LOCUS       V028523                13865 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028523
VERSION     V028523
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             775..7791
                     /label="CACNA1B(NM_000718)"
                     /note="CACNA1B(NM_000718)"
                     /gene="CACNA1B"
     CDS             7807..7863
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             7864..8574
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    8641..9229
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9295..9343
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9545..9973
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9987..10316
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10383..11177
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11353..11474
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11523..11539)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11547..11563
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11571..11601)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11616..11637
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11925..12513)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12684..13544)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13545..13649)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccgc caccatggtc
      781 cgcttcgggg acgagctggg cggccgctat gggggccccg gcggcggaga gcgggcccgg
      841 ggcggcgggg ccggcggggc ggggggcccg ggtcccgggg ggctgcagcc cggccagcgg
      901 gtcctctaca agcaatcgat cgcgcagcgc gcgcggacca tggcgctgta caaccccatc
      961 ccggtcaagc agaactgctt caccgtcaac cgctcgctct tcgtcttcag cgaggacaac
     1021 gtcgtccgca aatacgcgaa gcgcatcacc gagtggcctc cattcgagta tatgatcctg
     1081 gccaccatca tcgccaactg catcgtgctg gccctggagc agcacctccc tgatggggac
     1141 aaaacgccca tgtccgagcg gctggacgac acggagccct atttcatcgg gatcttttgc
     1201 ttcgaggcag ggatcaaaat catcgctctg ggctttgtct tccacaaggg ctcttacctg
     1261 cggaacggct ggaacgtcat ggacttcgtg gtcgtcctca cagggatcct tgccacggct
     1321 ggaactgact tcgacctgcg aacactgagg gctgtgcgtg tgctgaggcc cctgaagctg
     1381 gtgtctggga ttccaagttt gcaggtggtg ctcaagtcca tcatgaaggc catggttcca
     1441 ctcctgcaga ttgggctgct tctcttcttt gccatcctca tgtttgccat cattggcctg
     1501 gagttctaca tgggcaagtt ccacaaggcc tgtttcccca acagcacaga tgcggagccc
     1561 gtgggtgact tcccctgtgg caaggaggcc ccagcccggc tgtgcgaggg cgacactgag
     1621 tgccgggagt actggccagg acccaacttt ggcatcacca actttgacaa tatcctgttt
     1681 gccatcttga cggtgttcca gtgcatcacc atggagggct ggactgacat cctctataat
     1741 acaaacgatg cggccggcaa cacctggaac tggctctact tcatccctct catcatcatc
     1801 ggctccttct tcatgctcaa cctggtgctg ggcgtgctct cgggggagtt tgccaaggag
     1861 cgagagaggg tggagaaccg ccgcgccttc ctgaagctgc gccggcagca gcagatcgag
     1921 cgagagctca acgggtacct ggagtggatc ttcaaggcgg aggaagtcat gctggccgag
     1981 gaggacagga atgcagagga gaagtcccct ttggacgtgc tgaagagagc ggccaccaag
     2041 aagagcagaa atgacctgat ccacgcagag gagggagagg accggtttgc agatctctgt
     2101 gctgttggat cccccttcgc ccgcgccagc ctcaagagcg ggaagacaga gagctcgtca
     2161 tacttccgga ggaaggagaa gatgttccgg ttttttatcc ggcgcatggt gaaggctcag
     2221 agcttctact gggtggtgct gtgcgtggtg gccctgaaca cactgtgtgt ggccatggtg
     2281 cattacaacc agccgcggcg gcttaccacg accctgtatt ttgcagagtt tgttttcctg
     2341 ggtctcttcc tcacagagat gtccctgaag atgtatggcc tggggcccag aagctacttc
     2401 cggtcctcct tcaactgctt cgactttggg gtcatcgtgg ggagcgtctt tgaagtggtc
     2461 tgggcggcca tcaagccggg aagctccttt gggatcagtg tgctgcgggc cctccgcctg
     2521 ctgaggatct tcaaagtcac gaagtactgg agctccctgc ggaacctggt ggtgtccctg
     2581 ctgaactcca tgaagtccat catcagcctg ctcttcttgc tcttcctgtt cattgtggtc
     2641 ttcgccctgc tggggatgca gctgtttggg ggacagttca acttccagga tgagactccc
     2701 acaaccaact tcgacacctt ccctgccgcc atcctcactg tcttccagat cctgacggga
