LOCUS V031197 6471 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031197 VERSION V031197 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2618 /label="Asic3(NM_183000)" /note="Asic3(NM_183000)" /gene="Asic3" CDS 2637..2666 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2685..2708 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2728..2744) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2755..3377 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3526..4114) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4443..4490 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4722..5516) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5551..5908) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5910..6014) /gene="bla" /label="AmpR promoter" rep_origin 6041..6469 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaaacctccc tcaggactgg aggaggccca gcggcgacag gcctcagaca 1081 tccgggtgtt cgccaacagc tgcacgatgc atggtttggg ccacatcttt ggccctggag 1141 gcctgaccct gcgccgtggg ctgtgggcca cagctgtact cctgtcgctg gcggccttcc 1201 tctaccaggt ggctgagcgg gttcgctact atggggagtt ccaccataag accaccctgg 1261 atgagcgtga gagccaccag cttaccttcc cagctgtcac tttgtgtaac atcaatcccc 1321 tgcgccgctc acgcctcaca cccaatgact tgcactgggc cggaacggca ctgctgggtc 1381 tggaccctgc tgaacatgct gcctaccttc gtgccctggg ccagccccct gcaccacctg 1441 gcttcatgcc cagtccgact tttgacatgg cacaactcta cgccagagcc ggccactccc 1501 ttgaggacat gttgctggac tgccgatacc gtggccagcc ctgtggacct gagaacttca 1561 cagtgatttt cactcgaatg gggcaatgct acaccttcaa ctctggtgcc cagggggcag 1621 agctgctcac cactcctaag ggcggtgctg gcaatggact ggagattatg ctggatgtac 1681 agcaggagga gtatctgccc atctggaagg acatggaaga gaccccattt gaggtgggga 1741 tccgagtgca gatccacggc caggaggaac cccctgccat tgaccagctg ggcttcggtg 1801 ctgccccagg ccaccagact tttgtgtcct gccagcaaca gcaactgagt ttcctgccac 1861 caccctgggg tgactgcaat accgcatctg tggatcccga ctttgatcca gagccctctg 1921 atcccctggg ttcccctagc tccagccctc cttatagctt aatagggtgt cgcctggcct 1981 gtgagtcacg ctatgtggct cggaagtgcg gatgtcgaat gatgcatatg cctggaaact 2041 ccccagtgtg cagcccccag cagtacaagg actgtgccag cccagctctg gacgctatgc 2101 tgcgaaagga cacttgtgtc tgtcccaacc cgtgcgccac tacacgctat gccaaggagc 2161 tctccatggt gcggattccc agccgcgctt cagctcgcta cctggcccgg aaatacaacc 2221 gtagcgagac ttacatcacg gagaatgtac tggttctgga tatcttcttt gaagccctca 2281 actatgaggc cgtggaacaa aaggcagctt atgaagtgtc ggagttgctg ggagacattg 2341 ggggacagat gggactgttt atcggagcca gcctgcttac catcctcgag atcctcgact 2401 acctctgtga ggtttttcaa gacagagtcc tggggtactt ctggaacaga aggagctctc 2461 aaaggcgctc tggcaacact ctgctccagg aagagttgaa tgggcatcga acacatgttc 2521 cccatctcag cctaggcccc aggcctccta ccgctcccag tgctgtcacc aagacactcg 2581 ctgcctccca ccgtacctgt tacctcgtga caaggctcac gcgtacgcgg ccgctcgagc 2641 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg 2701 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg 2761 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc 2821 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat 2881 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt 2941 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact 3001 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga 3061 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc 3121 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct 3181 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta 3241 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa 3301 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag 3361 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc 3421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3721 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 3961 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4261 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg 4321 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc 4381 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt 4441 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg 4501 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa 4561 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat 4621 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt 4681 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 4741 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 4801 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 4861 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 4921 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg 4981 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 5041 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 5101 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 5161 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 5221 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 5281 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 5341 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 5401 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 5461 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga 5521 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc 5581 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa 5641 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat 5701 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact 5761 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat 5821 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac 5881 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt 5941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 6001 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 6061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 6121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 6181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 6241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 6301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 6361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 6421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t //