LOCUS V030994 6321 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030994 VERSION V030994 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2468 /label="Ddc(NM_001417457)" /note="Ddc(NM_001417457)" /gene="Ddc" CDS 2487..2516 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2535..2558 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2578..2594) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2605..3227 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3376..3964) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4293..4340 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4572..5366) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5401..5758) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5760..5864) /gene="bla" /label="AmpR promoter" rep_origin 5891..6319 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggattcccgt gaattccgga ggagaggcaa ggagatggtg gattatatag 1081 ctgactatct ggatggcatt gagggtcgtc cagtgtaccc tgatgtggag cctggctatc 1141 ttcggcccct gatccctgcc actgcccccc aggagccaga aacatacgag gacataatca 1201 aagacatcga gaagataatc atgccagggg tgacacactg gcacagtccc tatttcttcg 1261 cttacttccc cacggctagc tcatacccag ctatgcttgc agacatgctg tgtggtgcta 1321 ttggctgcat tggtttctcc tgggctgcaa gcccagcgtg cacagagctg gagaccgtga 1381 tgatggactg gctggggaag atgctggagc tgccagaggc ctttttggct ggaagagctg 1441 gggaaggggg aggagtgatc cagggaagtg ccagtgaagc caccttggtg gccctactgg 1501 ctgctcggac taaagttatc cgccagctgc aggcagcctc cccagagttc acacaagctg 1561 ctatcatgga aaagctggtt gcttacacat ctgatcaggc gcattcctct gtagaaagag 1621 ctgggttaat tggtggaata aagctaaaag cagtcccttc ggatggcaac ttttccatga 1681 gagcttctgc ccttcgggaa gccctggagc gggacaaggc agctggcctg attccattct 1741 ttgtggtcgc tacactgggg accacatcct gctgttcttt tgacaatctc ctggaagtgg 1801 gtcccatctg caaccaggag ggtgtgtggc tgcacattga cgctgcttac gcgggcagtg 1861 cctttatctg tcctgaattc cggtatcttc tgaatggtgt ggagtttgca gattccttta 1921 actttaatcc ccacaagtgg cttttggtga actttgactg ctctgccatg tgggtgaaga 1981 ggaggactga cttaaccgga gcctttaata tggaccctgt ttatctaaag cacagtcacc 2041 aggactcagg attcatcact gactacaggc actggcagat cccactgggg cgacgatttc 2101 gctctttgaa aatgtggttt gtttttagaa tgtacggagt caaggggctg caggcttaca 2161 tccgaaagca cgtggagctg tctcatgagt ttgagtcact ggtacgccag gaccctcgct 2221 ttgaaatttg cacagaagtc attcttgggt tggtctgctt ccggctaaag ggctccaatg 2281 agttgaacga aactctctta caaagaataa acagcgccaa aaaaatccac ttggttccat 2341 gtcgtctccg agacaagttt gtgctacgct ttgctgtgtg cgctcgcact gtggagtctg 2401 cccacgtgca gctggcctgg gaacacatca gtgatctagc aagcagtgtg ctgagggcag 2461 agaaagaaac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag 2521 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc 2581 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc 2641 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc 2701 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg 2761 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc 2821 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt 2881 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa 2941 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc 3001 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc 3061 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag 3121 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc 3181 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca 3241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc 4141 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg 4201 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga 4261 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt 4321 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt 4381 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag 4441 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc 4501 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc 4561 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg 4621 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca 4681 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca 4741 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg 4801 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt 4861 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct 4921 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta 4981 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca 5041 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc 5101 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc 5161 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg 5221 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg 5281 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct 5341 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc 5401 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc 5461 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg 5521 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc 5581 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 5641 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc 5701 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga 5761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 5821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 5881 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 5941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 6001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 6061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 6121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 6181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 6241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 6301 caaaaattta acgcgaattt t //