LOCUS       V039464                11638 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039464
VERSION     V039464
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..6294
                     /label="BRCA1(NM_001407593)"
                     /note="BRCA1(NM_001407593)"
                     /gene="BRCA1"
     CDS             6301..6390
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    6414..7002
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7068..7116
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7318..7746
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7760..8089
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8156..8950
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9126..9247
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9296..9312)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9320..9336
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9344..9374)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9389..9410
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9698..10286)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10457..11317)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11318..11422)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga tttatctgct
      721 cttcgcgttg aagaagtaca aaatgtcatt aatgctatgc agaaaatctt agagtgtccc
      781 atctgtctgg agttgatcaa ggaacctgtc tccacaaagt gtgaccacat attttgcaaa
      841 ttttgcatgc tgaaacttct caaccagaag aaagggcctt cacagtgtcc tttatgtaag
      901 aatgatataa ccaaaaggag cctacaagaa agtacgagat ttagtcaact tgttgaagag
      961 ctattgaaaa tcatttgtgc ttttcagctt gacacaggtt tggagtatgc aaacagctat
     1021 aattttgcaa aaaaggaaaa taactctcct gaacatctaa aagatgaagt ttctatcatc
     1081 caaagtatgg gctacagaaa ccgtgccaaa agacttctac agagtgaacc cgaaaatcct
     1141 tccttgcagg aaaccagtct cagtgtccaa ctctctaacc ttggaactgt gagaactctg
     1201 aggacaaagc agcggataca acctcaaaag acgtctgtct acattgaatt gggatctgat
     1261 tcttctgaag ataccgttaa taaggcaact tattgcagtg tgggagatca agaattgtta
     1321 caaatcaccc ctcaaggaac cagggatgaa atcagtttgg attctgcaaa aaaggctgct
     1381 tgtgaatttt ctgagacgga tgtaacaaat actgaacatc atcaacccag taataatgat
     1441 ttgaacacca ctgagaagcg tgcagctgag aggcatccag aaaagtatca gggtagttct
     1501 gtttcaaact tgcatgtgga gccatgtggc acaaatactc atgccagctc attacagcat
     1561 gagaacagca gtttattact cactaaagac agaatgaatg tagaaaaggc tgaattctgt
     1621 aataaaagca aacagcctgg cttagcaagg agccaacata acagatgggc tggaagtaag
     1681 gaaacatgta atgataggcg gactcccagc acagaaaaaa aggtagatct gaatgctgat
     1741 cccctgtgtg agagaaaaga atggaataag cagaaactgc catgctcaga gaatcctaga
     1801 gatactgaag atgttccttg gataacacta aatagcagca ttcagaaagt taatgagtgg
     1861 ttttccagaa gtgatgaact gttaggttct gatgactcac atgatgggga gtctgaatca
     1921 aatgccaaag tagctgatgt attggacgtt ctaaatgagg tagatgaata ttctggttct
     1981 tcagagaaaa tagacttact ggccagtgat cctcatgagg ctttaatatg taaaagtgaa
     2041 agagttcact ccaaatcagt agagagtaat attgaagaca aaatatttgg gaaaacctat
     2101 cggaagaagg caagcctccc caacttaagc catgtaactg aaaatctaat tataggagca
     2161 tttgttactg agccacagat aatacaagag cgtcccctca caaataaatt aaagcgtaaa
     2221 aggagaccta catcaggcct tcatcctgag gattttatca agaaagcaga tttggcagtt
     2281 caaaagactc ctgaaatgat aaatcaggga actaaccaaa cggagcagaa tggtcaagtg
     2341 atgaatatta ctaatagtgg tcatgagaat aaaacaaaag gtgattctat tcagaatgag
     2401 aaaaatccta acccaataga atcactcgaa aaagaatctg ctttcaaaac gaaagctgaa
     2461 cctataagca gcagtataag caatatggaa ctcgaattaa atatccacaa ttcaaaagca
     2521 cctaaaaaga ataggctgag gaggaagtct tctaccaggc atattcatgc gcttgaacta
     2581 gtagtcagta gaaatctaag cccacctaat tgtactgaat tgcaaattga tagttgttct
     2641 agcagtgaag agataaagaa aaaaaagtac aaccaaatgc cagtcaggca cagcagaaac
     2701 ctacaactca tggaaggtaa agaacctgca actggagcca agaagagtaa caagccaaat
     2761 gaacagacaa gtaaaagaca tgacagcgat actttcccag agctgaagtt aacaaatgca
