LOCUS V045274 7333 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045274 VERSION V045274 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1989 /label="DDX39B(NM_004640)" /note="DDX39B(NM_004640)" /gene="DDX39B" CDS 1996..2085 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 2109..2697 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2763..2811 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3013..3441 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3455..3784 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3851..4645 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4821..4942 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4991..5007) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5015..5031 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5039..5069) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5084..5105 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5393..5981) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6152..7012) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7013..7117) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc agagaacgat 721 gtggacaatg agctcttgga ctatgaagat gatgaggtgg agacagcagc tgggggagat 781 ggggctgagg cccctgccaa gaaggatgtc aagggctcct atgtctccat ccacagctct 841 ggctttcgtg acttcctgct caagccagag ttgctccggg ccattgtcga ctgtggcttt 901 gagcatccgt cagaagtcca gcatgagtgc atccctcagg ccattctggg aatggatgtc 961 ctgtgccagg ccaagtcggg catgggaaag acagcagtgt ttgtcttggc cacactgcaa 1021 cagctggagc cagttactgg gcaggtgtct gtactggtga tgtgtcacac tcgggagttg 1081 gcttttcaga tcagcaagga atatgagcgc ttctctaaat acatgcccaa tgtcaaggtt 1141 gctgtttttt ttggtggtct gtctatcaag aaggatgaag aggtgctgaa gaagaactgc 1201 ccgcatatcg tcgtggggac tccaggccgt atcctagccc tggctcgaaa taagagcctc 1261 aacctcaaac acattaaaca ctttattttg gatgaatgtg ataagatgct tgaacagctc 1321 gacatgcgtc gggatgtcca ggaaattttt cgcatgaccc cccacgagaa gcaggtcatg 1381 atgttcagtg ctaccttgag caaagagatc cgtccagtct gccgcaagtt catgcaagat 1441 ccaatggaga tcttcgtgga tgatgagacg aagttgacgc tgcatgggtt gcagcagtac 1501 tacgtgaaac tgaaggacaa cgagaagaac cggaagctct ttgaccttct ggatgtcctt 1561 gagttcaacc aggtggtgat ctttgtgaag tctgtgcagc ggtgcattgc cttggcccag 1621 ctactagtgg agcagaactt cccagccatt gccatccacc gtgggatgcc ccaggaggag 1681 aggctttctc ggtatcagca gtttaaagat tttcaacgac gaattcttgt ggctaccaac 1741 ctatttggcc gaggcatgga catcgagcgg gtgaacattg cttttaatta tgacatgcct 1801 gaggattctg acacctacct gcatcgggtg gccagagcag gccggtttgg caccaagggc 1861 ttggctatca catttgtgtc cgatgagaat gatgccaaga tcctcaatga tgtgcaggat 1921 cgctttgagg tcaatattag tgagctgcct gatgagatag acatctcctc ctacattgaa 1981 cagacacggc tcgaggaaca aaaactaata tcagaggaag atttggaaca gaaactaatc 2041 agtgaagaag atttagagca aaagttaatt tccgaggagg acttatagtg agggcccgat 2101 atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 2161 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 2221 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 2281 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 2341 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 2401 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 2461 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 2521 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 2581 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 2641 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 2701 gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 2761 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg 2821 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat 2881 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc 2941 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta 3001 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 3061 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 3121 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag 3181 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 3241 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 3301 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3361 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt 3421 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 3481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3541 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3601 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3661 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3721 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3781 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3841 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3901 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3961 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 4021 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 4081 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 4141 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 4201 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 4261 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 4321 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4381 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4441 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4501 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4561 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4621 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag 4681 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 4741 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 4801 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 4861 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 4921 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 4981 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 5041 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 5101 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 5161 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 5221 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5281 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 5341 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5581 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5821 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 5941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 6061 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 6121 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 6181 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 6241 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 6301 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 6361 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 6421 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 6481 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 6541 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 6601 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 6661 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 6721 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 6781 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 6841 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 6901 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 6961 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 7021 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 7081 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 7141 ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg 7201 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga 7261 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa 7321 gaatctgctt agg //