LOCUS       V019132                 8120 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019132
VERSION     V019132
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..2800
                     /label="BCL6(NM_001706)"
                     /note="BCL6(NM_001706)"
                     /gene="BCL6"
     CDS             2807..2872
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    2896..3484
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3550..3598
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3800..4228
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4242..4571
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4638..5432
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5608..5729
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5778..5794)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5802..5818
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5826..5856)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5871..5892
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6180..6768)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6939..7799)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7800..7904)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatggcctc gccggctgac agctgtatcc agttcacccg
      721 ccatgccagt gatgttcttc tcaaccttaa tcgtctccgg agtcgagaca tcttgactga
      781 tgttgtcatt gttgtgagcc gtgagcagtt tagagcccat aaaacggtcc tcatggcctg
      841 cagtggcctg ttctatagca tctttacaga ccagttgaaa tgcaacctta gtgtgatcaa
      901 tctagatcct gagatcaacc ctgagggatt ctgcatcctc ctggacttca tgtacacatc
      961 tcggctcaat ttgcgggagg gcaacatcat ggctgtgatg gccacggcta tgtacctgca
     1021 gatggagcat gttgtggaca cttgccggaa gtttattaag gccagtgaag cagagatggt
     1081 ttctgccatc aagcctcctc gtgaagagtt cctcaacagc cggatgctga tgccccaaga
     1141 catcatggcc tatcggggtc gtgaggtggt ggagaacaac ctgccactga ggagcgcccc
     1201 tgggtgtgag agcagagcct ttgcccccag cctgtacagt ggcctgtcca caccgccagc
     1261 ctcttattcc atgtacagcc acctccctgt cagcagcctc ctcttctccg atgaggagtt
     1321 tcgggatgtc cggatgcctg tggccaaccc cttccccaag gagcgggcac tcccatgtga
     1381 tagtgccagg ccagtccctg gtgagtacag ccggccgact ttggaggtgt cccccaatgt
     1441 gtgccacagc aatatctatt cacccaagga aacaatccca gaagaggcac gaagtgatat
     1501 gcactacagt gtggctgagg gcctcaaacc tgctgccccc tcagcccgaa atgcccccta
     1561 cttcccttgt gacaaggcca gcaaagaaga agagagaccc tcctcggaag atgagattgc
     1621 cctgcatttc gagcccccca atgcacccct gaaccggaag ggtctggtta gtccacagag
     1681 cccccagaaa tctgactgcc agcccaactc gcccacagag tcctgcagca gtaagaatgc
     1741 ctgcatcctc caggcttctg gctcccctcc agccaagagc cccactgacc ccaaagcctg
     1801 caactggaag aaatacaagt tcatcgtgct caacagcctc aaccagaatg ccaaaccaga
     1861 ggggcctgag caggctgagc tgggccgcct ttccccacga gcctacacgg ccccacctgc
     1921 ctgccagcca cccatggagc ctgagaacct tgacctccag tccccaacca agctgagtgc
     1981 cagcggggag gactccacca tcccacaagc cagccggctc aataacatcg ttaacaggtc
     2041 catgacgggc tctccccgca gcagcagcga gagccactca ccactctaca tgcacccccc
     2101 gaagtgcacg tcctgcggct ctcagtcccc acagcatgca gagatgtgcc tccacaccgc
     2161 tggccccacg ttccctgagg agatgggaga gacccagtct gagtactcag attctagctg
     2221 tgagaacggg gccttcttct gcaatgagtg tgactgccgc ttctctgagg aggcctcact
     2281 caagaggcac acgctgcaga cccacagtga caaaccctac aagtgtgacc gctgccaggc
     2341 ctccttccgc tacaagggca acctcgccag ccacaagacc gtccataccg gtgagaaacc
     2401 ctatcgttgc aacatctgtg gggcccagtt caaccggcca gccaacctga aaacccacac
     2461 tcgaattcac tctggagaga agccctacaa atgcgaaacc tgcggagcca gatttgtaca
     2521 ggtggcccac ctccgtgccc atgtgcttat ccacactggt gagaagccct atccctgtga
     2581 aatctgtggc acccgtttcc ggcaccttca gactctgaag agccacctgc gaatccacac
     2641 aggagagaaa ccttaccatt gtgagaagtg taacctgcat ttccgtcaca aaagccagct
     2701 gcgacttcac ttgcgccaga agcatggcgc catcaccaac accaaggtgc aataccgcgt
     2761 gtcagccact gacctgcctc cggagctccc caaagcctgc ctcgaggact acaaagacca
     2821 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagtgagg
     2881 gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     3301 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3481 ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     3541 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     3601 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     3661 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     3721 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg
     3781 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3901 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     3961 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4021 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4081 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4141 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     4201 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     4261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4321 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4381 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4441 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4501 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4561 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     4621 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4681 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4741 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4801 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4861 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4921 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4981 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5041 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5101 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5161 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5221 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5281 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5341 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5401 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc
     5461 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5521 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5581 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5701 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5761 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6361 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6601 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7921 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta
     7981 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     8041 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     8101 gcatgaagaa tctgcttagg
//
