LOCUS V044093 8131 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044093 VERSION V044093 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2226 /label="Cgas(NM_173386)" /note="Cgas(NM_173386)" /gene="Cgas" CDS 2233..2889 /codon_start=1 /gene="" /product="glutathione S-transferase from Schistosoma japonicum" /transl_table=1 /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK*" /label="GST" misc_feature 2907..3495 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3561..3609 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3811..4239 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4253..4582 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4649..5443 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5619..5740 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5789..5805) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5813..5829 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5837..5867) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5882..5903 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6191..6779) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6950..7810) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7811..7915) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga agatccgcgt 721 agaaggacga cggcgccacg cgctaagaag ccgtccgcga agcgcgcccc gacgcagccc 781 agcaggacca gggcccacgc ggaaagctgc ggcccgcaaa ggggggctcg atcgcggcgg 841 gcggagcgtg acggggacac cacggagaag ccacgtgccc cagggccccg agtgcatcca 901 gcaagggcca ctgagctcac caaagatgca cagccctcgg ccatggacgc ggcaggagcc 961 accgcgcggc ctgccgtccg ggtgccccag cagcaggcca tcctggatcc ggagctgccc 1021 gccgtacggg agccccagcc gcccgcggat cccgaggcgc ggaaagtcgt aaggggacct 1081 agccacagaa ggggcgcgcg ctccaccggg cagcccagag cgccgcgagg gtccaggaag 1141 gaaccggaca agctaaagaa ggtgctggac aaattgagat tgaaacgcaa agatatctcg 1201 gaggcggccg agacggtgaa taaagttgtg gaacgcctgc tgcgcagaat gcagaaacgg 1261 gagtcggagt tcaaaggtgt ggagcagctg aacactggca gctactatga acatgtgaag 1321 atttctgctc ctaatgaatt tgatgttatg tttaaactgg aagtccccag gattgagcta 1381 caagaatatt atgaaacagg tgctttctat cttgtgaaat tcaaaagaat tccacgagga 1441 aatccgctga gtcatttctt agaaggggaa gtattatcag ctaccaagat gctgtcaaag 1501 tttaggaaaa tcattaaaga agaagttaaa gaaatcaaag atatagatgt cagtgtggag 1561 aaggaaaaac caggaagccc tgctgtaaca cttcttatca ggaaccctga agaaatctct 1621 gtggatataa ttctggcttt ggagtcaaaa ggcagctggc ctattagtac caaagaagga 1681 ctacctattc aaggctggct gggcacaaaa gtgaggacca atctaagacg agagccgttt 1741 tatctcgtac ccaagaatgc aaaggatgga aatagttttc aaggagagac ctggcgcctc 1801 tctttctctc acactgaaaa gtacattttg aataatcacg ggatagagaa aacatgctgt 1861 gaatcttccg gagcaaaatg ctgcagaaaa gaatgtttaa aattaatgaa ataccttttg 1921 gaacagttga aaaaagagtt tcaagagctg gatgcattct gttcctacca tgtgaaaact 1981 gccatctttc acatgtggac ccaggacccg caggacagtc agtgggaccc caggaacctc 2041 agctcctgct tcgataagtt gttagcattc tttcttgagt gcctcaggac agagaaactg 2101 gatcattatt ttattccaaa gttcaatcta ttctctcaag aactaattga ccgaaaaagt 2161 aaagaatttc tatcgaagaa aattgaatat gaaagaaata atgggtttcc aatttttgac 2221 aagcttctcg agatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc 2281 actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat 2341 gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct 2401 tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct 2461 gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa 2521 ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa 2581 actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt 2641 ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat 2701 gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta 2761 gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc 2821 aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct 2881 ccaaaatgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat 2941 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 3001 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 3061 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 3121 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 3181 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 3241 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 3301 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 3361 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 3421 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 3481 gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc 3541 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt 3601 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3661 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3721 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc 3781 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 3841 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 3901 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 3961 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 4021 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 4081 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 4141 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt 4201 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 4261 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 4321 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 4381 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 4441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 4501 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 4561 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 4621 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 4681 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 4741 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 4801 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 4861 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 4921 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 4981 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 5041 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 5101 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 5161 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 5221 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 5281 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 5341 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 5401 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 5461 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 5521 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 5581 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 5641 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5701 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 5761 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5821 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 5881 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 5941 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6001 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6061 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6121 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6181 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6241 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6301 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6361 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 6421 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6481 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6541 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6601 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 6661 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6721 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6781 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6841 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 6961 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7021 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7081 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7141 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7201 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7261 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7321 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7381 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7441 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7501 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 7561 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 7621 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 7681 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 7741 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7801 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7861 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 7921 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc 7981 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg 8041 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt 8101 gaccgacaat tgcatgaaga atctgcttag g //