg gcaaggcaga
     2881 aggtcattgg agcaggagag tttggagagg tctataaagg gacgctgaag gcatcctcgg
     2941 ggaagaagga gataccggtg gccatcaaga cactgaaagc gggctacact gagaagcagc
     3001 gggtggactt cctgagcgag gccagcatca tgggccagtt tagccaccac aatatcatcc
     3061 gcctggaggg cgtggtctct aaatacaaac ccatgatgat tatcacagag tacatggaga
     3121 atggagcgct agacaagttc cttagggaga aggatggtga gttcagtgta cttcagttgg
     3181 tgggcatgct gaggggtatc gcatccggca tgaagtacct ggccaacatg aactacgtgc
     3241 accgggacct ggccgcccgc aacatcctcg tcaacagcaa cctggtgtgc aaggtgtccg
     3301 attttggcct gtcgcgtgtg ctggaagatg accccgaggc cacctacacc acaagtggcg
     3361 gcaagatccc tattcgatgg acagccccag aggccatttc ctaccgcaag ttcacctcag
     3421 ccagcgatgt gtggagctac ggcattgtca tgtgggaagt gatgacttat ggcgaacggc
     3481 cctactggga actgtcaaac cacgaggtca tgaaagccat caacgacggc ttccggctcc
     3541 ctacgcccat ggactgccct tcagccattt accagctcat gatgcagtgc tggcagcaag
     3601 agcgctcccg ccgacccaag tttgccgaca tcgttagcat cctggacaag ctcatccgag
     3661 cccccgactc cctcaagacg ctggctgact ttgatccccg agtgtccatc cggctgccca
     3721 gcaccagcgg ctcggaggga gtccccttcc gtacggtgtc cgagtggctg gagagcatca
     3781 agatgcaaca gtacacggaa cacttcatgg tggctggcta cacggccatc gagaaggtgg
     3841 tacagatgtc caacgaagac atcaaaagga tcggagtgcg tcttcctggc caccagaagc
     3901 gtattgccta cagcctgctg ggactcaagg accaggtcaa cacagtgggg attcctatca
     3961 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     4021 tcctggatta caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt
     4081 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     4141 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     4201 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4261 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     4321 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4381 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4441 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4501 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4561 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     4621 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     4681 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     4741 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4801 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5281 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5401 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5581 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     5641 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     5701 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     5761 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     5821 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     5881 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     5941 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     6001 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     6061 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     6121 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     6181 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     6241 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     6301 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     6361 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     6421 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     6481 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     6541 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     6601 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     6661 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     6721 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     6781 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     6841 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     6901 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6961 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     7021 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     7081 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     7141 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     7201 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     7261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7321 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7381 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7681 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7741 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7801 aacgcgaatt tt
//
