LOCUS       V030994                 6321 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030994
VERSION     V030994
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2468
                     /label="Ddc(NM_001417457)"
                     /note="Ddc(NM_001417457)"
                     /gene="Ddc"
     CDS             2487..2516
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2535..2558
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2578..2594)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2605..3227
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3376..3964)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4293..4340
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4572..5366)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5401..5758)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5760..5864)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5891..6319
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggattcccgt gaattccgga ggagaggcaa ggagatggtg gattatatag
     1081 ctgactatct ggatggcatt gagggtcgtc cagtgtaccc tgatgtggag cctggctatc
     1141 ttcggcccct gatccctgcc actgcccccc aggagccaga aacatacgag gacataatca
     1201 aagacatcga gaagataatc atgccagggg tgacacactg gcacagtccc tatttcttcg
     1261 cttacttccc cacggctagc tcatacccag ctatgcttgc agacatgctg tgtggtgcta
     1321 ttggctgcat tggtttctcc tgggctgcaa gcccagcgtg cacagagctg gagaccgtga
     1381 tgatggactg gctggggaag atgctggagc tgccagaggc ctttttggct ggaagagctg
     1441 gggaaggggg aggagtgatc cagggaagtg ccagtgaagc caccttggtg gccctactgg
     1501 ctgctcggac taaagttatc cgccagctgc aggcagcctc cccagagttc acacaagctg
     1561 ctatcatgga aaagctggtt gcttacacat ctgatcaggc gcattcctct gtagaaagag
     1621 ctgggttaat tggtggaata aagctaaaag cagtcccttc ggatggcaac ttttccatga
     1681 gagcttctgc ccttcgggaa gccctggagc gggacaaggc agctggcctg attccattct
     1741 ttgtggtcgc tacactgggg accacatcct gctgttcttt tgacaatctc ctggaagtgg
     1801 gtcccatctg caaccaggag ggtgtgtggc tgcacattga cgctgcttac gcgggcagtg
     1861 cctttatctg tcctgaattc cggtatcttc tgaatggtgt ggagtttgca gattccttta
     1921 actttaatcc ccacaagtgg cttttggtga actttgactg ctctgccatg tgggtgaaga
     1981 ggaggactga cttaaccgga gcctttaata tggaccctgt ttatctaaag cacagtcacc
     2041 aggactcagg attcatcact gactacaggc actggcagat cccactgggg cgacgatttc
     2101 gctctttgaa aatgtggttt gtttttagaa tgtacggagt caaggggctg caggcttaca
     2161 tccgaaagca cgtggagctg tctcatgagt ttgagtcact ggtacgccag gaccctcgct
     2221 ttgaaatttg cacagaagtc attcttgggt tggtctgctt ccggctaaag ggctccaatg
     2281 agttgaacga aactctctta caaagaataa acagcgccaa aaaaatccac ttggttccat
     2341 gtcgtctccg agacaagttt gtgctacgct ttgctgtgtg cgctcgcact gtggagtctg
     2401 cccacgtgca gctggcctgg gaacacatca gtgatctagc aagcagtgtg ctgagggcag
     2461 agaaagaaac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag
     2521 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc
     2581 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     2641 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     2701 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     2761 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     2821 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     2881 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     2941 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     3001 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     3061 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     3121 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     3181 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     3241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     4141 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     4201 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     4261 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     4321 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     4381 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     4441 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     4501 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     4561 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     4621 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     4681 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca
     4741 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     4801 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     4861 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     4921 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     4981 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     5041 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     5101 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     5161 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     5221 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     5281 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     5341 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     5401 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     5461 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     5521 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     5581 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     5641 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     5701 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     5761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5881 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6301 caaaaattta acgcgaattt t
//