     2821 cctggttctt ttactaagtg ttcaaatacc agtgaactta aagaatttgt caatcctagc
     2881 cttccaagag aagaaaaaga agagaaacta gaaacagtta aagtgtctaa taatgctgaa
     2941 gaccccaaag atctcatgtt aagtggagaa agggttttgc aaactgaaag atctgtagag
     3001 agtagcagta tttcattggt acctggtact gattatggca ctcaggaaag tatctcgtta
     3061 ctggaagtta gcactctagg gaaggcaaaa acagaaccaa ataaatgtgt gagtcagtgt
     3121 gcagcatttg aaaaccccaa gggactaatt catggttgtt ccaaagataa tagaaatgac
     3181 acagaaggct ttaagtatcc attgggacat gaagttaacc acagtcggga aacaagcata
     3241 gaaatggaag aaagtgaact tgatgctcag tatttgcaga atacattcaa ggtttcaaag
     3301 cgccagtcat ttgctccgtt ttcaaatcca ggaaatgcag aagaggaatg tgcaacattc
     3361 tctgcccact ctgggtcctt aaagaaacaa agtccaaaag tcacttttga atgtgaacaa
     3421 aaggaagaaa atcaaggaaa gaatgagtct aatatcaagc ctgtacagac agttaatatc
     3481 actgcaggct ttcctgtggt tggtcagaaa gataagccag ttgataatgc caaatgtagt
     3541 atcaaaggag gctctaggtt ttgtctatca tctcagttca gaggcaacga aactggactc
     3601 attactccaa ataaacatgg acttttacaa aacccatatc gtataccacc actttttccc
     3661 atcaagtcat ttgttaaaac taaatgtaag aaaaatctgc tagaggaaaa ctttgaggaa
     3721 cattcaatgt cacctgaaag agaaatggga aatgagaaca ttccaagtac agtgagcaca
     3781 attagccgta ataacattag agaaaatgtt tttaaagaag ccagctcaag caatattaat
     3841 gaagtaggtt ccagtactaa tgaagtgggc tccagtatta atgaaatagg ttccagtgat
     3901 gaaaacattc aagcagaact aggtagaaac agagggccaa aattgaatgc tatgcttaga
     3961 ttaggggttt tgcaacctga ggtctataaa caaagtcttc ctggaagtaa ttgtaagcat
     4021 cctgaaataa aaaagcaaga atatgaagaa gtagttcaga ctgttaatac agatttctct
     4081 ccatatctga tttcagataa cttagaacag cctatgggaa gtagtcatgc atctcaggtt
     4141 tgttctgaga cacctgatga cctgttagat gatggtgaaa taaaggaaga tactagtttt
     4201 gctgaaaatg acattaagga aagttctgct gtttttagca aaagcgtcca gaaaggagag
     4261 cttagcagga gtcctagccc tttcacccat acacatttgg ctcagggtta ccgaagaggg
     4321 gccaagaaat tagagtcctc agaagagaac ttatctagtg aggatgaaga gcttccctgc
     4381 ttccaacact tgttatttgg taaagtaaac aatatacctt ctcagtctac taggcatagc
     4441 accgttgcta ccgagtgtct gtctaagaac acagaggaga atttattatc attgaagaat
     4501 agcttaaatg actgcagtaa ccaggtaata ttggcaaagg catctcagga acatcacctt
     4561 agtgaggaaa caaaatgttc tgctagcttg ttttcttcac agtgcagtga attggaagac
     4621 ttgactgcaa atacaaacac ccaggatcct ttcttgattg gttcttccaa acaaatgagg
     4681 catcagtctg aaagccaggg agttggtctg agtgacaagg aattggtttc agatgatgaa
     4741 gaaagaggaa cgggcttgga agaaaataat caagaagagc aaagcatgga ttcaaactta
     4801 ggtgaagcag catctgggtg tgagagtgaa acaagcgtct ctgaagactg ctcagggcta
     4861 tcctctcaga gtgacatttt aaccactcag cagagggata ccatgcaaca taacctgata
     4921 aagctccagc aggaaatggc tgaactagaa gctgtgttag aacagcatgg gagccagcct
     4981 tctaacagct acccttccat cataagtgac tcttctgccc ttgaggacct gcgaaatcca
     5041 gaacaaagca catcagaaaa agcagtatta acttcacaga aaagtagtga ataccctata
     5101 agccagaatc cagaaggcct ttctgctgac aagtttgagg tgtctgcaga tagttctacc
     5161 agtaaaaata aagaaccagg agtggaaagg tcatcccctt ctaaatgccc atcattagat
     5221 gataggtggt acatgcacag ttgctctggg agtcttcaga atagaaacta cccatctcaa
     5281 gaggagctca ttaaggttgt tgatgtggag gagcaacagc tggaagagtc tgggccacac
     5341 gatttgacgg aaacatctta cttgccaagg caagatctag agggaacccc ttacctggaa
     5401 tctggaatca gcctcttctc tgatgaccct gaatctgatc cttctgaaga cagagcccca
     5461 gagtcagctc gtgttggcaa cataccatct tcaacctctg cattgaaagt tccccaattg
     5521 aaagttgcag aatctgccca gagtccagct gctgctcata ctactgatac tgctgggtat
     5581 aatgcaatgg aagaaagtgt gagcagggag aagccagaat tgacagcttc aacagaaagg
     5641 gtcaacaaaa gaatgtccat ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg
     5701 tacaagtttg ccagaaaaca ccacatcact ttaactaatc taattactga agagactact
     5761 catgttgtta tgaaaacaga tgctgagttt gtgtgtgaac ggacactgaa atattttcta
     5821 ggaattgcgg gaggaaaatg ggtagttagc tatttctggg tgacccagtc tattaaagaa
