LOCUS       V032299                12744 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032299
VERSION     V032299
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             750..7424
                     /label="CAD(NM_004341)"
                     /note="CAD(NM_004341)"
                     /gene="CAD"
     CDS             7431..7496
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    7520..8108
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8174..8222
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8424..8852
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8866..9195
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9262..10056
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10232..10353
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10402..10418)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10426..10442
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10450..10480)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10495..10516
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10804..11392)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11563..12423)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12424..12528)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattca tggcggccct agtgttggag gacgggtcgg
      781 tcctgcgggg ccagcccttt ggggccgccg tgtcgactgc cggggaagtg gtgtttcaaa
      841 ccggcatggt cggctacccc gaggccctca ctgatccctc ctacaaggca cagatcttag
      901 tgctcaccta tcctctgatc ggcaactatg gcatcccccc agatgaaatg gatgagttcg
      961 gtctctgcaa gtggtttgaa tcctcgggca tccacgtagc agcactggta gtgggagagt
     1021 gctgtcctac tcccagccac tggagtgcca cccgcaccct gcatgagtgg ctgcagcagc
     1081 atggcatccc tggcttgcaa ggagtagaca ctcgggagct gaccaagaag ttgcgggaac
     1141 aggggtctct gctggggaag ctggtccaga atggaacaga accttcatcc ctgccattct
     1201 tggaccccaa tgcccgcccc ctggtaccag aggtctccat taagactcca cgggtattca
     1261 atacaggggg tgcccctcgg atccttgctt tggactgtgg cctcaagtat aatcagatcc
     1321 gatgcctctg ccagcgtggg gctgaggtca ctgtggtacc ctgggaccat gcactagaca
     1381 gccaagagta tgagggtctc ttcttaagta atgggcctgg tgaccctgcc tcctatccca
     1441 gtgtcgtatc cacactgagc cgtgttttat ctgagcctaa tccccgacct gtctttggga
     1501 tctgcctggg acaccagcta ttggccttag ccattggggc caagacttac aagatgagat
     1561 atgggaaccg aggccataac cagccctgct tgttggtggg ctctgggcgc tgctttctga
     1621 catcccagaa ccatgggttt gctgtggaga cagactcact gccagcagac tgggctcctc
     1681 tcttcaccaa cgccaatgat ggttccaatg aaggcattgt gcacaacagc ttgcctttct
     1741 tcagtgtcca gtttcaccca gagcaccaag ctggcccttc agatatggaa ctgcttttcg
     1801 atatctttct ggaaactgtg aaagaggcca cagctgggaa ccctgggggc cagacagtta
     1861 gagagcggct gactgagcgc ctctgtcccc ctgggattcc cactcccggc tctggacttc
     1921 caccaccacg aaaggttctg atcctgggct cagggggcct ctccattggc caagctggag
     1981 aatttgacta ctcgggctct caggcaatta aggccctgaa ggaggaaaac atccagacgt
     2041 tgctgatcaa ccccaatatt gccacagtgc agacctccca ggggctggcc gacaaggtct
     2101 attttcttcc cataacacct cattatgtaa cccaggtgat acgtaatgaa cgccccgatg
     2161 gtgtgttact gacttttggg ggccagactg ctctgaactg tggtgtggag ctgaccaagg
     2221 ccggggtgct ggctcggtat ggggtccggg tcctgggcac accagtggag accattgagc
     2281 tgaccgagga tcgacgggcc tttgctgcca gaatggcaga gatcggagag catgtggccc
     2341 cgagcgaggc agcaaattct cttgaacagg cccaggcagc cgctgaacgg ctggggtacc
     2401 ctgtgctagt gcgtgcagcc tttgccctgg gtggcctggg ctctggcttt gcctctaaca
     2461 gggaggagct ctctgctctc gtggccccag cttttgccca taccagccaa gtgctagtag
     2521 acaagtctct gaagggatgg aaggagattg agtacgaggt ggtgagagac gcctatggca
     2581 actgtgtcac ggtgtgtaac atggagaact tggacccact gggcatccac actggtgagt
     2641 ccatagtggt ggcccctagc cagacactga atgacaggga gtatcagctc ctgaggcaga
     2701 cagctatcaa ggtgacccag cacctgggaa ttgttgggga gtgcaatgtg cagtatgcct
     2761 tgaaccctga gtctgagcag tattacatca ttgaagtgaa tgccaggctc tctcgcagct
     2821 ctgccctggc cagtaaggcc acaggttatc cactggctta tgtggcagcc aagctagcat
     2881 tgggcatccc tttgcctgag ctcaggaact ctgtgacagg gggtacagca gcctttgaac
     2941 ccagcgtgga ttattgtgtg gtgaagattc ctcgatggga ccttagcaag ttcctgcgag
     3001 tcagcacaaa gattgggagc tgcatgaaga gcgttggtga agtcatgggc attgggcgtt
     3061 catttgagga ggccttccag aaggccctgc gcatggtgga tgagaactgt gtgggctttg
     3121 atcacacagt gaaaccagtc agcgatatgg agttggagac tccaacagat aagcggattt
     3181 ttgtggtggc agctgctttg tgggctggtt attcagtgga ccgcctgtat gagctcacac
     3241 gcatcgaccg ctggttcctg caccgaatga agcgtatcat cgcacatgcc cagctgctag
     3301 aacaacaccg tggacagcct ttgccgccag acctgctgca acaggccaag tgtcttggct
     3361 tctcagacaa acagattgcc cttgcagttc tgagcacaga gctggctgtt cgcaagctgc
     3421 gtcaggaact ggggatctgt ccagcagtga aacagattga cacagttgca gctgagtggc
