LOCUS       V034412                11610 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034412
VERSION     V034412
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6290
                     /label="Ccdc88a(NM_176841)"
                     /note="Ccdc88a(NM_176841)"
                     /gene="Ccdc88a"
     CDS             6297..6362
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6386..6974
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7040..7088
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7290..7718
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7732..8061
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8128..8922
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9098..9219
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9268..9284)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9292..9308
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9316..9346)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9361..9382
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9670..10258)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10429..11289)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11290..11394)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga gaacgaaatc ttcactcccc
      781 ttctggagca gttcatgacc agccccttgg tcacttgggt taaaacgttt ggacctctgg
      841 ctgcaggaaa tgggactaac ctggatgagt atgtggcttt agtggatggg gtattcttga
      901 accaggtcat gctccaaatt aatcctaagt cggagagtca acgagtaaat aaaaaagtca
      961 acaatgatgc ttcccttaga attcacaatc tgtccatttt ggtgaaacag ataaaatttt
     1021 attatcagga gactttgcag cagttgatta tgatgccatt gccagatatc ttaatcattg
     1081 gcaaaaatcc cttttctgaa caaggtacag aagaagttaa aaagttgctt ttgctcttac
     1141 ttggttgtgc agttcagtgt cagaaaaagg aagaatttat tgaaaaaatt caaggtttag
     1201 attttgatac gaaagcagca gttgctgcac acattcaaga ggtaactcat aatcaagaaa
     1261 atgtatttga tctgcaatgg atggaagtga cagatatgtc tcaggaagac atagaaccac
     1321 tcttaaagaa tatggtgtca catctaagaa gactgataga tgagagagat gaacattcag
     1381 agactattgt agaactctca gaagagcgag atggtgttca ttttctaccc catgcctctt
     1441 catctgcaca gtcaccctgt ggttctccag gcatgaagcg aacagaaagt cgacagcatc
     1501 tctcagtgga attggctgat gctaaagcca agatccgaag acttaggcag gaattggaag
     1561 aaaagactga gcagttactg gactgtaaac aagaacttga gcaaatagaa gtggaattga
     1621 agaggctgca acaagagaac atgaatttac tttcggatgc tcgatctgct agaatgtacc
     1681 gagatgaatt ggatgccctc cgagagaaag cagtcagagt tgataagctt gaaagtgaac
     1741 tcagtagata taaagagaga ctacatgata ttgaatttta taaggcaaga gttgaggaat
     1801 taaaagaaga taatcaagtt ttattagaaa caaaaactat gctggaagac caactagaag
     1861 gaactcgggc tcgttctgac aaactacatg aattagaaaa agagaattta caactaaagg
     1921 ctaaattgca tgatatggag atggaacgag atatggatag aaagaagatt gaagaattaa
     1981 tggaagaaaa tatgactctg gaaatggcac agaaacaaag tatggatgag tcattacacc
     2041 ttggctggga gctggaacag atatccagaa ctagtgaact tgctgaagca ccacagaagt
     2101 ccttgggcca tgaggtaaat gaattaacat caagtaagtt attaaagttg gaaatggaaa
     2161 accagagtct gacgaaaacc gtagaagagc tccggagcac tgcggactcc gcagcaggca
     2221 gcacttccaa aatcctaaaa gttgaaaagg aaaaccagag actgaataaa aaggttgaaa
     2281 ttcttgaaaa tgaaattatt caagaaaagc aaagtcttca gaattgtcaa aatttaagca
     2341 aggatctaat gaaagagaaa gctcagcttg aaaaaacaat agagacgctc agagagaatt
     2401 cagagaggca gattaaaata ttggagcagg aaaatgaaca tctgaatcaa acagtgtctt
     2461 ccttacggca gcgctcccag attagtgctg aggcgagagt gaaagacatt gaaaaagaaa
     2521 ataaaattct ccatgaatct atcaaagaaa catgtggaaa actaagcaag attgaatttg
     2581 aaaaaagaca aatgaagaag gaactcgaac tgtacaaaga aaaaggagaa agagcagaag
     2641 agcttgagaa tgagttgaat cacctgggga aggagaatga actcctccag aagaagatca
     2701 ccaatctcaa aatcacttgc gaaaagctcg agacactcga acaagaaaac tccgaactcg
     2761 agcgcgagaa tcgcaaattt aaaaagaccc tggacagctt taaaaacctc actttccagc
     2821 tcgaatccct cgagaaggag aacagccaac tcgacgaaga aaacctggag ttgagaaggt
     2881 ctgtggaatc tctgaaatgc gcttctatgc gcatggctca gctccagcta gagaacaagg
     2941 agctggagag tgagaaggag cagctgagga agggcttgga gctcatgaga gcctctttca
     3001 agaagacaga acgcttggaa gttagctacc aggggctaga cacagaaaat caaagactac
     3061 aaaaagctct agaaaatagc aataaaaaaa tccaacagtt agagagtgag ctacaagact
     3121 tagagatgga aaatcaaaca ttacaaaaaa gcctagagga actgaaaata tctagcaaaa
