LOCUS       V013938                 7211 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013938
VERSION     V013938
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1151..2647
                     /gene="CHRNG"
                     /label="Acetylcholine receptor subunit gamma"
                     /note="Acetylcholine receptor subunit gamma from Homo
                     sapiens. Accession#: P07510"
     CDS             2654..2743
                     /label="3xMyc"
                     /note="3 tandem Myc epitope tags"
     polyA_signal    2778..3002
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3048..3476
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3490..3819
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3886..4677
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4854..4987
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5024..5040)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5048..5064)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5072..5102)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5117..5138)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5426..6011)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6185..7042)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7043..7147)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg catgggggcc aggggccgct gctcctcctg ctgctgctgg ctgtctgcct
      961 ggctgggaca caaaggaatc tcagcctggg gagtcccaga gctggggtcc acagcctcag
     1021 gggatggagg gtctgagggg tattggggcc tgccctggac ccagttccct gagtccccac
     1081 ttcacacccc cagggcctcc ccgctctttc cacctccaag ctcctgctag gctcacgcct
     1141 gtctattgca ggggcccagg gccggaacca ggaggagcgc ctgctcgcag acctgatgca
     1201 aaactacgac cccaacctgc ggcccgcgga acgagactcg gatgtggtca atgtcagcct
     1261 gaagctaacc ctcaccaacc tcatctccct gaacgagcga gaggaagccc tcaccaccaa
     1321 tgtctggata gagatgcagt ggtgcgacta tcgcctgcgc tgggatccgc gagactacga
     1381 aggcctgtgg gtgctgaggg tgccgtccac catggtgtgg cggccggata tcgtgctgga
     1441 gaacaacgtg gacggtgtct tcgaggtggc cctctactgc aatgtgctcg tgtcccctga
     1501 cggctgtatc tactggctgc cgcctgccat cttccgttcc gcctgctcta tctcagtcac
     1561 ctacttcccc ttcgactggc agaactgctc ccttatcttc cagtcccaga cttacagcac
     1621 caatgagatt gatctgcagc tgagtcagga agatggccag accatcgagt ggattttcat
     1681 tgaccctgag gccttcacag agaatgggga gtgggccatc cagcaccgac cagccaagat
     1741 gctcctggac ccagcggcgc cagcccagga agcaggccac cagaaggtgg tgttctacct
     1801 gctcatccag cgcaagcccc tcttctacgt catcaacatc atcgccccct gtgtgctcat
     1861 ctcctctgtc gccatcctca tccacttcct tcctgccaag gctgggggcc agaagtgtac
     1921 cgtcgccatc aacgtgctcc tggcccagac tgtcttcctc ttccttgtgg ccaagaaggt
     1981 gcctgaaacc tcccaggcgg tgccactcat cagcaagtac ctgaccttcc tcctggtggt
     2041 gaccatcctc attgtcgtga atgctgtggt tgtgctcaat gtctccttgc ggtctccaca
     2101 cacacactcc atggcccgag gggtccgcaa ggtgttcctg aggctcttgc cccagctgct
     2161 gaggatgcac gttcgcccgc tggccccggc agctgtgcag gacacccagt cccggctaca
     2221 gaatggctcc tcgggatggt cgatcacaac tggggaggag gtggccctct gcctgcctcg
     2281 cagtgaactc ctcttccagc agtggcagcg gcaagggctg gtggcggcag cgctggagaa
     2341 gctagagaaa ggcccggagt tagggctgag ccagttctgt ggcagcctga agcaggctgc
     2401 cccagccatc caggcctgtg tggaagcctg caacctcatt gcctgtgccc ggcaccagca
     2461 gagtcacttt gacaatggga atgaggagtg gttcctggtg ggccgagtgc tggaccgcgt
     2521 ctgcttcctg gccatgctct cgctcttcat ctgtggcaca gctggcatct tcctcatggc
     2581 ccactacaac cgggtgccgg ccctgccatt ccctggagat ccacgcccct acctgccctc
     2641 accagacctc gaggaacaaa aactaatatc agaggaagat ttggaacaga aactaatcag
     2701 tgaagaagat ttagagcaaa agttaatttc cgaggaggac ttatgagggc ccgtttaaac
     2761 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2821 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2881 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2941 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3001 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3061 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3121 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3181 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3241 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3301 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3361 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3421 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3481 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3541 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3601 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3661 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3721 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     3781 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     3841 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     3901 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3961 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4021 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4081 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4141 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4201 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4261 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4321 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4381 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4441 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4501 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4561 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4621 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4681 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     4741 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4801 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     4861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4981 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     5041 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5101 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5161 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5221 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5281 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5341 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5401 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5461 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5521 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5581 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5641 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5701 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5761 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5821 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5881 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5941 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6001 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6061 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6121 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6181 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6241 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6301 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6361 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6421 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6481 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6541 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6601 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6661 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6721 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6781 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6841 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6901 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6961 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7021 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7081 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7141 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     7201 tcccctatgg t
//
