LOCUS       V036937                 9924 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036937
VERSION     V036937
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..4604
                     /label="CFAP57(NM_001195831)"
                     /note="CFAP57(NM_001195831)"
                     /gene="CFAP57"
     CDS             4611..4676
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    4700..5288
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5354..5402
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5604..6032
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6046..6375
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6442..7236
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7412..7533
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7582..7598)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7606..7622
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7630..7660)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7675..7696
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7984..8572)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8743..9603)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9604..9708)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgtc agccgtggta gctcagacgc
      781 tgcatgtttt tggtcttcga tcccacgtgg ccaacaatat cttctacttc gatgaacaga
      841 tcattatatt tccttcagga aatcactgtg tgaagtacaa tgtggatcag aaatggcaaa
      901 aattcattcc aggctcagag aagagtcagg gcatgttggc cttgtccatc agtcccaatc
      961 ggcggtacct cgctatctct gagactgtgc aagaaaaacc tgccatcacc atttatgaat
     1021 tgtcatccat cccttgccgg aagcgcaaag ttcttaataa ttttgacttc caagttcaga
     1081 aatttattag catggctttt tctccagact ccaaatacct attggctcag acgtcacctc
     1141 cagagtcaaa tcttgtctac tggctgtggg aaaaacagaa agtaatggcc attgttagaa
     1201 tcgacactca gaacaaccct gtctaccagg tgagcttcag tccacaggat aacactcagg
     1261 tgtgtgtcac tggaaatggg atgtttaagc ttctccgttt tgctgaggga accctgaagc
     1321 aaaccagctt tcagagggga gaaccccaaa actatctagc tcacacctgg gtggctgatg
     1381 acaagattgt cgttggcact gacacaggca aactcttcct ctttgaatct ggagatcagc
     1441 gttgggagac cagcataatg gtcaaggaac ctaccaatgg ctcaaagagc ctggatgtca
     1501 ttcaggaatc agagagcctg attgaatttc caccagtcag ttctccactc ccttcctatg
     1561 aacagatggt ggcggccagt agccatagcc agatgtccat gccccaggtg tttgccattg
     1621 cagcctattc aaagggattt gcctgttctg ctgggccagg gagagttctg ctgtttgaga
     1681 agatggaaga aaaggatttt taccgtgaga gcagagaaat caggattcct gtggacccgc
     1741 agagcaatga tccaagtcag tctgacaaac aggacgttct ctgcctgtgc ttcagcccct
     1801 cagaggaaac tctggttgcc agcaccagta agaaccaact ctacagcatc accatgtccc
     1861 tgacagagat cagcaagggg gagcctgctc actttgagta tttgatgtat ccattgcact
     1921 cagcacccat caccggtcta gctacctgca tccgcaaacc ccttatagcc acctgttctc
     1981 tggatcgatc catccgcctt tggaattatg aaacaaacac cctggaacta tttaaggaat
     2041 accaagaaga ggcatattcc atcagccttc atccatctgg acacttcatt gtagtagggt
     2101 ttgctgacaa actacgcctc atgaatctac tcattgatga tatacgttct ttcaaagaat
     2161 actctgttag aggatgcgga gagtgttcct ttagcaatgg aggtcacctg tttgctgcag
     2221 tcaatggaaa tgtgattcac gtttacacca ccacgagcct agagaacatc tcaagcctga
     2281 aaggacacac agggaagatt cgctcaattg tgtggaatgc agatgatagc aaactgattt
     2341 ctggtggcac agatggtgct gtgtatgaat ggaatctgtc cacaggaaag agagagacag
     2401 aatgcgtgct caagtcttgc agctacaact gtgttactgt ctcccccgat gccaaaatta
     2461 tctttgctgt tggatcagac cacaccctca aggagattgc agattccttg atccttcgag
     2521 agatatcggc gtttgatgtc acctacaccg ccattgtcat ctcgcattct ggacgcatga
     2581 tgtttgtggg cacctcggtg ggaaccattc gtgccatgaa gtaccctctg cctctgcaga
     2641 aggaattcaa tgagtaccag gcccatgccg gtcctatcac caagatgttg cttacctttg
     2701 atgatcagtt cctgctgact gctgctgagg atggctgcct gttcacctgg aaggtctttg
     2761 ataaggatgg ccggggaatc aagcgagaga gggaggtggg ctttgccgaa gaggtgcttg
     2821 tgactaaaac agacatggaa gaaaaggctc aggttatgtt ggagctaaag actcgtgtgg
     2881 aggaattaaa aatggagaat gagtatcaac tccgactaaa ggacatgaac tattctgaga
     2941 agattaagga gctaacagac aagttcatcc aggaaatgga gtccttgaaa acaaaaaacc
     3001 aggtcttaag aacagaaaaa gagaagcagg atgtttatca ccatgagcac atagaagacc
     3061 tcctagacaa gcaaagccgg gaactgcagg acatggaatg ttgcaacaac caaaagttgc
     3121 ttctagaata tgagaagtac caggagctgc agctcaagtc ccagaggatg caggaagagt
     3181 atgaaaaaca gctccgggat aacgatgaga ccaagagcca ggccctggag gagctgactg
     3241 agttttacga ggcaaaactg caggagaaaa ccacccttct ggaagaggca caggaagacg
     3301 tcaggcagca gctgcgggag tttgaagaga ccaagaagca gattgaggaa gatgaagacc
     3361 gagaaatcca agatatcaaa accaagtatg agaaaaagct tcgggatgaa aaggaatcaa
     3421 acctgcggct caagggagaa acaggcatca tgaggaagaa gttcagcagc ctacagaagg
     3481 agattgaaga acgaaccaat gacatcgaga ccctaaaagg agagcagatg aagctgcaag
     3541 gagtcattaa gtctctggag aaggacatcc aaggcctcaa gcgagagatc caggaaagag
