LOCUS       V030857                 6309 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030857
VERSION     V030857
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2456
                     /label="Cpe(NM_013494)"
                     /note="Cpe(NM_013494)"
                     /gene="Cpe"
     CDS             2475..2504
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2523..2546
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2566..2582)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2593..3215
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3364..3952)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4281..4328
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4560..5354)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5389..5746)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5748..5852)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5879..6307
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggccgggcgc ggaggacggg tgctgctggc gctgtgtgcc gcgctggtgg
     1081 ccggcgggtg gctgctgacg gctgaagccc aggagcccgg ggcgccagcg gctggcatga
     1141 ggcgccgccg gcggctccag caagaggacg gcatctcctt cgagtaccac cgctatccag
     1201 agctgcgcga ggcgctggtg tccgtatggc tgcagtgcac cgccatcagc agaatctaca
     1261 cagtggggcg cagcttcgag ggccgggagc tcctggtcat cgagctgtct gacaaccccg
     1321 gggtccatga gccgggtgaa cctgaattta aatacattgg gaacatgcat ggtaatgagg
     1381 cggttggacg ggaactgctt attttcttgg cccagtacct gtgtaacgag taccagaaag
     1441 gcaatgagac aattgtcaac ctgatccaca gcacccgaat tcatatcatg ccctccttga
     1501 accccgacgg ctttgagaaa gccgcatcgc agcccggcga gctgaaggac tggtttgtgg
     1561 gccgcagcaa cgcccaggga atagatctga accgtaactt cccagacctg gacaggatcg
     1621 tgtatgttaa tgagaaagaa ggcggtccta acaatcacct gctgaagaat ctgaagaaaa
     1681 ttgtggacca aaattcaaag cttgcccccg agaccaaggc tgtcattcac tggatcatgg
     1741 acattccatt tgtgctttct gccaatctgc acggaggaga ccttgtggct aattacccat
     1801 atgatgagac acggagcggt actgctcacg aatacagttc ctgccctgat gacgcaattt
     1861 tccaaagctt ggctcgcgcg tactcttctt tcaacccagt catgtctgac cccaatcgac
     1921 ctccctgtcg caagaatgac gatgacagca gctttgtaga tggaacgacc aatggtggtg
     1981 catggtacag cgtccccggt ggaatgcaag acttcaatta cctgagcagc aactgcttcg
     2041 agatcactgt ggagcttagc tgtgagaagt tcccaccgga agagactctc aaaagctact
     2101 gggaagataa caaaaactcc ctcatcagct acctggagca gatacaccga ggtgttaaag
     2161 ggtttgtccg tgaccttcag ggtaacccga ttgccaacgc aaccatctct gtggacggga
     2221 tagaccatga tgtcacctcg gctaaggatg gggattactg gcgattgctt gctcctggaa
     2281 actataaact tacagcctcc gctcctggct acctggcaat cacaaagaaa gtggcagttc
     2341 cttttagccc tgctgttggg gtggactttg agcttgagtc tttctctgaa aggaaggagg
     2401 aggagaagga agaattgatg gagtggtgga aaatgatgtc agaaactttg aattttacgc
     2461 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc
     2521 tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc
     2581 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     2641 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     2701 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     2761 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     2821 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     2881 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     2941 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     3001 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     3061 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     3121 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     3181 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt
     3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4081 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac
     4141 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
     4201 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4261 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt
     4321 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4381 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     4441 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     4501 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     4561 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     4621 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     4681 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat
     4741 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     4801 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg
     4861 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     4921 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4981 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     5041 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     5101 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     5161 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     5221 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     5281 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     5341 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc
     5401 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     5461 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     5521 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     5581 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     5641 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     5701 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt
     5761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5881 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5941 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6001 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6061 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6121 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6181 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6241 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6301 gcgaatttt
//
