LOCUS V019132 8120 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019132 VERSION V019132 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..2800 /label="BCL6(NM_001706)" /note="BCL6(NM_001706)" /gene="BCL6" CDS 2807..2872 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 2896..3484 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3550..3598 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3800..4228 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4242..4571 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4638..5432 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5608..5729 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5778..5794) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5802..5818 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5826..5856) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5871..5892 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6180..6768) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6939..7799) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7800..7904) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatggcctc gccggctgac agctgtatcc agttcacccg 721 ccatgccagt gatgttcttc tcaaccttaa tcgtctccgg agtcgagaca tcttgactga 781 tgttgtcatt gttgtgagcc gtgagcagtt tagagcccat aaaacggtcc tcatggcctg 841 cagtggcctg ttctatagca tctttacaga ccagttgaaa tgcaacctta gtgtgatcaa 901 tctagatcct gagatcaacc ctgagggatt ctgcatcctc ctggacttca tgtacacatc 961 tcggctcaat ttgcgggagg gcaacatcat ggctgtgatg gccacggcta tgtacctgca 1021 gatggagcat gttgtggaca cttgccggaa gtttattaag gccagtgaag cagagatggt 1081 ttctgccatc aagcctcctc gtgaagagtt cctcaacagc cggatgctga tgccccaaga 1141 catcatggcc tatcggggtc gtgaggtggt ggagaacaac ctgccactga ggagcgcccc 1201 tgggtgtgag agcagagcct ttgcccccag cctgtacagt ggcctgtcca caccgccagc 1261 ctcttattcc atgtacagcc acctccctgt cagcagcctc ctcttctccg atgaggagtt 1321 tcgggatgtc cggatgcctg tggccaaccc cttccccaag gagcgggcac tcccatgtga 1381 tagtgccagg ccagtccctg gtgagtacag ccggccgact ttggaggtgt cccccaatgt 1441 gtgccacagc aatatctatt cacccaagga aacaatccca gaagaggcac gaagtgatat 1501 gcactacagt gtggctgagg gcctcaaacc tgctgccccc tcagcccgaa atgcccccta 1561 cttcccttgt gacaaggcca gcaaagaaga agagagaccc tcctcggaag atgagattgc 1621 cctgcatttc gagcccccca atgcacccct gaaccggaag ggtctggtta gtccacagag 1681 cccccagaaa tctgactgcc agcccaactc gcccacagag tcctgcagca gtaagaatgc 1741 ctgcatcctc caggcttctg gctcccctcc agccaagagc cccactgacc ccaaagcctg 1801 caactggaag aaatacaagt tcatcgtgct caacagcctc aaccagaatg ccaaaccaga 1861 ggggcctgag caggctgagc tgggccgcct ttccccacga gcctacacgg ccccacctgc 1921 ctgccagcca cccatggagc ctgagaacct tgacctccag tccccaacca agctgagtgc 1981 cagcggggag gactccacca tcccacaagc cagccggctc aataacatcg ttaacaggtc 2041 catgacgggc tctccccgca gcagcagcga gagccactca ccactctaca tgcacccccc 2101 gaagtgcacg tcctgcggct ctcagtcccc acagcatgca gagatgtgcc tccacaccgc 2161 tggccccacg ttccctgagg agatgggaga gacccagtct gagtactcag attctagctg 2221 tgagaacggg gccttcttct gcaatgagtg tgactgccgc ttctctgagg aggcctcact 2281 caagaggcac acgctgcaga cccacagtga caaaccctac aagtgtgacc gctgccaggc 2341 ctccttccgc tacaagggca acctcgccag ccacaagacc gtccataccg gtgagaaacc 2401 ctatcgttgc aacatctgtg gggcccagtt caaccggcca gccaacctga aaacccacac 2461 tcgaattcac tctggagaga agccctacaa atgcgaaacc tgcggagcca gatttgtaca 2521 ggtggcccac ctccgtgccc atgtgcttat ccacactggt gagaagccct atccctgtga 2581 aatctgtggc acccgtttcc ggcaccttca gactctgaag agccacctgc gaatccacac 2641 aggagagaaa ccttaccatt gtgagaagtg taacctgcat ttccgtcaca aaagccagct 2701 gcgacttcac ttgcgccaga agcatggcgc catcaccaac accaaggtgc aataccgcgt 2761 gtcagccact gacctgcctc cggagctccc caaagcctgc ctcgaggact acaaagacca 2821 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagtgagg 2881 gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 2941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 3001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 3061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 3121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 3181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 3241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 3301 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 3361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 3421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 3481 ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 3541 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat 3601 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 3661 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 3721 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg 3781 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 3841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 3901 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc 3961 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 4021 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 4081 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 4141 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag 4201 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 4261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4321 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4381 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 4441 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 4501 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4561 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag 4621 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 4681 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 4741 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 4801 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 4861 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 4921 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 4981 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 5041 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 5101 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 5161 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 5221 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 5281 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 5341 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 5401 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc 5461 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 5521 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 5581 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 5641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 5701 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 5761 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 5821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 5881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 5941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 6001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6361 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6601 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 6661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 7021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 7081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 7141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 7201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 7261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 7321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 7381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 7441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 7501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 7561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 7621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 7681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 7741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 7801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 7861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 7921 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta 7981 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 8041 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 8101 gcatgaagaa tctgcttagg //