LOCUS       V030911                 5874 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030911
VERSION     V030911
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2021
                     /label="Cd207(NM_144943)"
                     /note="Cd207(NM_144943)"
                     /gene="Cd207"
     CDS             2040..2069
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2088..2111
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2131..2147)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2158..2780
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2929..3517)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3846..3893
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4125..4919)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4954..5311)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5313..5417)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5444..5872
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gccagaggca gagatgaagg aggaggctcc cgaagcgcac ttcacagtgg
     1081 acaaacagaa catctctctc tggcctcgag agcctcctcc caagcaagat ctgtctccag
     1141 ttctaaggaa acctctctgt atctgcgtgg ccttcacctg cctggcattg gtgctggtca
     1201 cctccattgt gcttcaggct gttttctatc ctaggttgat gggcaaaata ttggatgtga
     1261 agagtgatgc ccagatgttg aaaggtcgtg tggacaacat cagcaccctg ggttctgatc
     1321 ttaagactga aagaggtcgt gtggacgatg ctgaggttca gatgcagata gtgaacacca
     1381 ccctcaagag ggtgcgttct cagatcctgt ctttggaaac cagcatgaag atagccaatg
     1441 atcagctcca gatattaaca atgagctggg gagaggttga cagtctcagt gccaaaatcc
     1501 cagaactgaa aagagatctg gataaagcca gcgccttgaa cacaaaggtc caaggactac
     1561 agaacagctt ggagaatgtc aacaagctgc tcaaacaaca gagtgacatt ctggagatgg
     1621 tggctcgagg ctggaagtat ttctcgggga acttctatta cttttcacgc accccaaaga
     1681 cctggtacag cgcagagcag ttctgtattt ctagaaaagc tcacctgacc tcagtgtcct
     1741 cagaatcgga acaaaagttt ctctacaagg cagcagatgg aattccacac tggattggac
     1801 ttaccaaagc agggagcgaa ggggactggt actgggtgga ccagacatca ttcaacaagg
     1861 agcaaagtag gaggttctgg attccaggtg aacccaacaa cgcagggaac aatgagcact
     1921 gtgccaatat cagggtgtct gccctgaagt gctggaacga tggtccctgt gacaatacat
     1981 ttcttttcat ctgcaagagg ccctacgtcc aaacaactga aacgcgtacg cggccgctcg
     2041 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg
     2101 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg
     2161 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     2221 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     2281 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     2341 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     2401 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     2461 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     2521 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     2581 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     2641 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc
     2701 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact
     2761 cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa
     2821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3361 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3601 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3661 tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     3721 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     3781 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     3841 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc
     3901 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc
     3961 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     4021 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     4081 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     4141 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     4201 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     4261 tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     4321 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     4381 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     4441 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     4501 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     4561 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     4621 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     4681 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc
     4741 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     4801 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     4861 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     4921 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
     4981 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     5041 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     5101 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     5161 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     5221 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     5281 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
     5341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5401 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     5461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5581 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5641 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5761 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5821 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt
//
