LOCUS       V031165                10014 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031165
VERSION     V031165
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..6161
                     /label="Chd1(NM_007690)"
                     /note="Chd1(NM_007690)"
                     /gene="Chd1"
     CDS             6180..6209
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             6228..6251
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(6271..6287)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    6298..6920
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(7069..7657)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    7986..8033
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(8265..9059)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(9094..9451)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(9453..9557)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      9584..10012
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaatggacac agtgatgaag aaagtgttag aaatggcagc ggagaatcaa
     1081 gtcagtcagg tgatgattgt gggtcagcat caggctctgg atctggctcg agttctggca
     1141 gcagcagtga cggaagcagc agccaatccg ggagcagcga ctctgattct ggctctgact
     1201 caggaagtca atcagagtct gaatcagaca catcccgaga gaacaaggtt caagcaaaac
     1261 caccaaaagt cgacggagcc gagttttgga aatctagccc cagtattctg gctgtccaga
     1321 gatctgcaat gcttaggaag cagccacagc aggcccagca gcagcgccca gcttcatcta
     1381 atagtggatc cgaagaagac tcgtccagca gtgaagactc cgacgactcg tccagcggtg
     1441 ccaagaggaa gaagcacaat gatgaagact ggcagatgtc tgggtccgga tctccatctc
     1501 agtctggttc agactcagaa tctgaagaag agcgagataa aagcagctgc gacgggacag
     1561 agtccgacta cgagccgaaa aacaaagtca gaagccgaaa gcctcagaat agatctaagt
     1621 caaaaaatgg gaaaaaaatt cttggacaaa aaaagagaca gattgattca tctgaggatg
     1681 aagatgatga agattatgat aatgataaac gaagctctcg ccgccaagcc accgtcaatg
     1741 tgagctacaa ggaggatgaa gaaatgaaaa ccgactccga tgacctgctg gaggtctgcg
     1801 gcgaggacgt ccctcagcct gaggacgagg agtttgagac aatagagagg gttatggatt
     1861 gcagagtggg gcggaaagga gctactggtg ctactacaac catttatgct gtcgaagcag
     1921 atggtgaccc aaatgcagga tttgaaagaa acaaagagcc aggagacata cagtatttaa
     1981 ttaagtggaa aggatggtct cacatccaca acacatggga gacagaagag accctgaagc
     2041 agcagaacgt tagagggatg aaaaaattgg ataattataa gaaaaaagat caagagacga
     2101 aacgatggct gaaaaatgct tctccagaag atgtggaata ttataattgc cagcaagagc
     2161 ttacagatga tctacacaaa cagtatcaga tagtggagcg cataattgct cattccaatc
     2221 aaaaatcagc agctggtctt cctgattatt attgcaaatg gcaggggctt ccatactcag
     2281 agtgcagctg ggaggatgga gctctcattt ccaaaaagtt tcagacatgc atcgatgaat
     2341 attttagcag gaatcagtca aaaacgacac cttttaaaga ttgcaaagta ttgaaacaaa
     2401 gaccaagatt tgtagctctg aagaaacaac catcctatat tggaggacat gagggcttag
     2461 aactgagaga ctatcagctg aatggtttaa actggctcgc tcactcttgg tgcaaaggaa
     2521 atagttgcat acttgctgat gaaatgggcc ttgggaaaac aatacagacg atctcatttt
     2581 tgaactattt gttccatgaa catcagttat atgggccgtt tctactagtt gtcccgctct
     2641 ccactctgac ttcctggcag agggagatac agacgtgggc gtctcagatg aatgctgtgg
     2701 tttacttagg cgacattaac agcagaaaca tgataagaac tcatgaatgg atgcatcccc
     2761 agaccaaacg gttaaaattt aatatacttt taacaacgta tgaaatttta ttgaaggata
     2821 aggcattcct tggtggtctg aattgggcat tcataggtgt tgatgaagcg catcgattaa
     2881 agaatgatga ttcccttctg tacaaaactt taatcgactt taaatctaac catcgccttc
     2941 tgatcactgg aacccctcta cagaactccc tgaaggagct ctggtcactg ctgcacttca
     3001 ttatgccgga gaagttttct tcatgggaag attttgaaga agaacatggc aaaggcagag
     3061 aatatggtta tgcaagcctc cacaaggagc ttgagccatt tctgttacgc agagttaaga
     3121 aagatgtgga aaaatctctt cctgccaagg tggagcagat tttaagaatg gagatgagtg
     3181 ctttacagaa gcaatattac aagtggattt taactaggaa ttataaagcc ctcagcaaag
     3241 gttccaaggg cagtacctca ggctttttga acattatgat ggagctaaag aaatgttgta
     3301 accattgcta cctcattaaa ccaccagata ataatgaatt ctataataaa caggaggcct
     3361 tacaacactt aatccgtagt agcggaaaac tcatccttct cgacaagctg ttgattcgcc
     3421 taagagaaag aggcaaccga gtgctcattt tctctcagat ggtgcggatg ttagacatac
     3481 tcgcagagta tttgaagtac cgccagttcc cctttcaaag attagatgga tcgataaaag
     3541 gggagctgag gaagcaggct ttggatcact ttaatgccga gggctcagag gatttctgct
     3601 ttttgctttc tacccgagct gggggtttag gcattaacct agcctctgct gacaccgttg
     3661 ttatattcga ttctgattgg aatccacaaa atgatcttca ggctcaggca agagctcacc
     3721 gaattggaca gaagaaacag gtgaatatat atcgcttggt tacaaaggga tcagttgaag
     3781 aagatattct tgaaagggcc aaaaagaaaa tggttttgga tcatcttgtt attcaaagaa
     3841 tggataccac tgggaagaca gtgctgcaca caggctcggc tccgtcaagt tccaccccct
     3901 tcaataaaga ggagttatcc gccattttaa aattcggtgc tgaggagctc tttaaggagc
     3961 ctgaaggaga ggagcaggag ccacaggaaa tggatatcga tgaaatcctg aagagagccg
     4021 agactcacga gaacgagcca ggcccactga gtgtggggga tgagctgctt tctcagttta
     4081 aggttgccaa tttttcaaat atggatgaag atgacattga attggaacct gaaagaaatt
     4141 caaagaactg ggaggagatc attccagaag agcaaagacg gcgactagaa gaggaggaga
     4201 gacaaaagga actggaggaa atttatatgc tcccgagaat gagaaactgt gcaaagcaga
     4261 taagtttcaa tggaagtgaa gggaggcgga gtagaagcag gagatattct ggatctgata
     4321 gtgattcaat ctcggaaagg aaacggccga agaaacgtgg gcgaccccgc actatccctc
     4381 gggagaatat taaaggattt agtgatgcgg agattcggcg gtttatcaag agctataaga
     4441 aatttggtgg ccccctggag aggttagatg caattgctcg agatgctgaa ttggttgata
