LOCUS V024172 8587 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024172 VERSION V024172 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..3243 /label="DLGAP5(NM_014750)" /note="DLGAP5(NM_014750)" /gene="DLGAP5" CDS 3250..3339 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 3363..3951 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4017..4065 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4267..4695 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4709..5038 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5105..5899 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6075..6196 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6245..6261) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6269..6285 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6293..6323) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6338..6359 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6647..7235) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7406..8266) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8267..8371) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc ttcatcacat 721 tttgccagtc gacacaggaa ggatataagt actgaaatga ttagaactaa aattgctcat 781 aggaaatcac tgtctcagaa agaaaataga cataaggaat acgaacgaaa tagacacttt 841 ggtttgaaag atgtaaacat tccaaccttg gaaggtagaa ttcttgttga attagatgag 901 acatctcaag ggcttgttcc agaaaagacc aatgttaagc caagggcaat gaaaactatt 961 ctaggtgatc aacgaaaaca gatgctccaa aaatacaaag aagaaaagca acttcaaaaa 1021 ttgaaagagc agagagagaa agctaaacga ggaatattta aagtgggtcg ttatagacct 1081 gatatgcctt gttttctttt atcaaaccag aatgctgtga aagctgagcc aaaaaaggct 1141 attccatctt ctgtacggat tacaaggtca aaggccaaag accaaatgga gcagactaag 1201 attgataacg agagtgatgt tcgagcaatc cgacctggtc caagacaaac ttctgaaaag 1261 aaagtgtcag acaaagagaa aaaagttgtg cagcctgtaa tgcccacgtc gttgagaatg 1321 actcgatcag ctactcaagc agcaaagcag gttcccagaa cagtctcatc taccacagca 1381 agaaagccag tcacaagagc tgctaatgaa aacgaaccag aaggaaaggt gccaagtaaa 1441 ggaagacctg ccaaaaatgt agaaacaaaa cccgacaagg gtatttcttg taaagtcgat 1501 agtgaagaaa atactttgaa ttcacaaact aatgcaacaa gtggaatgaa tccagatgga 1561 gtcttatcaa aaatggaaaa cttacctgag ataaatactg caaaaataaa agggaagaat 1621 tcctttgcac ctaaggattt tatgtttcag ccactggatg gtctgaagac ctatcaagta 1681 acacctatga ctcccagaag tgccaatgct tttttgacac ccagttacac ctggactcct 1741 ttaaaaacag aagttgatga gtctcaagca acaaaagaaa ttttggcaca aaaatgtaaa 1801 acttactcta ccaagacaat acagcaagat tcaaataaat tgccatgtcc tttgggtcct 1861 ctaactgttt ggcatgaaga acatgtttta aataaaaatg aagctactac taaaaattta 1921 aatggccttc caataaaaga agtcccatca cttgaaagaa atgaaggtcg aattgctcag 1981 ccccaccatg gtgtgccata tttcagaaat atcctccagt cagaaactga gaaattaact 2041 tcacattgct tcgagtggga caggaaactt gaattggaca ttccagatga tgctaaagat 2101 cttattcgca cagcagttgg tcaaacaaga ctccttatga aggaaaggtt taaacagttt 2161 gaaggactgg ttgatgattg tgaatataaa cgaggtataa aggagactac ctgtacagat 2221 ctggatggat tttgggatat ggttagtttt cagatagaag atgtaatcca caaattcaac 2281 aatctgatca aacttgagga atctgggtgg caagtcaata ataatatgaa tcataatatg 2341 aacaaaaatg tctttaggaa aaaagttgtc tcaggtatag caagtaaacc aaaacaggat 2401 gatgctggaa gaattgcagc gagaaatcgc ctagctgcca taaaaaatgc aatgagagag 2461 agaattaggc aggaagaatg tgctgaaaca gcagtttctg tgataccaaa ggaagttgat 2521 aaaatagtgt tcgatgctgg atttttcaga gttgaaagtc ctgttaaatt attctcagga 2581 ctttctgtct cttctgaagg cccttctcaa agacttggaa cacctaagtc tgtcaacaaa 2641 gctgtatctc agagtagaaa tgagatgggc attccacaac aaactacatc accagaaaat 2701 gccggtcctc agaatacgaa aagtgaacat gtgaagaaga ctttgttttt gagtattcct 2761 gaaagcagga gcagcataga agatgctcag tgtcctggat taccagattt aattgaagaa 2821 aatcatgttg taaataagac agacttgaag gtggattgtt tatccagtga gagaatgagt 2881 ttgcctcttc ttgctggtgg agtagcagat gatattaata ctaacaaaaa agaaggaatt 2941 tcagatgttg tggaaggaat ggaactgaat tcttcaatta catcacagga tgttttgatg 3001 agtagccctg aaaaaaatac agcttcacaa aatagcatct tagaagaagg ggaaactaaa 3061 atttctcagt cagaactatt tgataataaa agtctcacta ctgaatgcca ccttcttgat 3121 tcaccaggtc taaactgcag taatccattt actcagctgg agaggagaca tcaagaacat 3181 gccagacaca tttcttttgg tggtaacctg attacttttt cacctctaca accaggagaa 3241 tttctcgagg aacaaaaact aatatcagag gaagatttgg aacagaaact aatcagtgaa 3301 gaagatttag agcaaaagtt aatttccgag gaggacttat agtgagggcc cgatatctcg 3361 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 3421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 3481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 3541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 3601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 3661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 3721 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 3781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 3841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 3901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaaacg 3961 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc 4021 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc 4081 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc 4141 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag 4201 ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg cagctggggc tctagggggt 4261 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 4321 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 4381 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc 4441 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 4501 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 4561 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 4621 atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa 4681 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 4741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 4801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 4861 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 4921 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 4981 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 5041 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 5101 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 5161 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 5221 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 5281 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 5341 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 5401 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 5461 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5521 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5581 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 5641 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 5701 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 5761 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 5821 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 5881 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc 5941 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 6001 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 6061 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 6121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 6181 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 6241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6301 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 6361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6421 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 6481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 6541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 6601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 6661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6841 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7081 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7201 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7261 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 7561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 7621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 7741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8281 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8341 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8401 tcgacggatc gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga 8461 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 8521 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 8581 gcttagg //