LOCUS       V030045                 6213 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030045
VERSION     V030045
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2360
                     /label="Dok3(NM_013739)"
                     /note="Dok3(NM_013739)"
                     /gene="Dok3"
     CDS             2379..2408
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2427..2450
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2470..2486)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2497..3119
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3268..3856)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4185..4232
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4464..5258)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5293..5650)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5652..5756)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5783..6211
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagtctgtg gagcccccgg tcaaagacgg catcctctac cagcagcacg
     1081 taaagtttgg caagaaatgc tggcgcaaag tgtgggctct gctgtatgcg ggaggcccat
     1141 caggggtagc tcggctagaa agctgggacg tgcgtgatgg tggcctggga ccagcaggcg
     1201 acaggtccac agggcccagc cgtcgagggg aacgccgggt catacgcttg gctgactgtg
     1261 tatctgtcct gcctgcggat ggcgagagct gtcccaggga cactggtgcc ttcctgatta
     1321 ccaccactga gcgaagccac ctgttggctg cacagcaccg ccagtcctgg gtggacccca
     1381 tctgtcagct ggccttcccg ggtaccggag aatgttcgtc aggatcagga caggctgaga
     1441 gtccaaaaag gggctttgtt cccatggagg aaaactctat ctactcctcc tggcaggaag
     1501 tgaccgagtt tccggtgatc gtgcagagga cagaggccac ctcccgctgc cagctgaaag
     1561 gaccctacct cctggtgctg ggccaagatg acatccaact gagggagaca tccaagcccc
     1621 aggcctgttt tagctggccc taccgtttcc tgcgcaagta cggctctgac aagggtgtgt
     1681 tctcgtttga ggctggccgc cgctgtgact caggtgaggg cctttttgcc ttcagtagcc
     1741 cgcgtgcccc agacatatgt ggggttgtgg ctgccgccat tgcccgccag cgggagcgtc
     1801 ttccagagct ggccatgtcc ccaccctgcc ccctgcctcg ggccctctcc ctgccctccc
     1861 tagagccccc tggagagctt cgggaggtgg ccccaggatt tgagctgccc actcccagaa
     1921 agctgcctct aactgatccc gggcctcaaa gcctaccact gctgctcagc cccacacaag
     1981 aaggaccggc atccggtctc tatgcgtccg tgtgcaagca gaccagcaag cacacaggca
     2041 cggcggagca tctctatgag aacgtgtgca tgctggaggc cagccctggg ctgaccaatg
     2101 ggggtcctga agcccaagag ggcccccctg gtggccgcag ccccctgggc agccctatct
     2161 accataacac tgaggatctg agttggccgg gctcggccca ggacagcaat ctggaagccc
     2221 agtaccggag gctgctggaa ctggagctgg atgaggccgg aagcgccggc cgctctggag
     2281 cgcaggcagg catcaaggcc aagctggtga ccctgctgac ccgtgaacgg aagaagggcc
     2341 ccgccccctg tgaccggccc acgcgtacgc ggccgctcga gcagaaactc atctcagaag
     2401 aggatctggc agcaaatgat atcctggatt acaaggatga cgacgataag gtttaaacgg
     2461 ccggccgcgg tcatagctgt ttcctgaaca gatcccgggt ggcatccctg tgacccctcc
     2521 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     2581 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     2641 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     2701 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     2761 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     2821 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     2881 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     2941 caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag
     3001 gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt
     3061 tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg
     3121 ggtacgcggc cagcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3241 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
     4021 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     4081 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     4141 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     4201 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
     4261 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
     4321 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     4381 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     4441 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     4501 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     4561 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg atccgccaca
     4621 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     4681 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     4741 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     4801 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     4861 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     4921 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     4981 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     5041 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     5101 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     5161 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     5221 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     5281 tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     5341 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     5401 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     5461 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     5521 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     5581 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt
     5641 tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5761 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5821 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6061 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6121 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6181 gagctgattt aacaaaaatt taacgcgaat ttt
//
