LOCUS       V029573                 7101 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029573
VERSION     V029573
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3248
                     /label="Daxx(NM_001199733)"
                     /note="Daxx(NM_001199733)"
                     /gene="Daxx"
     CDS             3267..3296
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3315..3338
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3358..3374)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3385..4007
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4156..4744)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5073..5120
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5352..6146)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6181..6538)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6540..6644)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6671..7099
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggccaccgat gacagcatca ttgtacttga tgatgacgat gaagatgaag
     1081 ctgctgctca accagggccc tccaacctac cccccaatcc tgcctcaaca ggacctggtc
     1141 ctggcctgtc tcaacaggcc actggtctct ccgagccccg tgtggatgga gggagcagta
     1201 actccggtag taggaagtgc tacaagttgg ataatgagaa gctctttgaa gagttccttg
     1261 aactgtgtaa gacggagaca tcagaccacc ctgaggtggt tccgttcctc cacaaactgc
     1321 agcagcgtgc ccagtctgtg tttctggcct ctgcagagtt ctgcaacatc ctctccaggg
     1381 ttctggctcg gtctcggaag cggcccgcta agatctatgt gtacattaac gagctctgca
     1441 ctgttcttaa agctcactcc atcaagaaga agttgaactt agctcctgca gcctcaacga
     1501 ccagtgaggc gtcgggccct aaccctccca cagagccccc ctctgacctt acaaacactg
     1561 aaaacactgc ctctgaggcc tcaaggactc gcggttcccg gaggcagatc cagcgcctgg
     1621 agcagctgct ggcactgtac gtagccgaga ttcggcggct gcaggagaag gagttggacc
     1681 tgtcagagct ggatgaccca gactcctcgt atttgcagga ggcccgcttg aagaggaagt
     1741 tgatccgcct cttcgggcgg ttgtgtgagt tgaaggactg ctcttctctg acggggcggg
     1801 tcatagagca gcgaattccg taccgaggca cccggtaccc agaggtcaac aggcgcattg
     1861 aacggctcat taacaagccg gggctggaca ccttccccga ttatggagat gtgctgagag
     1921 ccgtggagaa ggcggcgacc cggcacagcc tgggccttcc cagacagcag cttcagctcc
     1981 tggctcagga tgccttccgg gacgtgggcg tcaggttaca ggagcggcgc cacctggatc
     2041 tcatctacaa ctttggctgt cacctcacag atgactatag gccaggcgtt gaccccgcac
     2101 tgtctgatcc cacattggct cgccgccttc gggaaaatcg aaccttggcc atgaaccggc
     2161 tggatgaggt catctccaag tatgcaatga tgcaagacaa gactgaggag ggcgagagac
     2221 agaagagacg agcccggctc ttaggcaccg ccccccaacc ttcagacccc cccaaagcct
     2281 cctcggaatc tggtgagggt cctagcggaa tggcatccca ggagtgccct actacctcca
     2341 aagctgagac tgatgatgac gatgatgacg atgatgatga cgacgacgaa gataacgagg
     2401 aaagtgagga ggaggaggag gaggaagagg aggagaaaga ggctactgaa gatgaagatg
     2461 aggatctaga acagttgcag gaagatcagg ggggtgatga agaagaggaa ggaggagata
     2521 atgaaggaaa tgagagtccc acatcgcctt cagacttttt ccatagaagg aattcagagc
     2581 ctgcagaagg gctcaggacc cccgaggggc agcaaaagag aggactgaca gagaccccag
     2641 catccccgcc aggggcatcc ctggaccctc ccagcactga cgctgagagc agtggagagc
     2701 agctcctcga gccgctcctg ggagacgaga gtcctgtgtc ccagctcgct gagctagaga
     2761 tggaagcttt gcctgaggaa agggacattt cctcctccag gaaaaagtcg gaagattccc
     2821 tccccaccat cttggaaaat ggggcagctg tggttacctc tacatctgtc aatgggcgtg
     2881 tctcttctca cacttggaga gatgccagtc cccccagcaa gagatttcgg aaggaaaaga
     2941 agcaactggg ctctggactg ttaggaaaca gctatataaa agaaccgatg gcacagcagg
     3001 acagtgggca gaacacaagt gtccagccta tgccatcccc ccccttggcc tctgtggctt
     3061 ctgtcgctga ttcctccaca agggtggact ctcccagcca tgaactggtg accagctctc
     3121 tgtgcagccc ttctccatcc ctgcttctcc agacacccca ggctcagtct ctccggcagt
     3181 gtatttataa gaccagtgtg gccacacagt gcgacccgga ggagatcatc gtgctttcag
     3241 actctgatac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag
     3301 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc
     3361 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     3421 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     3481 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     3541 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     3601 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     3661 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     3721 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     3781 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     3841 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     3901 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     3961 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     4021 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4561 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     4921 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     4981 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     5041 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     5101 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     5161 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     5221 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     5281 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     5341 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     5401 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     5461 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca
     5521 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     5581 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     5641 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     5701 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     5761 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     5821 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     5881 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     5941 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     6001 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     6061 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     6121 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     6181 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6241 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     6301 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     6361 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6421 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     6481 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     6541 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6661 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6721 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6781 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6841 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6901 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6961 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7021 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7081 caaaaattta acgcgaattt t
//
