LOCUS       V038122                 7140 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038122
VERSION     V038122
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..1866
                     /label="DNMT3L(NM_013369)"
                     /note="DNMT3L(NM_013369)"
                     /gene="DNMT3L"
     CDS             1873..1896
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     misc_feature    1916..2504
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    2570..2618
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      2820..3248
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3262..3591
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3658..4452
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4628..4749
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4798..4814)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4822..4838
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4846..4876)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4891..4912
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5200..5788)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5959..6819)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6820..6924)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggccatccca
      721 gccctggacc cagaggccga gcccagcatg gacgtgattt tggtgggatc cagtgagctc
      781 tcaagctccg tttcacccgg gacaggcaga gatcttattg catatgaagt caaggctaac
      841 cagcgaaata tagaagacat ctgcatctgc tgcggaagtc tccaggttca cacacagcac
      901 cctctgtttg agggagggat ctgcgcccca tgtaaggaca agttcctgga tgccctcttc
      961 ctgtacgacg atgacgggta ccaatcctac tgctccatct gctgctccgg agagacgctg
     1021 ctcatctgcg gaaaccctga ttgcacccga tgctactgct tcgagtgtgt ggatagcctg
     1081 gtcggccccg ggacctcggg gaaggtgcac gccatgagca actgggtgtg ctacctgtgc
     1141 ctgccgtcct cccgaagcgg gctgctgcag cgtcggagga agtggcgcag ccagctcaag
     1201 gccttctacg accgagagtc ggagaatccc cttgagatgt tcgaaaccgt gcctgtgtgg
     1261 aggagacagc cagtccgggt gctgtccctt tttgaagaca tcaagaaaga gctgacgagt
     1321 ttgggctttt tggaaagtgg ttctgacccg ggacaactga agcatgtggt tgatgtcaca
     1381 gacacagtga ggaaggatgt ggaggagtgg ggacccttcg atcttgtgta cggcgccaca
     1441 cctcccctgg gccacacctg tgaccgtcct cccagctggt acctgttcca gttccaccgg
     1501 ctcctgcagt acgcacggcc caagccaggc agccccaggc ccttcttctg gatgttcgtg
     1561 gacaatctgg tgctgaacaa ggaagacctg gacgtcgcat ctcgcttcct ggagatggag
     1621 ccagtcacca tcccagatgt ccacggcgga tccttgcaga atgctgtccg cgtgtggagc
     1681 aacatcccag ccataaggag cagcaggcac tgggctctgg tttcggaaga agaattgtcc
     1741 ctgctggccc agaacaagca gagctcgaag ctcgcggcca agtggcccac caagctggtg
     1801 aagaactgct ttctccccct aagagaatat ttcaagtatt tttcaacaga actcacttcc
     1861 tctttactcg agcaccatca ccatcaccat caccattagg gcccgatatc tcgacaatca
     1921 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1981 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2041 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2101 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2161 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2221 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2281 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     2341 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2401 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2461 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa acgggggagg
     2521 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     2581 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     2641 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     2701 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     2761 cggggcggca ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca
     2821 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2881 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2941 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag
     3001 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3061 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3121 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3181 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3241 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3301 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3361 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3421 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3481 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     3541 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     3601 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     3661 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     3721 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     3781 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     3841 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     3901 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     3961 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4021 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4081 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4141 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     4201 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4261 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4321 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4381 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4441 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     4501 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4561 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4621 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4741 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4801 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4861 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4921 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4981 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     5401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5641 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5881 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     6961 atcgggagat ctcccgatcc cctatggtcg actctcagta caatctgctc tgatgccgca
     7021 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     7081 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
//
