LOCUS       V042263                 8686 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042263
VERSION     V042263
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3345
                     /label="DCAF6(NM_018442)"
                     /note="DCAF6(NM_018442)"
                     /gene="DCAF6"
     CDS             3352..3378
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3382..3408
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3412..3438
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3462..4050
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4116..4164
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4366..4794
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4808..5137
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5204..5998
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6174..6295
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6344..6360)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6368..6384
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6392..6422)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6437..6458
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6746..7334)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7505..8365)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8366..8470)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc tcggggtggc
      721 tcctacccac acctgttgtg ggacgtgagg aaaaggtccc tcgggctgga ggacccgtcc
      781 cggctgcgga gtcgctacct gggaagaaga gaatttatcc aaagattaaa acttgaagca
      841 acccttaatg tgcatgatgg ttgtgttaat acaatctgtt ggaatgacac tggagaatat
      901 attttatctg gctcagatga caccaaatta gtaattagta atccttacag cagaaaggtt
      961 ttgacaacaa ttcgttcagg gcaccgagca aacatattta gtgcaaagtt cttaccttgt
     1021 acaaatgata aacagattgt atcctgctct ggagatggag taatatttta taccaacgtt
     1081 gagcaagatg cagaaaccaa cagacaatgc caatttacgt gtcattatgg aactacttat
     1141 gagattatga ctgtacccaa tgacccttac acttttctct cttgtggtga agatggaact
     1201 gttaggtggt ttgatacacg catcaaaact agctgcacaa aagaagattg taaagatgat
     1261 attttaatta actgtcgacg tgctgccacg tctgttgcta tttgcccacc aataccatat
     1321 taccttgctg ttggttgttc tgacagctca gtacgaatat atgatcggcg aatgctgggc
     1381 acaagagcta cagggaatta tgcaggtcga gggactactg gaatggttgc ccgttttatt
     1441 ccttcccatc ttaataataa gtcctgcaga gtgacatctc tgtgttacag tgaagatggt
     1501 caagagattc tcgttagtta ctcttcagat tacatatatc tttttgaccc gaaagatgat
     1561 acagcacgag aacttaaaac tccttctgcg gaagagagaa gagaagagtt gcgacaacca
     1621 ccagttaagc gtttgagact tcgtggtgat tggtcagata ctggacccag agcaaggccg
     1681 gagagtgaac gagaacgaga tggagagcag agtcccaatg tgtcattgat gcagagaatg
     1741 tctgatatgt tatcaagatg gtttgaagaa gcaagtgagg ttgcacaaag caatagagga
     1801 cgaggaagat ctcgacccag aggtggaaca agtcaatcag atatttcaac tcttcctacg
     1861 gtcccatcaa gtcctgattt ggaagtgagt gaaactgcaa tggaagtaga tactccagct
     1921 gaacaatttc ttcagccttc tacatcctct acaatgtcag ctcaggctca ttcgacatca
     1981 tctcccacag aaagccctca ttctactcct ttgctatctt ctccagacag tgaacaaagg
     2041 cagtctgttg aggcatctgg acaccacaca catcatcagt ctgattctcc ttcttctgtg
     2101 gttaacaaac agctcggatc catgtcactt gacgagcaac aggataacaa taatgaaaag
     2161 ctgagcccca aaccagggac aggtgaacca gttttaagtt tgcactacag cacagaagga
     2221 acaactacaa gcacaataaa actgaacttt acagatgaat ggagcagtat agcatcaagt
     2281 tctagaggaa ttgggagcca ttgcaaatct gagggtcagg aggaatcttt cgtcccacag
     2341 agctcagtgc aaccaccaga aggagacagt gaaacaaaag ctcctgaaga atcatcagag
     2401 gatgtgacaa aatatcagga aggagtatct gcagaaaacc cagttgagaa ccatatcaat
     2461 ataacacaat cagataagtt cacagccaag ccattggatt ccaactcagg agaaagaaat
     2521 gacctcaatc ttgatcgctc ttgtggggtt ccagaagaat ctgcttcatc tgaaaaagcc
     2581 aaggaaccag aaacttcaga tcagactagc actgagagtg ctaccaatga aaataacacc
     2641 aatcctgagc ctcagttcca aacagaagcc actgggcctt cagctcatga agaaacatcc
     2701 accagggact ctgctcttca ggacacagat gacagtgatg atgacccagt cctgatccca
     2761 ggtgcaaggt atcgagcagg acctggtgat agacgctctg ctgttgcccg tattcaggag
     2821 ttcttcagac ggagaaaaga aaggaaagaa atggaagaat tggatacttt gaacattaga
     2881 aggccgctag taaaaatggt ttataaaggc catcgcaact ccaggacaat gataaaagaa
     2941 gccaatttct ggggtgctaa ctttgtaatg agtggttctg actgtggcca cattttcatc
     3001 tgggatcggc acactgctga gcatttgatg cttctggaag ctgataatca tgtggtaaac
     3061 tgcctgcagc cacatccgtt tgacccaatt ttagcctcat ctggcataga ttatgacata
     3121 aagatctggt caccattaga agagtcaagg atttttaacc gaaaacttgc tgatgaagtt
     3181 ataactcgaa acgaactcat gctggaagaa actagaaaca ccattacagt tccagcctct
     3241 ttcatgttga ggatgttggc ttcacttaat catatccgag ctgaccggtt ggagggtgac
     3301 agatcagaag gctctggtca agagaatgaa aatgaggatg aggaactcga gtacccatac
     3361 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat
     3421 gatgtcccag actacgcata gtgagggccc gatatctcga caatcaacct ctggattaca
     3481 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3541 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3601 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3661 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3721 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3781 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3841 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     3901 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3961 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4021 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg
     4081 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     4141 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     4201 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     4261 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     4321 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4381 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4441 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4501 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
     4561 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4621 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4681 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
     4741 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4801 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4861 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4921 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4981 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5041 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5101 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5161 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5221 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5281 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5341 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5401 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5461 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5521 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5581 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5641 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5701 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     5761 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5821 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5881 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5941 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6001 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6061 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6121 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6181 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6241 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6301 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6361 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6421 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6481 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6541 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6601 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6661 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6721 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6781 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6841 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6901 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     6961 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7021 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7081 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7141 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7201 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7261 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7321 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7381 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7441 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7501 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7561 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7621 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7681 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7741 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7801 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7861 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7921 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7981 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8041 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8101 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8161 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8221 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8281 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8341 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8401 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8461 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8521 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8581 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8641 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg
//
