LOCUS       V026336                 9282 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026336
VERSION     V026336
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             648..1301
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             1350..3992
                     /label="BRAT1(NM_001350626)"
                     /note="BRAT1(NM_001350626)"
                     /gene="BRAT1"
     misc_feature    4058..4646
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4712..4760
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4962..5390
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5404..5733
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5800..6594
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6770..6891
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6940..6956)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6964..6980
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6988..7018)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7033..7054
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7342..7930)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8101..8961)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8962..9066)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcccaccatg tcccctatac
      661 taggttattg gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg
      721 aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa
      781 agtttgaatt gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat
      841 taacacagtc tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt
      901 gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg
      961 gtgtttcgag aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca
     1021 agctacctga aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg
     1081 gtgatcatgt aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca
     1141 tggacccaat gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag
     1201 ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg
     1261 gctggcaagc cacgtttggt ggtggcgacc atcctccaaa aggaggaggg ggaagcggcg
     1321 gcggagggag taagcttggt accgccacca tggacccaga atgcgcccag ctgctcccgg
     1381 ctctctgtgc tgttctggta gatcccaggc agccggtggc agatgacacc tgtttggaga
     1441 agctcctgga ctggtttaaa acggtcactg aaggagagtc cagtgtcgtg ctgctgcagg
     1501 agcacccctg cctggtggag ctgctgtccc atgtgctgaa agtccaggac ctgagttctg
     1561 gggtcctctc cttctcactg cgcctggcag gaaccttcgc agcccaggaa aactgcttcc
     1621 agtatcttca gcagggggag ttactaccag ggctctttgg ggagccagga cccctcggcc
     1681 gagcaacctg ggccgtcccc accgtgcgca gcggctggat ccagggcctg cgctccctgg
     1741 cacagcaccc cagcgccctg cgcttcctgg ccgaccatgg tgcggtcgac accatcttct
     1801 ccctgcaggg agactccagc ctgtttgtgg cctcggcggc cagtcagctc ctggtgcacg
     1861 tcctggcttt gtccatgcga ggtggagccg aggggcagcc ctgcctgccg gggggtgact
     1921 ggcccgcgtg tgcccagaag atcatggatc acgttgaaga gtccttgtgc tccgcggcca
     1981 cccccaaggt cactcaggcc ctgaacgtcc tgaccacgac cttcgggcgc tgccagagcc
     2041 cctggacgga agccctgtgg gtgcggctga gtccccgcgt ggcctgtctg ctggagagag
     2101 accccatccc cgccgcacac tcgttcgtgg acctgcttct ctgtgtggct cgttctcccg
     2161 tgttcagttc ttccgacggc agcctgtggg agacagtggc gcgggctctg agctgcctgg
     2221 gtcccaccca catgggaccc ctggctttgg ggatcctgaa gctcgagcac tgtccacagg
     2281 cactgaggac ccaggccttc caggtccttc tccagcccct ggcctgtgtc ctgaaggcca
     2341 cggttcaggc ccccggaccc ccaggcttgc tggacgggac ggcagacgat gccacgacgg
     2401 tggacacact cctggcctcc aagtcgtcct gcgccggcct cctgtgccgc accctggctc
     2461 acctggagga gctgcagccg ctgccccagc gcccttcacc gtggccccag gcgtctctac
     2521 tgggggctac agtgactgtc ctgcggctct gtgacggctc ggctgcccct gcctccagtg
     2581 tggggggcca cctctgtggg accctggcgg gctgcgtccg ggtccagcga gcagccctcg
     2641 acttcctggg gacgctgtca caggggacag gcccccagga gctggtgacg caggcgcttg
     2701 ctgtcctcct ggagtgcctc gagagccccg gctccagccc cacggttctg aagaaggcct
     2761 tccaggccac gctcaggtgg ctcctgagct cacccaagac ccccggctgc tctgatctcg
     2821 gccccctcat cccgcagttc ctcagagagc tgttccctgt gctgcagaaa cgcctgtgcc
     2881 acccctgctg ggaggtgagg gactccgccc tcgagttcct gacccagctg agcaggcact
     2941 ggggaggaca ggctgacttc agatgcgcac tcttggcttc agaggtgcct cagctggccc
     3001 tgcagctcct ccaggaccct gagagttatg tccgagcgag tgcagtgacc gccatggggc
     3061 agctgtccag ccagggcctg cacgccccca ccagccctga gcatgcagag gcccggcaga
     3121 tgggtaaact gaggattgga gggccgtgtg ctcactgcgc agcatgggaa ggtgtcagag
     3181 cgggctgtgg ccctagactg catgtccggg gccagccccc atcatgtacc ggagtcctgt
     3241 tgcgggagcc tcgctcctgc caccccacca accatccgca cttgctccct gtcccccaga
     3301 gcctgttcct ggagctcctg cacatcctct ccgtagactc ggagggcttc ccacggcggg
     3361 cggtcatgca agtcttcact gagtggctgc gggacggcca cgccgacgcg gcccaggaca
     3421 cggagcagtt cgtggccact gtgctgcagg cggcgagccg agacctggac tgggaggtcc
     3481 gcgcccaggg cctggagctg gccctcgtgt tcctgggcca gactttgggg ccgccgcgta
     3541 cccactgccc ctatgccgtg gccctacccg aggtggcccc agcccagcca ctcaccgagg
     3601 cactgagggc tctctgccac gtggggctct ttgacttcgc cttttgtgcc ttgtttgact
     3661 gcgaccgccc tgtggcgcag aagtcttgtg acctccttct cttcctgagg gacaagattg
     3721 cttcctacag cagcctgcgg gaggccaggg gcagccccaa cactgcctcc gcagaggcca
     3781 ccctgccgag gtggcgggcg ggtgagcagg cccagccccc aggggaccag gagcctgagg
     3841 ctgtgctggc catgctcagg tccctagacc tggagggcct gcggagcacg ctggccgaga
     3901 gcagcgacca cgtggaaaag agtccccagt ccctcctgca ggacatgctg gccacgggag
     3961 gcttcctgca gggggacgag gccgactgct actgagaatt ctgcagatat ccagcacagt
     4021 ggcggccgct cgagtctaga gggcccgata tctcgacaat caacctctgg attacaaaat
     4081 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4141 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4201 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4261 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4321 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4381 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4441 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4501 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4561 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4621 gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga aacacggaag
     4681 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4741 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     4801 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     4861 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     4921 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4981 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5041 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5101 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga
     5161 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5221 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5281 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc
     5341 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5401 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5461 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5521 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5581 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5641 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5701 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5761 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5821 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5881 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5941 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6001 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6061 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6121 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6181 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6241 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6301 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     6361 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6421 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6481 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6541 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     6601 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     6661 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6721 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6901 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6961 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7021 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7081 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7141 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7501 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     7561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7741 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7981 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8041 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8101 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8161 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8221 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8281 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8341 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8401 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8461 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8521 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8581 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8641 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8701 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8761 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8821 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8881 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8941 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9001 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9061 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     9121 cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     9181 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     9241 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
