LOCUS V027975 7246 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027975 VERSION V027975 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1905 /label="Adrb3(NM_013462)" /note="Adrb3(NM_013462)" /gene="Adrb3" CDS 1912..1938 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1942..1968 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1972..1998 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2022..2610 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2676..2724 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2926..3354 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3368..3697 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3764..4558 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4734..4855 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4904..4920) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4928..4944 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4952..4982) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4997..5018 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5306..5894) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6065..6925) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6926..7030) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc tccgtggcct 721 cacagaaacg gctctctggc tttgtggtcg gacgccccta ccctggaccc cagtgcagcc 781 aacaccagtg ggttgccagg agtaccatgg gcagcggcat tggctggggc attgctggcg 841 ctggccacgg tgggaggcaa cctgctggta atcatagcca tcgcccgcac gccgagacta 901 cagaccataa ccaacgtgtt cgtgacttca ctggccgcag ctgacttggt agtgggactc 961 ctcgtaatgc caccaggggc cacattggcg ctgactggcc attggccctt gggcgaaact 1021 ggttgcgaac tgtggacgtc agtggacgtg ctctgtgtaa ctgctagcat cgagaccttg 1081 tgcgccctgg ctgtggaccg ctacctagct gtcaccaacc ctttgcgtta cggcacgctg 1141 gttaccaagc gccgcgcccg cgcggcagtt gtcctggtgt ggatcgtgtc cgctgccgtg 1201 tcctttgcgc ccatcatgag ccagtggtgg cgtgtagggg cagatgccga ggcacaggaa 1261 tgccactcca atccgcgctg ctgttccttt gcctccaaca tgccctatgc gctgctctcc 1321 tcctccgtct ccttctacct tcccctcctt gtgatgctct tcgtctatgc tcgagtgttc 1381 gttgtggcta agcgccaacg gcatttgctg cgccgggaac tgggccgctt ctcgcccgag 1441 gagtctccgc cgtctccgtc gcgctctccg tcccctgcca caggcgggac acccgcggca 1501 ccggatggag tgcccccctg cggccggcgg cctgcgcgcc tcctgccact ccgggaacac 1561 cgcgccctgc gcaccttagg tctcattatg ggcatcttct ctctgtgctg gctgcccttc 1621 ttcctggcca acgtgctgcg cgcactcgcg gggccctctc tagttcccag cggagttttc 1681 atcgccctga actggctggg ctatgccaac tccgccttca acccggtcat ctactgccgc 1741 agcccggact ttcgcgacgc cttccgtcgt cttctgtgta gctacggtgg ccgtggaccg 1801 gaggagccac gcgcagtcac cttcccagcc agccctgttg aagccaggca gagtccaccg 1861 ctcaacaggt ttgatggcta tgaaggtgcg cgtccgtttc ccacgctcga gtacccatac 1921 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat 1981 gatgtcccag actacgcata gtgagggccc gatatctcga caatcaacct ctggattaca 2041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 2101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 2161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 2221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 2281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 2341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 2401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 2461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 2521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 2581 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg 2641 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 2701 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 2761 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 2821 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 2881 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg 2941 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 3001 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 3061 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 3121 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 3181 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 3241 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 3301 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 3361 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 3421 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 3481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3541 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3601 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 3661 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 3721 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3781 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3841 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3901 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 3961 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4021 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4081 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4141 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4201 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4261 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4321 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4381 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4441 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4501 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4561 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 4621 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 4681 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 4741 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 4801 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 4861 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 4921 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 4981 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 5041 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 5101 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 5161 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 5401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 5461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 5521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 5761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5881 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5941 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6001 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6061 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 6121 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6181 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6241 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6301 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6361 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 6421 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6481 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6541 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6601 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6661 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6721 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6781 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6841 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6901 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 7021 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc 7081 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta 7141 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac 7201 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg //