LOCUS       V044018                15243 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044018
VERSION     V044018
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..9923
                     /label="ATM(NM_001351834)"
                     /note="ATM(NM_001351834)"
                     /gene="ATM"
     CDS             9930..9995
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    10019..10607
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    10673..10721
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      10923..11351
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        11365..11694
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             11761..12555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    12731..12852
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(12901..12917)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12925..12941
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12949..12979)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12994..13015
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(13303..13891)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(14062..14922)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14923..15027)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgag tctagtactt aatgatctgc
      781 ttatctgctg ccgtcaacta gaacatgata gagctacaga acgaaagaaa gaagttgaga
      841 aatttaagcg cctgattcga gatcctgaaa caattaaaca tctagatcgg cattcagatt
      901 ccaaacaagg aaaatatttg aattgggatg ctgtttttag atttttacag aaatatattc
      961 agaaagaaac agaatgtctg agaatagcaa aaccaaatgt atcagcctca acacaagcct
     1021 ccaggcagaa aaagatgcag gaaatcagta gtttggtcaa atacttcatc aaatgtgcaa
     1081 acagaagagc acctaggcta aaatgtcaag aactcttaaa ttatatcatg gatacagtga
     1141 aagattcatc taatggtgct atttacggag ctgattgtag caacatacta ctcaaagaca
     1201 ttctttctgt gagaaaatac tggtgtgaaa tatctcagca acagtggtta gaattgttct
     1261 ctgtgtactt caggctctat ctgaaacctt cacaagatgt tcatagagtt ttagtggcta
     1321 gaataattca tgctgttacc aaaggatgct gttctcagac tgacggatta aattccaaat
     1381 ttttggactt tttttccaag gctattcagt gtgcgagaca agaaaagagc tcttcaggtc
     1441 taaatcatat cttagcagct cttactatct tcctcaagac tttggctgtc aactttcgaa
     1501 ttcgagtgtg tgaattagga gatgaaattc ttcccacttt gctttatatt tggactcaac
     1561 ataggcttaa tgattcttta aaagaagtca ttattgaatt atttcaactg caaatttata
     1621 tccatcatcc gaaaggagcc aaaacccaag aaaaaggtgc ttatgaatca acaaaatgga
     1681 gaagtatttt atacaactta tatgatctgc tagtgaatga gataagtcat ataggaagta
     1741 gaggaaagta ttcttcagga tttcgtaata ttgccgtcaa agaaaatttg attgaattga
     1801 tggcagatat ctgtcaccag gtttttaatg aagataccag atccttggag atttctcaat
     1861 cttacactac tacacaaaga gaatctagtg attacagtgt cccttgcaaa aggaagaaaa
     1921 tagaactagg ctgggaagta ataaaagatc accttcagaa gtcacagaat gattttgatc
     1981 ttgtgccttg gctacagatt gcaacccaat taatatcaaa gtatcctgca agtttaccta
     2041 actgtgagct gtctccatta ctgatgatac tatctcagct tctaccccaa cagcgacatg
     2101 gggaacgtac accatatgtg ttacgatgcc ttacggaagt tgcattgtgt caagacaaga
     2161 ggtcaaacct agaaagctca caaaagtcag atttattaaa actctggaat aaaatttggt
     2221 gtattacctt tcgtggtata agttctgagc aaatacaagc tgaaaacttt ggcttacttg
     2281 gagccataat tcagggtagt ttagttgagg ttgacagaga attctggaag ttatttactg
     2341 ggtcagcctg cagaccttca tgtcctgcag tatgctgttt gactttggca ctgaccacca
     2401 gtatagttcc aggaacggta aaaatgggaa tagagcaaaa tatgtgtgaa gtaaatagaa
     2461 gcttttcttt aaaggaatca ataatgaaat ggctcttatt ctatcagtta gagggtgact
     2521 tagaaaatag cacagaagtg cctccaattc ttcacagtaa ttttcctcat cttgtactgg
     2581 agaaaattct tgtgagtctc actatgaaaa actgtaaagc tgcaatgaat tttttccaaa
     2641 gcgtgccaga gtgtgaacac caccaaaaag ataaagaaga actttcattc tcagaagtag
     2701 aagaactatt tcttcagaca acttttgaca agatggactt tttaaccatt gtgagagaat
     2761 gtggtataga aaagcaccag tccagtattg gcttctctgt ccaccagaat ctcaaggaat
     2821 cactggatcg ctgtcttctg ggattatcag aacagcttct gaataattac tcatctgaga
     2881 ttacaaattc agaaactctt gtccggtgtt cacgtctttt ggtgggtgtc cttggctgct
     2941 actgttacat gggtgtaata gctgaagagg aagcatataa gtcagaatta ttccagaaag
     3001 ccaagtctct aatgcaatgt gcaggagaaa gtatcactct gtttaaaaat aagacaaatg