     4501 agtcagaaac agatctcaga agactaggag aactggtgca taatggttgt gttaaagctt
     4561 taaaagacag ttcttcagga acagagcgag caggtggcag acttggaaaa gtgaaggggc
     4621 caacattccg catctctgga gtccaagtga atgccaagct ggtcattgcc catgaggatg
     4681 agctgatccc tctgcataag tccatccctt cggacccgga ggagaggaag cagtatacaa
     4741 tcccctgcca tacaaaggct gcgcactttg atatagactg gggcaaagaa gatgattcta
     4801 atttgttaat tggtatctat gagtatggct atggaagctg ggaaatgatt aaaatggatc
     4861 cagacctcag tttaacacac aagattcttc cagatgatcc tgataaaaaa ccacaagcaa
     4921 aacagttaca gacccgtgca gactacctca tcaaactact tagcagagat cttgcaaaaa
     4981 gagaggctca gagactttgt ggtgcgggag gttcaaagag gagaaaaacg agagctaaaa
     5041 agagtaaggc aatgaagtcc atcaaagtga aagaggaaat aaagagtgac tcgtcgcccc
     5101 tgccttcaga gaagtctgac gaggatgacg ataaactgaa tgactccaag cctgaaagta
     5161 aagaccgatc caaaaagtct gtagtgtccg atgctcccgt tcacatcact gcgagtggag
     5221 agcccgttcc catagctgaa gagtctgaag agctggatca gaagacattc agtatttgta
     5281 aagaaagaat gagaccggtg aaagcagctt tgaaacaact tgacaggcct gagaaaggcc
     5341 tttcagaaag agagcagctg gaacacacta gacagtgctt aatcaagatc ggagaccata
     5401 tcactgaatg cttgaaggaa tattccaatc ctgaacaaat taagcagtgg aggaaaaacc
     5461 tgtggatttt tgtatctaag tttactgagt ttgatgcaag gaaattacat aaattatata
     5521 agcatgctat taaaaaacga caagaatctc agcaaaacag tgaccagaat agcaatgttg
     5581 ctaccactca tgtgattagg aatccagata tggaaaggtt aaaagagaat acaaatcatg
     5641 atgacagtag cagggacagc tattcttctg acagacactt atctcagtac catgatcatc
     5701 acaaggaccg ccatcaggga gattcttata aaaagagtga ctctcggaag agaccctact
     5761 cctcatttag caatggcaaa gaccaccgcg agtgggatca ctacaggcaa gacagcaggt
     5821 actatagtga ccgagagaaa cacagaaaac tggatgacca caggagtcga gagcacaggc
     5881 caagtttgga aggaggctta aaggacaggt gtcactctga ccaccgatct cactcggacc
     5941 atcgaatgca ctcagaccat cgctcaagct ccgagcacac acatcataaa tcctccaggg
     6001 attatcggta tctctcagat tggcagttgg accaccgagc tgccagcagt ggccctaggt
     6061 cacctttaga tcagaggtct ccatatggct ccaggtcccc atttgaacat tcagctgaac
     6121 acagaagtac gcctgaacac acctggagta gtcggaagac aacgcgtacg cggccgctcg
     6181 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg
     6241 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg
     6301 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     6361 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     6421 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     6481 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     6541 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     6601 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     6661 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     6721 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     6781 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc
     6841 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact
     6901 cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa
     6961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7501 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7801 tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     7861 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     7921 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     7981 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc
     8041 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc
     8101 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     8161 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     8221 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     8281 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     8341 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     8401 tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     8461 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     8521 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     8581 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     8641 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     8701 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     8761 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     8821 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc
     8881 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     8941 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     9001 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     9061 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
     9121 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     9181 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     9241 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     9301 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     9361 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     9421 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
     9481 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9541 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     9601 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9661 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9721 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9781 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9841 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9901 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9961 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt
//
