LOCUS V031313 8187 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031313 VERSION V031313 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..4334 /label="Diaph2(NM_172493)" /note="Diaph2(NM_172493)" /gene="Diaph2" CDS 4353..4382 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 4401..4424 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(4444..4460) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 4471..5093 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(5242..5830) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 6159..6206 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(6438..7232) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(7267..7624) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7626..7730) /gene="bla" /label="AmpR promoter" rep_origin 7757..8185 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggaggagctc ggggcggcgg cgtcgggagc cggaggcggc ggcggtggcg 1081 gcgaggaaca cggcgggggc cggagcaaca agcgaggcgc ggggaaccgg gccgccaacg 1141 aagaggagac gagaaacaaa cccaaactga acattcaaat aaaaactttg gcagatgatg 1201 tgcgtgaccg aattacaagt ttcagaaaat ctgccaccaa gagagaaaag cctgttattc 1261 aacattctat tgattatcaa actgcagtgg ttgagattcc cccagctctg atagtacatg 1321 atgaccgatc tttgattttg tctgaaaagg aagttctgga cctctttgag aaaatgatgg 1381 aagatatgaa ccttaatgaa gaaaagaaag ctcctttgcg aaagaaggat tttagcatca 1441 agcgtgagat ggttgtccag tatatttctg ctacttccaa atctggtggc ctgaaaaaca 1501 gcaaacatga gttcaccctt tcttcacaag aatatgttca tgaattacga tctggcatct 1561 cagatgagaa acttctcaac tgcctggaat ctttgagagt gtctttaacc agccatcctg 1621 tcagctgggt caacaacttt ggctatgaag gacttggagt cttactggat gtgctggaga 1681 agcttctgga caaaaaacag caagaaaata tcgacaagaa gaatcagtac aaagtcattc 1741 agtgcctgaa agcatttatg aataataagt ttggactaca aagaattcta ggagacgaga 1801 gaagcctttt actgttggca agagcaattg acccgaaaca acagaatatg atgactgaaa 1861 tagtaaaaat cctttctgct atttgcattg ttggggaaga aaacattcta gacaaacttt 1921 tagggggcat cactgctgca gctgaactaa ataatagaga acgattttca ccaattgtgg 1981 aaggcttaga aaataatgaa gccttgcatc tccaggtggc ctgcatgcag ttcataaatg 2041 cacttgtcac ctctccatat gatcttgatt ttcgaattca tttgaggaat gaattccttc 2101 gttgtggatt aaaagcaatg ctccctactc ttaaagagat agagaatgaa gggcttgata 2161 ttcagttgag agtgtttgaa gagaacaaag aagatgacct aagtgaatta tcccaccgtc 2221 tcaatgacat tcgagctgaa atggatgata ttaatgaagt ctaccatctg ctgtataata 2281 tgctgaagga cactgctgcg gaaccttact tattatccat tctccaacac tttctgctca 2341 tcagaaatga ttactacatc aggccacagt attataaaat cattgaggag tgtgtttcac 2401 agatagtgct acactgcagt ggcatggacc cagacttcaa gtaccggcag cgcatagact 2461 ttgatttcac tcatctccta gatgcttgtg taaataaggc aaaagttgaa gaaaatgagc 2521 aaaaagcaat ggaattttca aagaagttcg atgaagaatt cacagctcgt caggaagctc 2581 aagcagagct tcagaaaaga gatgaaaaaa tcaaagaact ggaaacagaa atccagcagc 2641 ttcgaggaca gggagttcca tctgcaattc caggtccacc tccaccacct ccactgccag 2701 gtgcaggacc atgcccacca ccaccacctc ctcctcctcc accacctcca cttccaggag 2761 tcgtccctcc tcctcctcca ccattacctg ggatgcctgg tattccacca ccacctccac 2821 cacctttgtc tggagtacct cctccaccac caccccctgg gggagttttt cctctcctat 2881 caggaccaat tgagctacca tatggaatga agcagaaaaa attatataag cctgacatac 2941 ctatgaaaag aatcaactgg tccaagattg agcctaaaga attatccgaa aactgtgttt 3001 ggctaaaact taaagaagag aagtatgaga atgcagatct atttgcaaaa ctggccttga 3061 catttccttc ccagatgaaa ggtcaaagga atacagaggc tgcagaagaa aataggagtg 3121 ggcctccaaa gaagaaagtg aaggaattga gaattttgga taccaaaaca gcccagaatc 3181 tatctatctt ccttggatct taccgcatgc catacgagga aataaagaac attattttgg 3241 aggttaatga agagatgctc agtgaggccc taatccagaa tcttgtaaaa tatcttcctg 3301 accagaacgc actccgagaa ctagcccagc ttaagagtga atatgatgat ctctgtgagc 3361 ctgagcagtt tggtgttgtg atgagcacgg tgaaaatgtt acgtcctcgt ctcacgagca 3421 tccttttcaa actcaccttt gaagaacatg tcaacaacat caaaccaagc atcatagcag 3481 taactcttgc atgtgaagaa ctgaagaaaa gtgaaagctt taagagactc ttagagttga 3541 ttcttctggt tggaaactac atgaactcag gctccaggaa tgcccagtct ctaggtttta 3601 agattaactt tctgtgtaag atcaaagaca caaaatcagc agatcaaaaa tccacccttt 3661 tgcattttct tgctgaaatc tgtgatgaga aataccggga tatcctgaaa tttcctgatg 3721 agctggagca tgtggaaagc gcaggcaaag tttcagctca aatactcaag agcaaccttg 3781 tagcaatgga acaaagcatt cttcatctgg agaaaaacat caagaatttc ccaccagcag 3841 aatcgcacca tgataagttt gtggaaaaga tgatgagctt tacccagaat gccagagaac 3901 agtatgacaa actgtccacc atgcacagca atatgttgaa gctctatgag agtctgggag 3961 aatacttcat ttttgacccg aacacagtaa acatggaaga attttttggt gatctcaaca 4021 ccttcagaac cctatttttg gaagcactga aagaaaacca caagagaaag gaaatggaag 4081 agaagagcag gagagcaaag cttgcaaaag agaaagcgga acaagagaag ctggaacgcc 4141 agaagaagaa gaagcaactc attgacataa acaaagaggg tgatgagacg ggagtgatgg 4201 ataatcttct ggaagcccta cagtcaggtg cagcattcag agaccgcaga aagcgaattc 4261 caagaaatcc agataacaga cggccccctc tggaaagatc tcgctctcgc cacaatggag 4321 ccatgtcatc caagacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 4381 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 4441 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 4501 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 4561 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 4621 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 4681 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 4741 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 4801 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 4861 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 4921 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 4981 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 5041 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 5101 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5161 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5221 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5281 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5341 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5401 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5461 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5521 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5581 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5641 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5701 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5761 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5821 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5881 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5941 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 6001 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 6061 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 6121 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 6181 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 6241 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 6301 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 6361 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 6421 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 6481 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 6541 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 6601 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 6661 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 6721 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 6781 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 6841 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 6901 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 6961 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 7021 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 7081 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 7141 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 7201 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 7261 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 7321 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 7381 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 7441 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 7501 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 7561 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 7621 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7681 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7741 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 7801 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 7861 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 7921 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 7981 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 8041 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 8101 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 8161 atttaacaaa aatttaacgc gaatttt //