     2761 gaggactgga atgcagtgat gtatcacggg atcgaatcgc aaggcggcgt cagcaaaggc
     2821 atgttctcgt ccttttactt cattgtcctg acactgttcg gaaactacac tctgctgaat
     2881 gtctttctgg ccatcgctgt ggacaacctg gccaacgccc aagagctgac caaggatgaa
     2941 gaggagatgg aagaagcagc caatcagaag cttgctctgc aaaaggccaa agaagtggct
     3001 gaagtcagcc ccatgtctgc cgcgaacatc tccatcgccg ccaggcagca gaactcggcc
     3061 aaggcgcgct cggtgtggga gcagcgggcc agccagctac ggctgcagaa cctgcgggcc
     3121 agctgcgagg cgctgtacag cgagatggac cccgaggagc ggctgcgctt cgccactacg
     3181 cgccacctgc ggcccgacat gaagacgcac ctggaccggc cgctggtggt ggagctgggc
     3241 cgcgacggcg cgcgggggcc cgtgggaggc aaagcccgac ctgaggctgc ggaggccccc
     3301 gagggcgtcg accctccgcg caggcaccac cggcaccgcg acaaggacaa gacccccgcg
     3361 gcgggggacc aggaccgagc agaggccccg aaggcggaga gcggggagcc cggtgcccgg
     3421 gaggagcggc cgcggccgca ccgcagccac agcaaggagg ccgcggggcc cccggaggcg
     3481 cggagcgagc gcggccgagg cccaggcccc gagggcggcc ggcggcacca ccggcgcggc
     3541 tccccggagg aggcggccga gcgggagccc cgacgccacc gcgcgcaccg gcaccaggat
     3601 ccgagcaagg agtgcgccgg cgccaagggc gagcggcgcg cgcggcaccg cggcggcccc
     3661 cgagcggggc cccgggaggc ggagagcggg gaggagccgg cgcggcggca ccgggcccgg
     3721 cacaaggcgc agcctgctca cgaggctgtg gagaaggaga ccacggagaa ggaggccacg
     3781 gagaaggagg ctgagatagt ggaagccgac aaggaaaagg agctccggaa ccaccagccc
     3841 cgggagccac actgtgacct ggagaccagt gggactgtga ctgtgggtcc catgcacaca
     3901 ctgcccagca cctgtctcca gaaggtggag gaacagccag aggatgcaga caatcagcgg
     3961 aacgtcactc gcatgggcag tcagccccca gacccgaaca ctattgtaca tatcccagtg
     4021 atgctgacgg gccctcttgg ggaagccacg gtcgttccca gtggtaacgt ggacctggaa
     4081 agccaagcag aggggaagaa ggaggtggaa gcggatgacg tgatgaggag cggcccccgg
     4141 cctatcgtcc catacagctc catgttctgt ttaagcccca ccaacctgct ccgccgcttc
     4201 tgccactaca tcgtgaccat gaggtacttc gaggtggtca ttctcgtggt catcgccttg
     4261 agcagcatcg ccctggctgc tgaggaccca gtgcgcacag actcgcccag gaacaacgct
     4321 ctgaaatacc tggattacat tttcactggt gtctttacct ttgagatggt gataaagatg
     4381 atcgacttgg gactgctgct tcaccctgga gcctatttcc gggacttgtg gaacattctg
     4441 gacttcattg tggtcagtgg cgccctggtg gcgtttgctt tctcaggatc caaagggaaa
     4501 gacatcaata ccatcaagtc tctgagagtc cttcgtgtcc tgcggcccct caagaccatc
     4561 aaacggctgc ccaagctcaa ggctgtgttt gactgtgtgg tgaactccct gaagaatgtc
     4621 ctcaacatct tgattgtcta catgctcttc atgttcatat ttgccgtcat tgcggtgcag
     4681 ctcttcaaag ggaagttttt ctactgcaca gatgaatcca aggagctgga gagggactgc
     4741 aggggtcagt atttggatta tgagaaggag gaagtggaag ctcagcccag gcagtggaag
     4801 aaatacgact ttcactacga caatgtgctc tgggctctgc tgacgctgtt cacagtgtcc
     4861 acgggagaag gctggcccat ggtgctgaaa cactccgtgg atgccaccta tgaggagcag
     4921 ggtccaagcc ctgggtaccg catggagctg tccatcttct acgtggtcta ctttgtggtc
     4981 tttcccttct tcttcgtcaa catctttgtg gctttgatca tcatcacctt ccaggagcag
     5041 ggggacaagg tgatgtctga atgcagcctg gagaagaacg agagggcttg cattgacttc
     5101 gccatcagcg ccaaacccct gacacggtac atgccccaaa accggcagtc gttccagtat
     5161 aagacgtgga catttgtggt ctccccgccc tttgaatact tcatcatggc catgatagcc
     5221 ctcaacactg tggtgctgat gatgaagttc tatgatgcac cctatgagta cgagctgatg
     5281 ctgaaatgcc tgaacatcgt gttcacatcc atgttctcca tggaatgcgt gctgaagatc
     5341 atcgcctttg gggtgctgaa ctatttcaga gatgcctgga atgtctttga ctttgtcact
     5401 gtgttgggaa gtattactga tattttagta acagagattg cggaaacgaa caatttcatc
     5461 aacctcagct tcctccgcct ctttcgagct gcgcggctga tcaagctgct ccgccagggc
     5521 tacaccatcc gcatcctgct gtggaccttt gtccagtcct tcaaggccct gccctacgtg
     5581 tgtctgctca ttgccatgct gttcttcatc tacgccatca tcggcatgca ggtgtttggg
     5641 aatattgccc tggatgatga caccagcatc aaccgccaca acaacttccg gacgtttttg
     5701 caagccctga tgctgctgtt caggagcgcc acgggggagg cctggcacga gatcatgctg
     5761 tcctgcctga gcaaccaggc ctgtgatgag caggccaatg ccaccgagtg tggaagtgac
