LOCUS       V031129                 6510 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031129
VERSION     V031129
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1038..2657
                     /label="Ddx28(NM_028038)"
                     /note="Ddx28(NM_028038)"
                     /gene="Ddx28"
     CDS             2676..2705
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2724..2747
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2767..2783)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2794..3416
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3565..4153)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4482..4529
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4761..5555)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5590..5947)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5949..6053)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6080..6508
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccgg cgcgcccatg gctttagccg ggccatcgcg gctgctggcg ttagcagtac
     1081 ggttgcttct ggagcctcga cggaacctag tggtgcgcgg ttccgaccag tccttgccag
     1141 tggtgcgcgt tccgcgggct ctgcagcgac ggcaggaaca gcgacagagc ggccgtggga
     1201 gtttgcagcg gccagtgcta gtgcgccccg gtcccctgct ggtctcggcg cggcggccgg
     1261 agttgaacca gcccgcacgc ctcacgctgg gccgttggga gcgagcgcca ctggcttccc
     1321 gtggctggaa gcatcggcga tcgcggcaag accatttctc catagagcga gtgcagcaag
     1381 aggcgcccgc cctgaggaac ctctcgtccc ggggcagctt cgtggacctg ggtctggaac
     1441 cccgagtgct gctggcgctt caggaggctg tccccgaagt tgtgcagcca acctcagtgc
     1501 agtcgaaaac catcccccca ctgcttcgag gccgccacct cctttgcgct gcggaaactg
     1561 gcagtggcaa gacgcttagc tacctactgc ccctatttca gcggctcctg agagggtcag
     1621 acctggactc ccgcagtttc acggctcctc gaggcttggt tctggtacct tcccgagaac
     1681 tagccgaaca ggtgcaggca gtggcccagt cactgggtgg gtacttgggc ctgcaggtga
     1741 tagaacttgg cggaggcctc gggatgagta ggctcaaact tcagctgtat agacgaccgg
     1801 cagccgatgt gttagtggct actccagggg cgctgtggaa ggctctgaaa agtcaactga
     1861 tcagcttaca gcatcttaac ttcatagtgc tagatgaagt agacacactg ctagatgaaa
     1921 gcttcctgga actggtggac tacatcttgg aaaagagccc tatagcagaa agtccagccg
     1981 agttagaaga cccctttaat cccaaagccc agttagtgct ggtgggagcc acgtttccag
     2041 aaggcctaaa ccagttactg agtaaagtca ccagtccaga cagtctgacc accattacca
     2101 gctccaagct gcactgtctc atgcctcatg tcagacagac atttatgaga ctgaagggag
     2161 cagacaaggt gacagaactg gttcagatcc tcaaacagca ggacaaagca agtaagacgg
     2221 aaccctcggg aactgtcctg gtattctgca acagcgccag caccgtgaac tggcttgggt
     2281 acattctgga tgaccacaag attcaacatc taaggctgca agggcaaatg ccggcctcaa
     2341 tgagggcagg tatcttccag agtttccaga agggctccca gaacatactt gtctgcacag
     2401 acatcgcctc ccgaggccta gacagtgtcc acgtggaagt ggttatcaat tacgatttcc
     2461 cccctaccct gcaggattac atccacagag caggaagggt gggtcgtgtg ggcagcgaag
     2521 tgccagggtc cgttatcagt tttgtgaccc acccctggga tgtgagcctg gttcagaaaa
     2581 ttgaactggc agctcgccga aggagaagcc ttccaggact agcatcttca gtgggggacc
     2641 ctttgcctca aaaggccacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     2701 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     2761 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     2821 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     2881 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     2941 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     3001 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     3061 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     3121 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     3181 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     3241 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     3301 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     3361 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     3421 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3481 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4141 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4201 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4261 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     4321 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     4381 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     4441 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     4501 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     4561 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     4621 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     4681 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     4741 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     4801 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     4861 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     4921 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     4981 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     5041 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     5101 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     5161 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     5221 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     5281 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     5341 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     5401 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     5461 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     5521 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     5581 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     5641 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     5701 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     5761 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     5821 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5881 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     5941 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6061 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6121 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6181 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6241 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6301 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6361 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6421 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6481 ctgatttaac aaaaatttaa cgcgaatttt
//