     3601 acgagactat tcaagacaag gagaagcgaa tttatgatct gaaaaagaaa aatcaagaac
     3661 tagggaaatt caagtttgtg cttgactaca aaataaagga gctgaagaag caaatagaac
     3721 ctcgagagaa tgagatcagg gtgatgaagg aacagattca ggagaatgac cccattctaa
     3781 agtggcccca gagggtcgag attcacttat acaacttcag aaagcaggtg aggaaggtgc
     3841 ttctctgggg aaccctgcct cagatggaag ctgaactgga gaatttccat aagcagaaca
     3901 ctcaactgga gctgaacatc acagaattgt ggcagaaact gagagccacc gatcaggaga
     3961 tgcgcagaga gagacagaag gagcgagact tggaagcgct ggtcaaaagg tttaaaacag
     4021 acctccacaa ctgcgtagcc tatattcagg aaccgcggct gctgaaggag aaggttcgag
     4081 gtctctttga gaagtacgtg cagcgagcag acatggtgga gatcgcaggg ctgaacacag
     4141 acctgcagca ggagtacacc cggcagcggg agcacctgga gaggaacctg gccactctca
     4201 agaagaaggt ggtcaaggag ggcgagctgc accgcacaga ctacgtccgc atcatgcagg
     4261 aaaatgtctc tctgatcaag gaaattaatg agctccgcag ggagctgaag ttcactcggt
     4321 cccaagtcta tgaccttgaa gcagctctga aactgaccaa gaaagtccga ccacaagaag
     4381 tttcagagac agaacccagc agggacatgc tcagcacagc tcccaccgca aggttgaatg
     4441 agcaagaaga aactgggagg atcattgaaa tgcagcgcct agaaatccag cgcctcagag
     4501 accagatcca agagcaagag caggtcacag ggttccacac cctcgctgga gttcggcttc
     4561 cttccctctc caactccgag gtagacttag aggtgaagac caacggatcc gactacaaag
     4621 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt
     4681 gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4741 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4801 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4861 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4921 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4981 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5041 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5101 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5161 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5221 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5281 ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
     5341 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     5401 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5461 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5521 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
     5581 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5761 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5941 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     6001 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6121 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6301 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6361 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     6421 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6481 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6541 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6601 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6661 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6721 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6781 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6841 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6901 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6961 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     7021 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7081 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7141 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7201 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat
     7261 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     7321 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7381 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7501 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7561 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7681 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7741 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8161 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8401 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8521 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8581 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8641 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8701 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8821 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8881 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9421 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9481 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9541 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9601 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9661 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9721 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc
     9781 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     9841 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     9901 aattgcatga agaatctgct tagg
//