     3181 gactggaaca actagaaaaa gaaaacaagt cattagagca agagacatct caacttgaaa
     3241 aggacaaaaa acaattggag aaggaaaata aacgactccg acaacaagca gaaataaaag
     3301 ataccacatt agaagaaaat aatgtgaaaa ttgggaattt ggaaaaagaa aacaaaactt
     3361 tattcaaaga aattaatgta tataaagagt cctgtgttcg tctaaaagaa ttagaaaaag
     3421 aaaataaaga gcttgtaaaa agagcaacta ttgatattaa aaccttggtt acattacgtg
     3481 aggatttagt cagtgagaaa ttgaagacac aacaaatgaa caatgatctt gagaaattaa
     3541 ctcatgagct tgagaagata ggtttaaata aagagcggct cctacacgat gagcagagca
     3601 ctgatgacag taggtacaaa cttttggaat caaaattaga atccactctt aagaagtccc
     3661 ttgaaataaa agaagaaaaa attgctgctt tagaagctcg gttagaagaa tctacaaatt
     3721 acaatcagca attgcgccac gaacttaaaa cagtgaaaaa aaattatgaa gctctcaaac
     3781 agagacaaga tgaagaaagg atggtacaga gttccattcc agtctctgga gaagatgaca
     3841 agtggggccg agaaagtcaa gaagcgacta gagagctcct gaaagttaaa gacaggttaa
     3901 ttgaagtaga aagaaacaat gctacgctgc aagcagagaa gcaagcactg aaaactcaac
     3961 tgaagcaact tgaaacacaa aataataatt tgcaggctca gattcttgca ctgcagaggc
     4021 agaccgtgtc attgcaggag cagaatacca cgcttcagac acagaatgcc aagcttcagg
     4081 ttgaaaattc tacccttaat tcacaaagta cttcacttat gaaccagaat gcacagcttc
     4141 taatccagca gtcttcctta gaaaatgaaa atgaatctat aatgaaagaa cgagaagact
     4201 tgaaatctct gtatgatgct ctgataaaag atcacgaaaa gctggaactt cttcatgagc
     4261 ggcaagcttc agagtatgag tctctgatct ctaaacatgg caccctgaag tctgctcaca
     4321 aaaatcttga agtggaacat aaagaccttg aagaccgtta caatcagtta ttaaaacaga
     4381 aaggacaatt ggaagatttg gaaaaaatga tcaaaacgga acaggaaaaa atgctgcttg
     4441 aaagcaaaaa ccatgaagtg gtagcttcag agtacaagaa gctttgtggt gaaaatgata
     4501 ggttaaatta tacatatagt cagcttttaa aagagactga aattttacaa atggatcata
     4561 aaaatttgaa aagtgttcta aataattcta aattggaaca aacaagatta gaagctgaat
     4621 tttcaaagct aaaggagcag tatcagcaac tagatatcac atctaccaag ctaaataacc
     4681 agtgtgagtt gctcagccaa cttaaaggaa atttagaaga agaaaatcga catctacttg
     4741 atcagatcca gacactaatg ctacagaaca gaacactgct ggagcaaaat atggaaagca
     4801 aggatctctt tcatgttgaa caaagacagt acattgataa attaaatgaa ttgagacgac
     4861 aaaaagaaaa attagaagag aaaattatgg atcaatacaa attttatgac ccatctcctc
     4921 ctagaaggag aggcaactgg attactctaa aaatgagaaa attgataaag tcaaaaaaag
     4981 atattaatcg ggaacgtcag aaatctctaa cactaacacc cacccgctca gactccagtg
     5041 aaggatttct tcagctgccc catcaagaca gccaagacag ctcttcagta ggatcaaact
     5101 ctttagaaga tggccaaacc ctggggacca agaagagcag caccatgaat gacctggtgc
     5161 agtccatggt cctagcagga ggacagtgga caggtagtac tgaaaatttg gaggtccctg
     5221 atgatatttc aacgggtaaa aggagaaaag aattgggagc tatggccttc tctactacag
     5281 ccatcaactt ttcaactgtc aactcttctg cagccttccg atccaagcag ttggttaata
     5341 ataaagatac tacatccttt gaagacataa gtccacaagg tattagtgat gattctagta
     5401 cgggatcaag agttcatgct tcaagaccag ccagccttga tagtggcaga acatccacta
     5461 gcaatagcaa taataatgct tcactccatg aagtcaaagc aggtgcagtt aatatccaaa
     5521 gcaggccaca aagccacagc agtggagatt tcagtctgct tcatgatcat gagacttggt
     5581 ctagcagtgg tagcagtcca attcagtatt tgaaaagaca gaccaggtca agccctatgc
     5641 tccagcacaa aatatctgaa acaatagaga gtcgagccca tcacaagatg aaagctggct
     5701 ccccaggaag tgaagttgtt actctacaac agtttttgga agaaagcaac aagcttacct
     5761 caatacagtt gaagtcctcg agtcaagaga atcttttaga tgaagtaatg aaaagtttgt
     5821 ctgtgtcttc cgactttctg gggaaagaca agccagttag ctgtaccctg gccaggtcag
     5881 taagtggaaa gactccaggg gacttctatg atagacggac aactaagcct gaatttttga
     5941 gaactggtcc tcaaaagact gaagatgcct acaccatcag ctctgcaggg aaacctacac
     6001 caagcactca aggaaagata aaactagtaa aagaaacctc tgtgtcaaga caatcaaaag
     6061 atagcaatcc ttatgccact ttacctcgtg caagcagtgt gatctctact gctgaaggaa
     6121 caactcgaag gacgagcatc catgattttt tgagcaaaga cagtagactg cccgtgtcag
     6181 tcgattcatc accacctact gctggtagca gcagcactac agcatctaat gtgaacaaag
     6241 tacaagagag cagaaattca aaaagcaggt ctagggagca acaaagctcc ggatccgact
     6301 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
     6361 agtagtgagg gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6421 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6481 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6541 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6601 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6661 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6721 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6781 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6841 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6901 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6961 ggccgcctcc ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg
     7021 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
     7081 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     7141 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     7201 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct
     7261 gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     7321 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7381 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7441 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7501 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7561 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7621 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     7681 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7741 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7801 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7861 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7921 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7981 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     8041 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     8101 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     8161 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     8221 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     8281 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     8341 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     8401 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     8461 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     8521 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     8581 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     8641 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     8701 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     8761 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     8821 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     8881 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     8941 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     9001 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9061 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     9121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9241 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9301 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9361 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9421 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9481 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9841 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     9901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10081 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10321 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10381 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10441 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10501 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10561 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10621 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10681 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10741 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10801 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10861 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10921 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10981 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11041 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11101 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11161 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11221 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11281 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11341 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11401 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg
    11461 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    11521 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    11581 accgacaatt gcatgaagaa tctgcttagg
//