     3481 cagcccagac aaattaccta tacctaacgt attggggcac cacccatgac ctcacctttc
     3541 gaacacctca tgtcctagtc cttggctctg gcgtctaccg tattggctct agcgttgaat
     3601 ttgactggtg tgctgtaggc tgcatccagc agctccgaaa gatgggatat aagaccatca
     3661 tggtgaacta taacccagag acagtcagca ccgactatga catgtgtgat cgactctact
     3721 ttgatgagat ctcttttgag gtggtgatgg acatctatga gctcgagaac cctgaaggtg
     3781 tgatcctatc catgggtgga cagctgccca acaacatggc catggcgttg catcggcagc
     3841 agtgccgggt gctgggcacc tcccctgaag ccattgactc ggctgagaac cgtttcaagt
     3901 tttcccggct ccttgacacc attggtatca gccagcctca gtggagggag ctcagtgacc
     3961 tcgagtctgc tcgccaattc tgccagaccg tggggtaccc ctgtgtggtg cgcccctcct
     4021 atgtgctgag cggtgctgct atgaatgtgg cctacacgga tggagacctg gagcgcttcc
     4081 tgagcagcgc agcagccgtc tccaaagagc atcccgtggt catctccaag ttcatccagg
     4141 aggctaagga gattgacgtg gatgccgtgg cctctgatgg tgtggtggca gccatcgcca
     4201 tctctgagca tgtggagaat gcaggtgtgc attcaggtga tgcgacgctg gtgacccccc
     4261 cacaagatat cactgccaaa accctggagc ggatcaaagc cattgtgcat gctgtgggcc
     4321 aggagctaca ggtcacagga cccttcaatc tgcagctcat tgccaaggat gaccagctga
     4381 aagttattga atgcaacgta cgtgtctctc gctccttccc cttcgtttcc aagacactgg
     4441 gtgtggacct agtagccttg gccacgcggg tcatcatggg ggaagaagtg gaacctgtgg
     4501 ggctaatgac tggttctgga gtcgtgggag taaaggtgcc tcagttctcc ttctcccgct
     4561 tggcgggtgc tgacgtggtg ttgggtgtgg aaatgaccag tactggggag gtggccggct
     4621 ttggggagag ccgctgtgag gcatacctca aggccatgct aagcactggc tttaagatcc
     4681 ccaagaagaa tatcctgctg accattggca gctataagaa caaaagcgag ctgctcccaa
     4741 ctgtgcggct actggagagc ctgggctaca gcctctatgc cagtctcggc acagctgact
     4801 tctacactga gcatggcgtc aaggtaacag ctgtggactg gcactttgag gaggctgtgg
     4861 atggtgagtg cccaccacag cggagcatcc tggagcagct agctgagaaa aactttgagc
     4921 tggtgattaa cctgtcaatg cgtggagctg ggggccggcg tctctcttcc tttgtcacca
     4981 agggctaccg cacccgacgc ttggccgctg acttctccgt gcccctaatc atcgatatca
     5041 agtgcaccaa actctttgtg gaggccctag gccagatcgg gccagcccct cctttgaagg
     5101 tgcatgttga ctgtatgacc tcccaaaagc ttgtgcgact gccgggattg attgatgtcc
     5161 atgtgcacct gcgggaacca ggtgggacac ataaggagga ctttgcttca ggcacagccg
     5221 ctgccctggc tgggggtatc accatggtgt gtgccatgcc taatacccgg ccccccatca
     5281 ttgacgcccc tgctctggcc ctggcccaga agctggcaga ggctggcgcc cggtgcgact
     5341 ttgcgctatt ccttggggcc tcgtctgaaa atgcaggaac cttgggcacc gtggccgggt
     5401 ctgcagccgg gctgaagctt tacctcaatg agaccttctc tgagctgcgg ctggacagcg
     5461 tggtccagtg gatggagcat ttcgagacat ggccctccca cctccccatt gtggctcacg
     5521 cagagcagca aaccgtggct gctgtcctca tggtggctca gctcactcag cgctcagtgc
     5581 acatatgtca cgtggcacgg aaggaggaga tcctgctaat taaagctgca aaggcacggg
     5641 gcttgccagt gacctgcgag gtggctcccc accacctgtt cctaagccat gatgacctgg
     5701 agcgcctggg gcctgggaag ggggaggtcc ggcctgagct tggctcccgc caggatgtgg
     5761 aagccctgtg ggagaacatg gctgtcatcg actgctttgc ctcagaccat gctccccata
     5821 ccttggagga gaagtgtggg tccaggcccc cacctgggtt cccagggtta gagaccatgc
     5881 tgccactact cctgacggct gtaagcgagg gccggctcag cctggacgac ctgctgcagc
     5941 gattgcacca caatcctcgg cgcatctttc acctgccccc gcaggaggac acctatgtgg
     6001 aggtggatct ggagcatgag tggacaattc ccagccacat gcccttctcc aaggcccact
     6061 ggacaccttt tgaagggcag aaagtgaagg gcaccgtccg ccgtgtggtc ctgcgagggg
     6121 aggttgccta tatcgatggg caggttctgg tacccccggg ctatggacag gatgtacgga
     6181 agtggccaca gggggctgtt cctcagctcc caccctcagc ccctgccact agtgagatga
     6241 ccacgacacc tgaaagaccc cgccgtggca tcccagggct tcctgatggc cgcttccatc
     6301 tgccgccccg aatccatcga gcctccgacc caggtttgcc agctgaggag ccaaaggaga
     6361 agtcctctcg gaaggtagcc gagccagagc tgatgggaac ccctgatggc acctgctacc
     6421 ctccaccacc agtaccgaga caggcatctc cccagaacct ggggacccct ggcttgctgc
     6481 acccccagac ctcacccctg ctgcactcat tagtgggcca acatatcctg tccgtccagc
     6541 agttcaccaa ggatcagatg tctcacctgt tcaatgtggc acacacactg cgtatgatgg
     6601 tgcagaagga gcggagcctc gacatcctga aggggaaggt catggcctcc atgttctatg
     6661 aagtgagcac acggaccagc agctcctttg cagcagccat ggcccggctg ggaggtgctg
     6721 tgctcagctt ctcggaagcc acatcgtccg tccagaaggg cgaatccctg gctgactccg
     6781 tgcagaccat gagctgctat gccgacgtcg tcgtgctccg gcacccccag cctggagcag
     6841 tggagctggc cgccaagcac tgccggaggc cagtgatcaa tgctggggat ggggtcggag
     6901 agcaccccac ccaggccctg ctggacatct tcaccatccg tgaggagctg ggaactgtca
     6961 atggcatgac gatcacgatg gtgggtgacc tgaagcacgg acgcacagta cattccctgg
     7021 cctgcctgct cacccagtat cgtgtcagcc tgcgctacgt ggcacctccc agcctgcgca
     7081 tgccacccac tgtgcgggcc ttcgtggcct cccgcggcac caagcaggag gaattcgaga
     7141 gcattgagga ggcgctgcct gacactgatg tgctctacat gactcgaatc cagaaggaac