     5881 agaaaaatgc tgaatgagca tgattttgaa gtcagaggag atgtggtcaa tggaagaaac
     5941 caccaaggtc caaagcgagc aagagaatcc caggacagaa agatcttcag ggggctagaa
     6001 atctgttgct atgggccctt caccaacatg cccacagatc aactggaatg gatggtacag
     6061 ctgtgtggtg cttctgtggt gaaggagctt tcatcattca cccttggcac aggtgtccac
     6121 ccaattgtgg ttgtgcagcc agatgcctgg acagaggaca atggcttcca tgcaattggg
     6181 cagatgtgtg aggcacctgt ggtgacccga gagtgggtgt tggacagtgt agcactctac
     6241 cagtgccagg agctggacac ctacctgata ccccagatcc cccacagcca ctacctcgag
     6301 gaacaaaaac taatatcaga ggaagatttg gaacagaaac taatcagtga agaagattta
     6361 gagcaaaagt taatttccga ggaggactta tagtgagggc ccgatatctc gacaatcaac
     6421 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6481 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6541 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6601 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6661 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6721 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6781 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6841 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6901 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6961 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct
     7021 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     7081 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     7141 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     7201 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     7261 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg
     7321 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7381 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7441 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     7501 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7561 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7621 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7681 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7741 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7801 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7861 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7921 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7981 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8041 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8101 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     8161 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     8221 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     8281 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     8341 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     8401 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     8461 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     8521 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     8581 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     8641 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     8701 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     8761 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     8821 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     8881 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     8941 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc
     9001 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     9061 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     9121 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9241 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     9301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     9901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    11461 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata
    11521 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
    11581 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg
//
