LOCUS       V029539                 6726 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029539
VERSION     V029539
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2873
                     /label="Ddx5(NM_007840)"
                     /note="Ddx5(NM_007840)"
                     /gene="Ddx5"
     CDS             2892..2921
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2940..2963
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2983..2999)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3010..3632
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3781..4369)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4698..4745
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4977..5771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5806..6163)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6165..6269)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6296..6724
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtcgagttat tctagtgacc gagaccgcgg ccgggatcga gggtttggtg
     1081 cacctcgatt tggagggagt agaacaggac ccctctctgg aaagaagttt ggaaatcctg
     1141 gggagaaact agttaaaaag aagtggaatc ttgatgagct gcccaaattt gagaagaatt
     1201 tttatcaaga acaccctgat ttggcaaggc gcaccgcaca agaggtagat acatacagaa
     1261 gaagcaagga aattacagtt agaggtcaca actgtccaaa acctgttctg aatttttatg
     1321 aagcaaactt tcctgcgaat gtcatggatg tgattgcaag gcagaacttt actgaaccca
     1381 ctgctattca agctcagggc tggccagttg ctctcagtgg attggatatg gttggagtag
     1441 ctcagactgg atctgggaaa acattatctt atttgctgcc tgccattgta cacataaacc
     1501 accagccatt cctagagaga ggtgatgggc ctatttgctt ggtgctggca ccaactcgag
     1561 aactggcaca gcaggtgcag caagtggctg ctgaatattg tcgagcttgt cgcttgaagt
     1621 ctacttgcat ctatggtggt gctcccaaag gaccacagat tcgtgatttg gaaagaggtg
     1681 tggaaatctg tattgcaaca cctggaagac tgattgactt tttagagtgt gggaaaacca
     1741 atctgagaag aacaacttac cttgtccttg atgaagctga taggatgctt gatatgggat
     1801 ttgaacccca gataaggaaa attgtggatc aaataagacc tgataggcaa acactaatgt
     1861 ggagtgcaac ttggccaaaa gaagtaagac agcttgctga agatttcctg aaagactata
     1921 ttcatatcaa tattggtgca ctggaactga gtgcaaacca taacattctt cagattgtgg
     1981 atgtatgtca tgatgtcgaa aaggatgaaa agcttattcg tctgatggaa gaaatcatga
     2041 gtgagaagga gaataaaact attgtttttg ttgaaaccaa aagaagatgt gatgaactta
     2101 ccagaaaaat gaggagagat gggtggcctg ccatgggcat ccatggtgac aagagtcagc
     2161 aggaacgtga ctgggttcta aatgaattca aacatggaaa agctcctatt ctgattgcta
     2221 ccgatgtggc ctccagaggg ctagatgtgg aagatgtgaa atttgtcatc aattatgact
     2281 accctaactc ctcagaggat tatattcatc gaattggaag aactgctcgc agtaccaaaa
     2341 caggcacagc atacactttc tttacaccta ataacataaa gcaagtgagc gaccttatct
     2401 ctgtgcttcg ggaagctaat caagcaatta atcccaagtt gcttcagttg gtcgaagaca
     2461 gaggttcagg tcgttccagg ggtagaggag gcatgaagga cgatcgtcgt gacagatact
     2521 ctgcaggcaa aaggggtgga tttaatacct ttagagacag ggaaaactat gacagaggct
     2581 actctaatct gcttaagaga gattttgggg ctaaaactca gaatggtgtt tacagtgctg
     2641 caaattacac caatgggagc tttggaagta attttgtatc tgctggcata cagaccagtt
     2701 ttaggactgg taatccaaca gggacttacc agaacggtta tgatagcact cagcaatatg
     2761 gaagtaatgt tgcaaatatg cacaatggta tgaaccaaca ggcatatgca tatcctgcta
     2821 ccgcagctgc tgcgcctatg attggctatc ccatgccaac agggtattct caaacgcgta
     2881 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     2941 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     3001 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     3061 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     3121 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     3181 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     3241 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     3301 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     3361 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     3421 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     3481 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     3541 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     3601 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     3661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4501 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     4561 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     4621 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     4681 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     4741 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     4801 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     4861 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     4921 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     4981 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5041 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     5101 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     5161 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     5221 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     5281 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     5341 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     5401 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     5461 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     5521 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     5581 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     5641 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     5701 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     5761 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     5821 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     5881 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     5941 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     6001 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     6061 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6121 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     6181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     6301 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6361 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6421 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6481 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6541 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6601 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6661 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6721 aatttt
//
