LOCUS       V018568                 9620 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018568
VERSION     V018568
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             671..4300
                     /EC_number=""
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="precursor of human epidermal growth factor
                     receptor"
                     /protein_id=""
                     /transl_table=1
                     /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF
                     EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN
                     LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI
                     QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG
                     RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN
                     PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG
                     IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV
                     KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS
                     DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW
                     GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG
                     PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE
                     GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP
                     LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA
                     TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI
                     GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKE
                     YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL
                     EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER
                     MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS
                     TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS
                     VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH
                     QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
                     /label="EGFR"
     CDS             4307..4372
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    4396..4984
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5050..5098
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5300..5728
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5742..6071
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6138..6932
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7108..7229
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7278..7294)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7302..7318
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7326..7356)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7371..7392
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7680..8268)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8439..9299)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9300..9404)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgccacc atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc
      721 gctctgcccg gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa
      781 gctcacgcag ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa
      841 ctgtgaggtg gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc
      901 cttcttaaag accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga
      961 gcgaattcct ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta
     1021 tgccttagca gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat
     1081 gagaaattta caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg
     1141 caacgtggag agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc
     1201 gatggacttc cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg
     1261 gagctgctgg ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca
     1321 gcagtgctcc gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc
     1381 tgcaggctgc acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga
     1441 agccacgtgc aaggacacct gccccccact catgctctac aaccccacca cgtaccagat
     1501 ggatgtgaac cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg
     1561 taattatgtg gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga
     1621 gatggaggaa gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg
     1681 taacggaata ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa
     1741 acacttcaaa aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag
     1801 gggtgactcc ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac
     1861 cgtaaaggaa atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct
     1921 ccatgccttt gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc
     1981 tcttgcagtc gtcagcctga acataacatc cttgggatta cgctccctca aggagataag
     2041 tgatggagat gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg
     2101 gaaaaaactg tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa
     2161 cagctgcaag gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg
     2221 cccggagccc agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga
     2281 caagtgcaac cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca
     2341 gtgccaccca gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga
     2401 caactgtatc cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc
     2461 aggagtcatg ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg
     2521 ccacctgtgc catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc
     2581 aacgaatggg cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct
     2641 gctggtggtg gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg
     2701 cacgctgcgg aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga
     2761 agctcccaac caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt
     2821 gctgggctcc ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa
     2881 agttaaaatt cccgtcgcta tcaaggaatt aagagaagca acatctccga aagccaacaa
     2941 ggaaatcctc gatgaagcct acgtgatggc cagcgtggac aacccccacg tgtgccgcct
     3001 gctgggcatc tgcctcacct ccaccgtgca gctcatcacg cagctcatgc ccttcggctg
     3061 cctcctggac tatgtccggg aacacaaaga caatattggc tcccagtacc tgctcaactg
     3121 gtgtgtgcag atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga
     3181 cctggcagcc aggaacgtac tggtgaaaac accgcagcat gtcaagatca cagattttgg
     3241 gcgggccaaa ctgctgggtg cggaagagaa agaataccat gcagaaggag gcaaagtgcc
     3301 tatcaagtgg atggcattgg aatcaatttt acacagaatc tatacccacc agagtgatgt
     3361 ctggagctac ggggtgactg tttgggagtt gatgaccttt ggatccaagc catatgacgg
     3421 aatccctgcc agcgagatct cctccatcct ggagaaagga gaacgcctcc ctcagccacc
     3481 catatgtacc atcgatgtct acatgatcat ggtcaagtgc tggatgatag acgcagatag
     3541 tcgcccaaag ttccgtgagt tgatcatcga attctccaaa atggcccgag acccccagcg
     3601 ctaccttgtc attcaggggg atgaaagaat gcatttgcca agtcctacag actccaactt
     3661 ctaccgtgcc ctgatggatg aagaagacat ggacgacgtg gtggatgccg acgagtacct
     3721 catcccacag cagggcttct tcagcagccc ctccacgtca cggactcccc tcctgagctc
     3781 tctgagtgca accagcaaca attccaccgt ggcttgcatt gatagaaatg ggctgcaaag
     3841 ctgtcccatc aaggaagaca gcttcttgca gcgatacagc tcagacccca caggcgcctt
     3901 gactgaggac agcatagacg acaccttcct cccagtgcct gaatacataa accagtccgt
     3961 tcccaaaagg cccgctggct ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc
     4021 cgcgcccagc agagacccac actaccagga cccccacagc actgcagtgg gcaaccccga
     4081 gtatctcaac actgtccagc ccacctgtgt caacagcaca ttcgacagcc ctgcccactg
     4141 ggcccagaaa ggcagccacc aaattagcct ggacaaccct gactaccagc aggacttctt
     4201 tcccaaggaa gccaagccaa atggcatctt taagggctcc acagctgaaa atgcagaata
     4261 cctaagggtc gcgccacaaa gcagtgaatt tattggagca ctcgaggact acaaagacca
     4321 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagtgagg
     4381 gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4441 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4501 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4561 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4621 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4681 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4741 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4801 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4861 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4921 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4981 ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     5041 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     5101 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5161 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5221 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg
     5281 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5341 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5401 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     5461 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5521 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5581 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5641 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     5701 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5761 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5821 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5881 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5941 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6001 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6061 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6121 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6421 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6481 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6541 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6601 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6661 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6721 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6781 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6841 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6901 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc
     6961 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     7021 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7081 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7201 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7261 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7321 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7381 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7441 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7501 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7561 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7621 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7681 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7741 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7801 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7861 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7921 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7981 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8041 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8101 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8161 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8221 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8281 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8341 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8401 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8461 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8521 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8581 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8641 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8701 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8761 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8821 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8881 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8941 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9001 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9061 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9121 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9181 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9241 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9301 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9361 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9421 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta
     9481 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     9541 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     9601 gcatgaagaa tctgcttagg
//
