LOCUS       V031621                 8344 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031621
VERSION     V031621
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3003
                     /label="DPP4(NM_001935)"
                     /note="DPP4(NM_001935)"
                     /gene="DPP4"
     CDS             3010..3036
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3040..3066
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3070..3096
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3120..3708
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3774..3822
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4024..4452
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4466..4795
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4862..5656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5832..5953
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6002..6018)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6026..6042
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6050..6080)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6095..6116
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6404..6992)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7163..8023)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8024..8128)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgaa gacaccgtgg
      721 aaggttcttc tgggactgct gggtgctgct gcgcttgtca ccatcatcac cgtgcccgtg
      781 gttctgctga acaaaggcac agatgatgct acagctgaca gtcgcaaaac ttacactcta
      841 actgattact taaaaaatac ttatagactg aagttatact ccttaagatg gatttcagat
      901 catgaatatc tctacaaaca agaaaataat atcttggtat tcaatgctga atatggaaac
      961 agctcagttt tcttggagaa cagtacattt gatgagtttg gacattctat caatgattat
     1021 tcaatatctc ctgatgggca gtttattctc ttagaataca actacgtgaa gcaatggagg
     1081 cattcctaca cagcttcata tgacatttat gatttaaata aaaggcagct gattacagaa
     1141 gagaggattc caaacaacac acagtgggtc acatggtcac cagtgggtca taaattggca
     1201 tatgtttgga acaatgacat ttatgttaaa attgaaccaa atttaccaag ttacagaatc
     1261 acatggacgg ggaaagaaga tataatatat aatggaataa ctgactgggt ttatgaagag
     1321 gaagtcttca gtgcctactc tgctctgtgg tggtctccaa acggcacttt tttagcatat
     1381 gcccaattta acgacacaga agtcccactt attgaatact ccttctactc tgatgagtca
     1441 ctgcagtacc caaagactgt acgggttcca tatccaaagg caggagctgt gaatccaact
     1501 gtaaagttct ttgttgtaaa tacagactct ctcagctcag tcaccaatgc aacttccata
     1561 caaatcactg ctcctgcttc tatgttgata ggggatcact acttgtgtga tgtgacatgg
     1621 gcaacacaag aaagaatttc tttgcagtgg ctcaggagga ttcagaacta ttcggtcatg
     1681 gatatttgtg actatgatga atccagtgga agatggaact gcttagtggc acggcaacac
     1741 attgaaatga gtactactgg ctgggttgga agatttaggc cttcagaacc tcattttacc
     1801 cttgatggta atagcttcta caagatcatc agcaatgaag aaggttacag acacatttgc
     1861 tatttccaaa tagataaaaa agactgcaca tttattacaa aaggcacctg ggaagtcatc
     1921 gggatagaag ctctaaccag tgattatcta tactacatta gtaatgaata taaaggaatg
     1981 ccaggaggaa ggaatcttta taaaatccaa cttagtgact atacaaaagt gacatgcctc
     2041 agttgtgagc tgaatccgga aaggtgtcag tactattctg tgtcattcag taaagaggcg
     2101 aagtattatc agctgagatg ttccggtcct ggtctgcccc tctatactct acacagcagc
     2161 gtgaatgata aagggctgag agtcctggaa gacaattcag ctttggataa aatgctgcag
     2221 aatgtccaga tgccctccaa aaaactggac ttcattattt tgaatgaaac aaaattttgg
     2281 tatcagatga tcttgcctcc tcattttgat aaatccaaga aatatcctct actattagat
     2341 gtgtatgcag gcccatgtag tcaaaaagca gacactgtct tcagactgaa ctgggccact
     2401 taccttgcaa gcacagaaaa cattatagta gctagctttg atggcagagg aagtggttac
     2461 caaggagata agatcatgca tgcaatcaac agaagactgg gaacatttga agttgaagat
     2521 caaattgaag cagccagaca attttcaaaa atgggatttg tggacaacaa acgaattgca
     2581 atttggggct ggtcatatgg agggtacgta acctcaatgg tcctgggatc gggaagtggc
     2641 gtgttcaagt gtggaatagc cgtggcgcct gtatcccggt gggagtacta tgactcagtg
     2701 tacacagaac gttacatggg tctcccaact ccagaagaca accttgacca ttacagaaat
     2761 tcaacagtca tgagcagagc tgaaaatttt aaacaagttg agtacctcct tattcatgga
     2821 acagcagatg ataacgttca ctttcagcag tcagctcaga tctccaaagc cctggtcgat
     2881 gttggagtgg atttccaggc aatgtggtat actgatgaag accatggaat agctagcagc
     2941 acagcacacc aacatatata tacccacatg agccacttca taaaacaatg tttctcttta
     3001 cctctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat
     3061 tatgctagct atccttatga tgtcccagac tacgcatagt gagggcccga tatctcgaca
     3121 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3181 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3241 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3301 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3361 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3421 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3481 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3541 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3601 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3661 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg
     3721 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     3781 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     3841 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     3901 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     3961 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     4021 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4081 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4141 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     4201 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4261 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4321 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4381 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4441 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4561 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4621 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4681 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4741 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4801 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4861 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4921 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4981 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5041 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5101 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5161 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5221 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5281 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5341 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5401 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5461 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5521 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5581 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5641 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     5701 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5761 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5821 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5941 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6001 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6061 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6121 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6181 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6301 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     6601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6841 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7141 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7201 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7261 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7321 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7381 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7441 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7501 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7561 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7621 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7681 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7741 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7801 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7861 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7921 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7981 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8041 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8101 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8161 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
     8221 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8281 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8341 tagg
//
