LOCUS       V044093                 8131 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044093
VERSION     V044093
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2226
                     /label="Cgas(NM_173386)"
                     /note="Cgas(NM_173386)"
                     /gene="Cgas"
     CDS             2233..2889
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK*"
                     /label="GST"
     misc_feature    2907..3495
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3561..3609
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3811..4239
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4253..4582
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4649..5443
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5619..5740
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5789..5805)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5813..5829
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5837..5867)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5882..5903
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6191..6779)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6950..7810)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7811..7915)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga agatccgcgt
      721 agaaggacga cggcgccacg cgctaagaag ccgtccgcga agcgcgcccc gacgcagccc
      781 agcaggacca gggcccacgc ggaaagctgc ggcccgcaaa ggggggctcg atcgcggcgg
      841 gcggagcgtg acggggacac cacggagaag ccacgtgccc cagggccccg agtgcatcca
      901 gcaagggcca ctgagctcac caaagatgca cagccctcgg ccatggacgc ggcaggagcc
      961 accgcgcggc ctgccgtccg ggtgccccag cagcaggcca tcctggatcc ggagctgccc
     1021 gccgtacggg agccccagcc gcccgcggat cccgaggcgc ggaaagtcgt aaggggacct
     1081 agccacagaa ggggcgcgcg ctccaccggg cagcccagag cgccgcgagg gtccaggaag
     1141 gaaccggaca agctaaagaa ggtgctggac aaattgagat tgaaacgcaa agatatctcg
     1201 gaggcggccg agacggtgaa taaagttgtg gaacgcctgc tgcgcagaat gcagaaacgg
     1261 gagtcggagt tcaaaggtgt ggagcagctg aacactggca gctactatga acatgtgaag
     1321 atttctgctc ctaatgaatt tgatgttatg tttaaactgg aagtccccag gattgagcta
     1381 caagaatatt atgaaacagg tgctttctat cttgtgaaat tcaaaagaat tccacgagga
     1441 aatccgctga gtcatttctt agaaggggaa gtattatcag ctaccaagat gctgtcaaag
     1501 tttaggaaaa tcattaaaga agaagttaaa gaaatcaaag atatagatgt cagtgtggag
     1561 aaggaaaaac caggaagccc tgctgtaaca cttcttatca ggaaccctga agaaatctct
     1621 gtggatataa ttctggcttt ggagtcaaaa ggcagctggc ctattagtac caaagaagga
     1681 ctacctattc aaggctggct gggcacaaaa gtgaggacca atctaagacg agagccgttt
     1741 tatctcgtac ccaagaatgc aaaggatgga aatagttttc aaggagagac ctggcgcctc
     1801 tctttctctc acactgaaaa gtacattttg aataatcacg ggatagagaa aacatgctgt
     1861 gaatcttccg gagcaaaatg ctgcagaaaa gaatgtttaa aattaatgaa ataccttttg
     1921 gaacagttga aaaaagagtt tcaagagctg gatgcattct gttcctacca tgtgaaaact
     1981 gccatctttc acatgtggac ccaggacccg caggacagtc agtgggaccc caggaacctc
     2041 agctcctgct tcgataagtt gttagcattc tttcttgagt gcctcaggac agagaaactg
     2101 gatcattatt ttattccaaa gttcaatcta ttctctcaag aactaattga ccgaaaaagt
     2161 aaagaatttc tatcgaagaa aattgaatat gaaagaaata atgggtttcc aatttttgac
     2221 aagcttctcg agatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc
     2281 actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat
     2341 gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct
     2401 tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct
     2461 gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa
     2521 ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa
     2581 actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt
     2641 ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat
     2701 gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta
     2761 gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc
     2821 aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct
     2881 ccaaaatgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     2941 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3001 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3061 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3121 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     3181 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3241 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3301 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3361 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3421 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3481 gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc
     3541 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     3601 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     3661 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     3721 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc
     3781 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3841 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3901 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3961 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4021 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4081 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4141 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt
     4201 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4261 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4321 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4381 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4501 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4561 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4621 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     4681 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4741 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4801 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     4861 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4921 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4981 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5041 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5101 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5161 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5221 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5281 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5341 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5401 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     5461 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5521 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5581 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5641 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5701 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     5761 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5821 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5881 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5941 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6001 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6061 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6121 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6181 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6241 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6301 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6361 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     6421 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6481 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6541 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6601 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6661 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6721 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6781 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6841 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6961 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7021 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7081 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7141 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7201 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7261 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7321 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7381 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7441 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7501 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7561 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7621 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7681 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7741 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7801 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7861 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7921 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc
     7981 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8041 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8101 gaccgacaat tgcatgaaga atctgcttag g
//