     5821 tttgcctact tctacttcgt ctccttcatc ttcctgtgct cctttctgat gttgaacctc
     5881 tttgtggctg tgatcatgga caattttgag tacctcacgc gggactcttc catcctaggt
     5941 cctcaccact tggatgagtt catccgggtc tgggctgaat acgacccggc tgcgtgtggg
     6001 cgcatcagtt acaatgacat gtttgagatg ctgaaacaca tgtccccgcc tctggggctg
     6061 gggaagaaat gccctgctcg agttgcttac aagcgcctgg ttcgcatgaa catgcccatc
     6121 tccaacgagg acatgactgt tcacttcacg tccacgctga tggccctcat ccggacggca
     6181 ctggagatca agctggcccc agctgggaca aagcagcatc agtgtgacgc ggagttgagg
     6241 aaggagattt ccgttgtgtg ggccaatctg ccccagaaga ctttggactt gctggtacca
     6301 ccccataagc ctgatgagat gacagtgggg aaggtttatg cagctctgat gatattcgac
     6361 ttctacaagc agaacaaaac caccagagac cagatgcagc aggctcctgg aggcctctcc
     6421 cagatgggtc ctgtgtccct gttccaccct ctgaaggcca ccctggagca gacacagccg
     6481 gctgtgctcc gaggagcccg ggttttcctt cgacagaaga gttccacctc cctcagcaat
     6541 ggcggggcca tacaaaacca agagagtggc atcaaagagt ctgtctcctg gggcactcaa
     6601 aggacccagg atgcacccca tgaggccagg ccacccctgg agcgtggcca ctccacagag
     6661 atccctgtgg ggcggtcagg agcactggct gtggacgttc agatgcagag cataacccgg
     6721 aggggccctg atggggagcc ccagcctggg ctggagagcc agggtcgagc ggcctccatg
     6781 ccccgccttg cggccgagac tcagcccgtc acagatgcca gccccatgaa gcgctccatc
     6841 tccacgctgg cccagcggcc ccgtgggact catctttgca gcaccacccc ggaccgccca
     6901 ccccctagcc aggcgtcgtc gcaccaccac caccaccgct gccaccgccg cagggacagg
     6961 aagcagaggt ccctggagaa ggggcccagc ctgtctgccg atatggatgg cgcaccaagc
     7021 agtgctgtgg ggccggggct gcccccggga gaggggccta caggctgccg gcgggaacga
     7081 gagcgccggc aggagcgggg ccggtcccag gagcggaggc agccctcatc ctcctcctcg
     7141 gagaagcagc gcttctactc ctgcgaccgc tttgggggcc gtgagccccc gaagcccaag
     7201 ccctccctca gcagccaccc aacgtcgcca acagctggcc aggagccggg accccaccca
     7261 cagggcagtg gttccgtgaa tgggagcccc ttgctgtcaa catctggtgc tagcaccccc
     7321 ggccgcggtg ggcggaggca gctcccccag acgcccctga ctccccgccc cagcatcacc
     7381 tacaagacgg ccaactcctc acccatccac ttcgccgggg ctcagaccag cctccctgcc
     7441 ttctccccag gccggctcag ccgtgggctt tccgaacaca acgccctgct gcagagagac
     7501 cccctcagcc agcccctggc ccctggctct cgaattggct ctgaccctta cctggggcag
     7561 cgtctggaca gtgaggcctc tgtccacgcc ctgcctgagg acactctcac tttcgaggag
     7621 gctgtggcca ccaactcggg ccgctcctcc aggacttcct acgtgtcctc cctgacctcc
     7681 cagtctcacc ctctccgccg cgtgcccaac ggttaccact gcaccctggg actcagctcg
     7741 ggtggccgag cacggcacag ctaccaccac cctgaccaag accactggtg cgaattcgga
     7801 agcggagcta ctaacttcag cctgctgaag caggctggcg acgtggagga gaaccctgga
     7861 cctatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     7921 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     7981 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     8041 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     8101 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     8161 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     8221 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     8281 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     8341 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     8401 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     8461 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     8521 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaactcgag
     8581 tctagagggc cccttaaggg ttcgatccct accggttagt aatgagtttg atatctcgac
     8641 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8701 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8761 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8821 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8881 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8941 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9001 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     9061 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9121 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9181 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaaacggg
     9241 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     9301 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
     9361 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     9421 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     9481 aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat
     9541 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9601 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9661 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     9721 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9781 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9841 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9901 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9961 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
    10021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
    10081 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10141 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10201 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
    10261 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10321 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
    10381 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
    10441 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
    10501 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
    10561 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
    10621 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
    10681 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
    10741 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
    10801 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
    10861 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
    10921 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
    10981 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
    11041 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
    11101 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    11161 ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc
    11221 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    11281 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    11341 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    11401 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    11461 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    11521 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    11581 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    11641 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    11701 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    11761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    12061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
    12121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12361 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12961 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    13021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    13081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    13141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    13201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    13261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    13321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    13381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    13441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13561 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13621 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    13681 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
    13741 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    13801 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    13861 ttagg
//
