LOCUS V045254 7330 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045254 VERSION V045254 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1986 /label="DDX39A(NM_005804)" /note="DDX39A(NM_005804)" /gene="DDX39A" CDS 1993..2082 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 2106..2694 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2760..2808 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3010..3438 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3452..3781 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3848..4642 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4818..4939 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4988..5004) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5012..5028 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5036..5066) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5081..5102 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5390..5978) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6149..7009) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7010..7114) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc agaacaggat 721 gtggaaaacg atcttttgga ttacgatgaa gaggaagagc cccaggctcc tcaagagagc 781 acaccagctc cccctaagaa agacatcaag ggatcctacg tttccatcca cagctctggc 841 ttccgggact ttctgctgaa gccggagctc ctgcgggcca tcgtggactg tggctttgag 901 catccttctg aggtccagca tgagtgcatt ccccaggcca tcctgggcat ggacgtcctg 961 tgccaggcca agtccgggat gggcaagaca gcggtcttcg tgctggccac cctacagcag 1021 attgagcctg tcaacggaca ggtgacggtc ctggtcatgt gccacacgag ggagctggcc 1081 ttccagatca gcaaggaata tgagcgcttt tccaagtaca tgcccagcgt caaggtgtct 1141 gtgttcttcg gtggtctctc catcaagaag gatgaagaag tgttgaagaa gaactgtccc 1201 catgtcgtgg tggggacccc gggccgcatc ctggcgctcg tgcggaatag gagcttcagc 1261 ctaaagaatg tgaagcactt tgtgctggac gagtgtgaca agatgctgga gcagctggac 1321 atgcggcggg atgtgcagga gatcttccgc ctgacaccac acgagaagca gtgcatgatg 1381 ttcagcgcca ccctgagcaa ggacatccgg cctgtgtgca ggaagttcat gcaggatccc 1441 atggaggtgt ttgtggacga cgagaccaag ctcacgctgc acggcctgca gcagtactac 1501 gtcaaactca aagacagtga gaagaaccgc aagctctttg atctcttgga tgtgctggag 1561 tttaaccagg tgataatctt cgtcaagtca gtgcagcgct gcatggccct ggcccagctc 1621 ctcgtggagc agaacttccc ggccatcgcc atccaccggg gcatggccca ggaggagcgc 1681 ctgtcacgct atcagcagtt caaggatttc cagcggcgga tcctggtggc caccaatctg 1741 tttggccggg ggatggacat cgagcgagtc aacatcgtct ttaactacga catgcctgag 1801 gactcggaca cctacctgca ccgggtggcc cgggcgggtc gctttggcac caaaggccta 1861 gccatcactt ttgtgtctga cgagaatgat gccaaaatcc tcaatgacgt ccaggaccgg 1921 tttgaagtta atgtggcaga acttccagag gaaatcgaca tctccacata catcgagcag 1981 agccggctcg aggaacaaaa actaatatca gaggaagatt tggaacagaa actaatcagt 2041 gaagaagatt tagagcaaaa gttaatttcc gaggaggact tatagtgagg gcccgatatc 2101 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 2161 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 2221 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 2281 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 2341 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 2401 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 2461 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 2521 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 2581 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 2641 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 2701 acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac 2761 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg 2821 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg 2881 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg 2941 cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg ggctctaggg 3001 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 3061 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 3121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt 3181 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 3241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 3301 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 3361 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac 3421 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 3481 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 3541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3601 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 3661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 3721 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 3781 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc 3841 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 3901 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 3961 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 4021 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 4081 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 4141 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 4201 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 4261 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 4321 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 4381 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 4441 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 4501 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 4561 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4621 ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga 4681 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 4741 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 4801 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4921 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 4981 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 5041 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 5101 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 5161 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 5221 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 5281 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 5341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 5401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 5461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 5521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 5581 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 5641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 5701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 5761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 5821 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 5941 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 6001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 6061 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 6121 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 6181 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 6241 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 6301 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 6361 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 6421 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 6481 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 6541 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 6601 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 6661 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 6721 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 6781 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 6841 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6901 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6961 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 7021 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 7081 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 7141 acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta caatctgctc 7201 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 7261 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa 7321 tctgcttagg //