LOCUS       V031197                 6471 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031197
VERSION     V031197
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2618
                     /label="Asic3(NM_183000)"
                     /note="Asic3(NM_183000)"
                     /gene="Asic3"
     CDS             2637..2666
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2685..2708
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2728..2744)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2755..3377
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3526..4114)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4443..4490
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4722..5516)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5551..5908)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5910..6014)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6041..6469
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaaacctccc tcaggactgg aggaggccca gcggcgacag gcctcagaca
     1081 tccgggtgtt cgccaacagc tgcacgatgc atggtttggg ccacatcttt ggccctggag
     1141 gcctgaccct gcgccgtggg ctgtgggcca cagctgtact cctgtcgctg gcggccttcc
     1201 tctaccaggt ggctgagcgg gttcgctact atggggagtt ccaccataag accaccctgg
     1261 atgagcgtga gagccaccag cttaccttcc cagctgtcac tttgtgtaac atcaatcccc
     1321 tgcgccgctc acgcctcaca cccaatgact tgcactgggc cggaacggca ctgctgggtc
     1381 tggaccctgc tgaacatgct gcctaccttc gtgccctggg ccagccccct gcaccacctg
     1441 gcttcatgcc cagtccgact tttgacatgg cacaactcta cgccagagcc ggccactccc
     1501 ttgaggacat gttgctggac tgccgatacc gtggccagcc ctgtggacct gagaacttca
     1561 cagtgatttt cactcgaatg gggcaatgct acaccttcaa ctctggtgcc cagggggcag
     1621 agctgctcac cactcctaag ggcggtgctg gcaatggact ggagattatg ctggatgtac
     1681 agcaggagga gtatctgccc atctggaagg acatggaaga gaccccattt gaggtgggga
     1741 tccgagtgca gatccacggc caggaggaac cccctgccat tgaccagctg ggcttcggtg
     1801 ctgccccagg ccaccagact tttgtgtcct gccagcaaca gcaactgagt ttcctgccac
     1861 caccctgggg tgactgcaat accgcatctg tggatcccga ctttgatcca gagccctctg
     1921 atcccctggg ttcccctagc tccagccctc cttatagctt aatagggtgt cgcctggcct
     1981 gtgagtcacg ctatgtggct cggaagtgcg gatgtcgaat gatgcatatg cctggaaact
     2041 ccccagtgtg cagcccccag cagtacaagg actgtgccag cccagctctg gacgctatgc
     2101 tgcgaaagga cacttgtgtc tgtcccaacc cgtgcgccac tacacgctat gccaaggagc
     2161 tctccatggt gcggattccc agccgcgctt cagctcgcta cctggcccgg aaatacaacc
     2221 gtagcgagac ttacatcacg gagaatgtac tggttctgga tatcttcttt gaagccctca
     2281 actatgaggc cgtggaacaa aaggcagctt atgaagtgtc ggagttgctg ggagacattg
     2341 ggggacagat gggactgttt atcggagcca gcctgcttac catcctcgag atcctcgact
     2401 acctctgtga ggtttttcaa gacagagtcc tggggtactt ctggaacaga aggagctctc
     2461 aaaggcgctc tggcaacact ctgctccagg aagagttgaa tgggcatcga acacatgttc
     2521 cccatctcag cctaggcccc aggcctccta ccgctcccag tgctgtcacc aagacactcg
     2581 ctgcctccca ccgtacctgt tacctcgtga caaggctcac gcgtacgcgg ccgctcgagc
     2641 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg
     2701 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg
     2761 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     2821 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     2881 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     2941 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     3001 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     3061 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     3121 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     3181 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta
     3241 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa
     3301 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag
     3361 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc
     3421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3721 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3961 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4261 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     4321 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     4381 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     4441 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     4501 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa
     4561 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     4621 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     4681 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     4741 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     4801 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     4861 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     4921 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     4981 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     5041 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     5101 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     5161 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     5221 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     5281 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
     5341 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     5401 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     5461 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     5521 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc
     5581 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     5641 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     5701 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     5761 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     5821 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     5881 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt
     5941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6001 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     6061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t
//
