LOCUS       V040798                 7906 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040798
VERSION     V040798
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2562
                     /label="Dtx2(NM_001256096)"
                     /note="Dtx2(NM_001256096)"
                     /gene="Dtx2"
     CDS             2569..2658
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    2682..3270
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3336..3384
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3586..4014
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4028..4357
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4424..5218
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5394..5515
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5564..5580)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5588..5604
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5612..5642)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5657..5678
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5966..6554)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6725..7585)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7586..7690)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc catggcccca
      721 agctcgtctc tgccgcaggt gtaccctagc cacgtggtgg tggctgtgtg ggaatggcag
      781 gatgggcttg gcatttggca cccctacagt gccactgtct gctccttcat tgagcagcat
      841 tttgtccggc agaggggcca gcattttgga ctgggaagcc tggcccacag catcccctta
      901 ggccaagctg acccctcact ggccccttac atcatcgacc tccccagctg gactcagttt
      961 cgccagaaca ctggcaccat gaggtctgtg cgcagacacc tgttctcaca gaattctgcc
     1021 ccaggccagg gcatcgtctg ggagtggctg ggcgatgacg gatcctgggt agcctatgaa
     1081 gctagaatct gtgactatct ggaacagcaa gtggcccggg gcatccaggt cgtggacttg
     1141 gcaccactgg ggtataacta tactgtcaac tatgccaccc taacccaaac caacaagact
     1201 tccagcttct gccggagtgt gcgacgccaa gtggggccag tttacccagt gacttcagac
     1261 atcgctgttc cacgccaaat gggacttatc tgcttttgcc aacagtgcct ccatggtagc
     1321 ggaactggcc ctgtgtcggg ccgctaccgc cactccatga ccaacctgcc tgcatatcct
     1381 gccccccaag caccccaccg gaccaccact gtctctgggg cccaccaggc ctttgcccca
     1441 tacaataaac cttcactgtc tggggccaga tctgcaccaa ggttgaacac caccaacccc
     1501 tgggctgcag cacctcctgt tgcaggcaac cagtccctgt tccactccag cctctcccac
     1561 ctggggcctc agctcctgcc ctcaggaccg tccacctcca gtggagccag tgcctccttc
     1621 cccagcgggc cctcctccag cagcccaggg agcgccccca ccactgtgcc cgtgcagatg
     1681 ccaaaggcca gcagggtcca gcaggcgctt gcaggcatga cgagtgtgct gtcagccatt
     1741 ggactccctg tgtgtcttag ccgtgcaccc cggcccaccg gccctcccgc ctcccgtccg
     1801 gcctctaaaa gtcacagctc agttaagagg ctgaggaaaa tgtccgtaaa agaaggggcg
     1861 cctaagcccg agccggagca ggtgatcaga aagtacaccg aggagctgaa agtggcccct
     1921 gaagaggact gcattatctg tatggagaaa ctggctgtgg cttctgggta cagtgacatg
     1981 actgacagca aggccctcgg gcccatggtc gtgggccgcc tcaccaagtg cagccatgcc
     2041 ttccacctgc tgtgcctgct ggccatgtat tgcaacggga acaaggacgg gagtcttcag
     2101 tgtccgtcgt gcaaaaccat ctatggggag aagactggga cgcagccctg ggggaagatg
     2161 gaggtgttca ggttccaaat gtccctccca ggccatgaag actgtgggac tatactcatt
     2221 gtttacaaca tccctcatgg catccaggga ccagagcacc ccagccctgg gaagccattc
     2281 actgcccggg gctttccccg gcaatgctac ctgccagata gccctcaggg ccgcaaggtc
     2341 ctggagctcc tgaaggtggc ctggaagagg cgactcattt ttaccgtggg cacttccagc
     2401 accacaggtg agaccgacac cgtggtgtgg aacgagatcc accacaagac cgagatggac
     2461 cgcaacgtga caggccacgg ctatccggac cccaactacc tgcagaatgt gctggctgag
     2521 ctcgctgccc agggagtgac cgaagactgc ctggaacagc agctcgagga acaaaaacta
     2581 atatcagagg aagatttgga acagaaacta atcagtgaag aagatttaga gcaaaagtta
     2641 atttccgagg aggacttata gtgagggccc gatatctcga caatcaacct ctggattaca
     2701 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2761 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2821 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2881 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2941 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3001 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3061 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     3121 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3181 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3241 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg
     3301 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     3361 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     3421 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     3481 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     3541 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3601 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3661 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3721 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
     3781 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3841 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3901 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
     3961 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4021 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4081 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4141 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4201 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4261 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4321 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4381 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4441 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4501 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4561 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     4621 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4681 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     4741 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     4801 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4861 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4921 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     4981 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5041 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5101 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5161 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5221 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5281 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5341 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5401 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5461 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5521 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     5581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5641 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5701 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5761 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     6181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     6421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6961 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7021 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7081 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7141 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7201 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7261 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7321 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7381 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7441 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7501 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7561 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7621 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7681 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     7741 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7801 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     7861 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg
//