     7201 gatttggctc tacccaggag tacgaagctt gctttggtca gttcatcctc actccccaca
     7261 tcatgacccg ggccaagaag aagatggtgg tgatgcaccc gatgccccgt gtcaacgaga
     7321 taagcgtgga agtggactcg gatccccgcg cagcctactt ccgccaggct gagaacggca
     7381 tgtacatccg catggctctg ttagccaccg tgctgggccg tttcggatcc gactacaaag
     7441 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt
     7501 gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7681 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7741 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7801 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7861 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7921 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7981 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8041 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8101 ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
     8161 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     8221 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     8281 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     8341 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
     8401 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8461 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8521 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8581 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8641 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8701 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8761 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     8821 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     8881 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8941 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9001 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9061 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9121 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9181 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     9241 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     9301 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     9361 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     9421 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     9481 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     9541 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     9601 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     9661 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     9721 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     9781 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     9841 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     9901 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     9961 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    10021 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat
    10081 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
    10141 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    10201 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    10261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10321 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    10381 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    10441 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    10501 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    10561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    10621 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10981 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11221 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    11281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    11521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    11581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    11641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    11701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    11761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    11821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    12001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    12061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    12121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    12181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    12241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    12301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    12361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    12481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    12541 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc
    12601 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    12661 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
    12721 aattgcatga agaatctgct tagg
//
