LOCUS V018568 9620 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018568 VERSION V018568 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 671..4300 /EC_number="" /codon_start=1 /gene="" /note="" /product="precursor of human epidermal growth factor receptor" /protein_id="" /transl_table=1 /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKE YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" /label="EGFR" CDS 4307..4372 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 4396..4984 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 5050..5098 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5300..5728 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5742..6071 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6138..6932 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7108..7229 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7278..7294) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7302..7318 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7326..7356) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7371..7392 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7680..8268) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8439..9299) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9300..9404) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgccacc atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc 721 gctctgcccg gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa 781 gctcacgcag ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa 841 ctgtgaggtg gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc 901 cttcttaaag accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga 961 gcgaattcct ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta 1021 tgccttagca gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat 1081 gagaaattta caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg 1141 caacgtggag agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc 1201 gatggacttc cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg 1261 gagctgctgg ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca 1321 gcagtgctcc gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc 1381 tgcaggctgc acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga 1441 agccacgtgc aaggacacct gccccccact catgctctac aaccccacca cgtaccagat 1501 ggatgtgaac cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg 1561 taattatgtg gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga 1621 gatggaggaa gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg 1681 taacggaata ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa 1741 acacttcaaa aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag 1801 gggtgactcc ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac 1861 cgtaaaggaa atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct 1921 ccatgccttt gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc 1981 tcttgcagtc gtcagcctga acataacatc cttgggatta cgctccctca aggagataag 2041 tgatggagat gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg 2101 gaaaaaactg tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa 2161 cagctgcaag gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg 2221 cccggagccc agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga 2281 caagtgcaac cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca 2341 gtgccaccca gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga 2401 caactgtatc cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc 2461 aggagtcatg ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg 2521 ccacctgtgc catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc 2581 aacgaatggg cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct 2641 gctggtggtg gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg 2701 cacgctgcgg aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga 2761 agctcccaac caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt 2821 gctgggctcc ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa 2881 agttaaaatt cccgtcgcta tcaaggaatt aagagaagca acatctccga aagccaacaa 2941 ggaaatcctc gatgaagcct acgtgatggc cagcgtggac aacccccacg tgtgccgcct 3001 gctgggcatc tgcctcacct ccaccgtgca gctcatcacg cagctcatgc ccttcggctg 3061 cctcctggac tatgtccggg aacacaaaga caatattggc tcccagtacc tgctcaactg 3121 gtgtgtgcag atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga 3181 cctggcagcc aggaacgtac tggtgaaaac accgcagcat gtcaagatca cagattttgg 3241 gcgggccaaa ctgctgggtg cggaagagaa agaataccat gcagaaggag gcaaagtgcc 3301 tatcaagtgg atggcattgg aatcaatttt acacagaatc tatacccacc agagtgatgt 3361 ctggagctac ggggtgactg tttgggagtt gatgaccttt ggatccaagc catatgacgg 3421 aatccctgcc agcgagatct cctccatcct ggagaaagga gaacgcctcc ctcagccacc 3481 catatgtacc atcgatgtct acatgatcat ggtcaagtgc tggatgatag acgcagatag 3541 tcgcccaaag ttccgtgagt tgatcatcga attctccaaa atggcccgag acccccagcg 3601 ctaccttgtc attcaggggg atgaaagaat gcatttgcca agtcctacag actccaactt 3661 ctaccgtgcc ctgatggatg aagaagacat ggacgacgtg gtggatgccg acgagtacct 3721 catcccacag cagggcttct tcagcagccc ctccacgtca cggactcccc tcctgagctc 3781 tctgagtgca accagcaaca attccaccgt ggcttgcatt gatagaaatg ggctgcaaag 3841 ctgtcccatc aaggaagaca gcttcttgca gcgatacagc tcagacccca caggcgcctt 3901 gactgaggac agcatagacg acaccttcct cccagtgcct gaatacataa accagtccgt 3961 tcccaaaagg cccgctggct ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc 4021 cgcgcccagc agagacccac actaccagga cccccacagc actgcagtgg gcaaccccga 4081 gtatctcaac actgtccagc ccacctgtgt caacagcaca ttcgacagcc ctgcccactg 4141 ggcccagaaa ggcagccacc aaattagcct ggacaaccct gactaccagc aggacttctt 4201 tcccaaggaa gccaagccaa atggcatctt taagggctcc acagctgaaa atgcagaata 4261 cctaagggtc gcgccacaaa gcagtgaatt tattggagca ctcgaggact acaaagacca 4321 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagtgagg 4381 gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 4441 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 4501 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 4561 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 4621 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 4681 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 4741 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 4801 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 4861 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 4921 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 4981 ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 5041 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat 5101 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 5161 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 5221 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg 5281 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 5341 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 5401 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc 5461 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 5521 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 5581 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 5641 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag 5701 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 5761 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 5821 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 5881 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 5941 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 6001 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 6061 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag 6121 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 6181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 6241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 6301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 6361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 6421 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 6481 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 6541 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 6601 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 6661 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 6721 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 6781 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 6841 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 6901 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc 6961 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 7021 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 7081 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 7141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 7201 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 7261 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 7321 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 7381 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 7441 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 7501 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 7561 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 7621 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 7681 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 7741 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 7801 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 7861 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 7921 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 7981 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 8041 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 8101 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 8161 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 8221 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 8281 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 8341 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 8401 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 8461 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 8521 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 8581 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 8641 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 8701 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 8761 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 8821 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 8881 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 8941 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 9001 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 9061 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 9121 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 9181 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 9241 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 9301 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 9361 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 9421 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta 9481 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 9541 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 9601 gcatgaagaa tctgcttagg //