LOCUS       V031279                 7959 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031279
VERSION     V031279
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4088
                     /label="Cntn1(NM_001358051)"
                     /note="Cntn1(NM_001358051)"
                     /gene="Cntn1"
     CDS             4125..4154
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4173..4196
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4216..4232)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4243..4865
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5014..5602)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5931..5978
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6210..7004)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7039..7396)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7398..7502)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7529..7957
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaaaatgcca ttgctggtca gccatctcct gctaatctcc ttaacatctt
     1081 gtctaggaga ctttacctgg cacagaagat atggtcatgg agtgtctgag gaggacaaag
     1141 gatttgggcc catttttgaa gagcagccaa tcaataccat ttacccagaa gagtcactgg
     1201 aaggaaaagt gtcactcaac tgccgggcac gcgccagccc atttccagtt tacaaatgga
     1261 gaatgaataa cggggacgtg gacctgacaa acgatcggta cagtatggtc ggaggaaacc
     1321 ttgttatcaa caacccggac aaacagaaag acgctggggt gtactactgc ctggcatcca
     1381 acaactacgg aatggtcagg agcacggagg caactctgag cttcgggtat ctcgacccct
     1441 ttcctcccga ggagcgtccg gaagtgaagg tgaaggaagg gaagggcatg gtgcttctct
     1501 gtgatcctcc ctaccacttc ccagatgatc ttagctaccg ctggcttctg aatgaatttc
     1561 ctgtatttat caccatggat aagcgaagat ttgtgtctca gaccaacggc aatctctata
     1621 tcgcaaatgt cgagtcttct gacagaggca actactcctg ctttgtgtcc agtccttcta
     1681 ttacaaagag tgtgttcagc aagtttattc ccctcattcc gattcctgaa cgaacaacaa
     1741 agccatatcc tgctgatatt gtggtgcagt tcaaggacat atacaccatg atgggccaaa
     1801 atgtgacttt agagtgtttc gctctaggga accctgttcc tgatatccgg tggcggaagg
     1861 tgctagaacc aatgcccagc actgccgaga taagcacgtc aggggctgtc ctcaagatct
     1921 tcaacatcca gctagaagat gaaggcctct acgagtgtga ggctgagaac atccggggaa
     1981 aggacaagca ccaggcaaga atctatgttc aagcgtttcc tgagtgggta gagcatatca
     2041 atgacactga ggtggacata ggcagtgacc tctactggcc ttgcatagcc acagggaagc
     2101 ccattcctac catcaggtgg ctgaaaaatg gatactcgta tcacaaaggg gaactgagac
     2161 tgtatgatgt gacttttgaa aatgccggga tgtaccagtg catagcagaa aacgcgtatg
     2221 ggtccattta tgccaacgct gagctcaaga tcctggcttt agctccaact tttgaaatga
     2281 atcctatgaa gaaaaagatc ttggctgcga aaggtggaag ggttgtcatt gaatgtaaac
     2341 ccaaggccgc accaaaaccc aagttttcat ggagcaaagg gacagagtgg ctcgtcaaca
     2401 gcagcagaat actcatatgg gaagatggta gcttggaaat caataatatt acaagaaatg
     2461 atggtggaat ctatacatgc tttgcagaaa ataacagagg aaaagctaat agcactggga
     2521 ctctggtgat cacaaatccc acacgaatta ttttggcccc aatcaatgct gacatcactg
     2581 ttggggaaaa tgccaccatg cagtgtgctg cgtctttcga tcctgccttg gacctcacgt
     2641 ttgtgtggtc cttcaatggc tacgtgattg actttaacaa ggaaattacg catatccatt
     2701 accagaggaa ttttatgctc gacgccaatg gggaactgct catccgaaat gcgcagctga
     2761 aacacgcagg ccggtacacg tgcacggcgc agacaatcgt ggacaattcc tcggcttccg
     2821 ctgaccttgt cgttcgaggc cccccaggtc ccccaggagg cctgagaata gaagacatca
     2881 gagctacctc tgtggcactg acttggagcc gtggttccga caatcacagc cctatctcta
     2941 aatacacgat ccagaccaag acgattctct cagatgactg gaaagatgcg aagacagatc
     3001 ccccaatcat tgaagggaac atggagtcag ctaaagcagt ggatttgatc ccttggatgg
     3061 agtatgaatt tcgagtggtg gcgaccaata ctttagggac gggagagccc agcataccct
     3121 caaacaggat caaaacagac ggtgctgcac caaatgtggc tccctcagat gtaggcggtg
     3181 ggggcggaac caacagagag ctgacaataa cgtgggcgcc tttgtcgaga gagtaccatt
     3241 acggtaacaa ttttggttac atagtggcgt ttaagccatt tgatggagaa gagtggaaga
     3301 aagtgactgt gactaaccca gacactggcc gctacgtcca caaagatgag accatgaccc
     3361 cctccaccgc gtttcaagtc aaagtgaaag ccttcaacaa taaaggagat gggccctaca
     3421 gcctcgtagc tgtcatcaac tctgcacaag atgctcccag tgaagccccc acagaggtag
     3481 gggtcaaagt gttatcatcc tctgagatat ctgtccattg gaagcatgtg ttagagaaaa
     3541 ttgtggaaag ctatcagatt cggtactggg ctgggcatga caaagaagca gccgcacaca
     3601 gagttcaggt caccagccag gagtactcag ccagactgga gaaccttctt cccgacaccc
     3661 agtacttcat tgaagtggga gcctgcaaca gtgctggatg cggaccttcc agtgatgtga
     3721 tcgagacctt caccagaaaa gcccctccta gccaaccacc aaggatcatc agttcggtga
     3781 gatctggctc acgatatata atcacatggg atcacgttgt ggcactatcc aatgaatcta
     3841 ctgtgacagg ctacaagata ctctatagac cggatggcca acatgacggc aagctgttct
     3901 caacccataa acactccata gaagtcccca tccccagaga cggagagtat gttgtcgagg
     3961 ttcgagcaca cagcgacggg ggagacggag ttgtgtctca agtcaaaatt tcaggcgtgt
     4021 ccacgctgtc ttcgagcctc ctcagcttgc tcctgccctc ccttggcttt cttgtctact
     4081 cggaattcag cggaccgagc ggaccgacgc gtacgcggcc gctcgagcag aaactcatct
     4141 cagaagagga tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt
     4201 aaacggccgg ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac
     4261 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     4321 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     4381 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     4441 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     4501 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     4561 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     4621 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     4681 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     4741 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     4801 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     4861 gaattgggta cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4921 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5521 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5581 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5641 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5701 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     5761 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     5821 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     5881 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     5941 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     6001 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     6061 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     6121 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     6181 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     6241 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     6301 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc
     6361 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     6421 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     6481 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     6541 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     6601 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     6661 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     6721 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     6781 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     6841 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     6901 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     6961 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     7021 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     7081 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     7141 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     7201 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     7261 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     7321 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     7381 gttctttccg cctcaggact cttccttttt caatattatt gaagcattta tcagggttat
     7441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7501 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     7561 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7621 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7681 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7741 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7801 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7861 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7921 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