     3061 aggaattcag aattggttcc ttgagaaata tgatgcagct atgtacacgt tgcttgagca
     3121 actgtaccaa gaagagtcca aataagattg catctggctt tttcctgcga ttgttaacat
     3181 caaagctaat gaatgacatt gcagatattt gtaaaagttt agcatccttc atcaaaaagc
     3241 catttgaccg tggagaagta gaatcaatgg aagatgatac taatggaaat ctaatggagg
     3301 tggaggatca gtcatccatg aatctattta acgattaccc tgatagtagt gttagtgatg
     3361 caaacgaacc tggagagagc caaagtacca taggtgccat taatccttta gctgaagaat
     3421 atctgtcaaa gcaagatcta cttttcttag acatgctcaa gttcttgtgt ttgtgtgtaa
     3481 ctactgctca gaccaatact gtgtccttta gggcagctga tattcggagg aaattgttaa
     3541 tgttaattga ttctagcacg ctagaaccta ccaaatccct ccacctgcat atgtatctaa
     3601 tgcttttaaa ggagcttcct ggagaagagt accccttgcc aatggaagat gttcttgaac
     3661 ttctgaaacc actatccaat gtgtgttctt tgtatcgtcg tgaccaagat gtttgtaaaa
     3721 ctattttaaa ccatgtcctt catgtagtga aaaacctagg tcaaagcaat atggactctg
     3781 agaacacaag ggatgctcaa ggacagtttc taacagtaat tggagcattt tggcatctaa
     3841 caaaggagag gaaatatata ttctctgtaa gaatggccct agtaaattgc cttaaaactt
     3901 tgcttgaggc tgatccttat tcaaaatggg ccattcttaa tgtaatggga aaagactttc
     3961 ctgtaaatga agtatttaca caatttcttg ctgacaatca tcaccaagtt cgcatgttgg
     4021 ctgcagagtc aatcaataga ttgttccagg acacgaaggg agattcttcc aggttactga
     4081 aagcacttcc tttgaagctt cagcaaacag cttttgaaaa tgcatacttg aaagctcagg
     4141 aaggaatgag agaaatgtcc catagtgctg agaaccctga aactttggat gaaatttata
     4201 atagaaaatc tgttttactg acgttgatag ctgtggtttt atcctgtagc cctatctgcg
     4261 aaaaacaggc tttgtttgcc ctgtgtaaat ctgtgaaaga gaatggatta gaacctcacc
     4321 ttgtgaaaaa ggttttagag aaagtttctg aaacttttgg atatagacgt ttagaagact
     4381 ttatggcatc tcatttagat tatctggttt tggaatggct aaatcttcaa gatactgaat
     4441 acaacttatc ttcttttcct tttattttat taaactacac aaatattgag gatttctata
     4501 gatcttgtta taaggttttg attccacatc tggtgattag aagtcatttt gatgaggtga
     4561 agtccattgc taatcagatt caagaggact ggaaaagtct tctaacagac tgctttccaa
     4621 agattcttgt aaatattctt ccttattttg cctatgaggg taccagagac agtgggatgg
     4681 cacagcaaag agagactgct accaaggtct atgatatgct taaaagtgaa aacttattgg
     4741 gaaaacagat tgatcactta ttcattagta atttaccaga gattgtggtg gagttattga
     4801 tgacgttaca tgagccagca aattctagtg ccagtcagag cactgacctc tgtgactttt
     4861 caggggattt ggatcctgct cctaatccac ctcattttcc atcgcatgtg attaaagcaa
     4921 catttgccta tatcagcaat tgtcataaaa ccaagttaaa aagcatttta gaaattcttt
     4981 ccaaaagccc tgattcctat cagaaaattc ttcttgccat atgtgagcaa gcagctgaaa
     5041 caaataatgt ttataagaag cacagaattc ttaaaatata tcacctgttt gttagtttat
     5101 tactgaaaga tataaaaagt ggcttaggag gagcttgggc ctttgttctt cgagacgtta
     5161 tttatacttt gattcactat atcaaccaaa ggccttcttg tatcatggat gtgtcattac
     5221 gtagcttctc cctttgttgt gacttattaa gtcaggtttg ccagacagcc gtgacttact
     5281 gtaaggatgc tctagaaaac catcttcatg ttattgttgg tacacttata ccccttgtgt
     5341 atgagcaggt ggaggttcag aaacaggtat tggacttgtt gaaatactta gtgatagata
     5401 acaaggataa tgaaaacctc tatatcacga ttaagctttt agatcctttt cctgaccatg
     5461 ttgtttttaa ggatttgcgt attactcagc aaaaaatcaa atacagtaga ggaccctttt
     5521 cactcttgga ggaaattaac cattttctct cagtaagtgt ttatgatgca cttccattga
     5581 caagacttga aggactaaag gatcttcgaa gacaactgga actacataaa gatcagatgg
     5641 tggacattat gagagcttct caggataatc cgcaagatgg gattatggtg aaactagttg
     5701 tcaatttgtt gcagttatcc aagatggcaa taaaccacac tggtgaaaaa gaagttctag
     5761 aggctgttgg aagctgcttg ggagaagtgg gtcctataga tttctctacc atagctatac
     5821 aacatagtaa agatgcatct tataccaagg cccttaagtt atttgaagat aaagaacttc
     5881 agtggacctt cataatgctg acctacctga ataacacact ggtagaagat tgtgtcaaag
     5941 ttcgatcagc agctgttacc tgtttgaaaa acattttagc cacaaagact ggacatagtt
     6001 tctgggagat ttataagatg acaacagatc caatgctggc ctatctacag ccttttagaa
     6061 catcaagaaa aaagttttta gaagtaccca gatttgacaa agaaaaccct tttgaaggcc
     6121 tggatgatat aaatctgtgg attcctctaa gtgaaaatca tgacatttgg ataaagacac
     6181 tgacttgtgc ttttttggac agtggaggca caaaatgtga aattcttcaa ttattaaagc
     6241 caatgtgtga agtgaaaact gacttttgtc agactgtact tccatacttg attcatgata
     6301 ttttactcca agatacaaat gaatcatgga gaaatctgct ttctacacat gttcagggat
     6361 ttttcaccag ctgtcttcga cacttctcgc aaacgagccg atccacaacc cctgcaaact
     6421 tggattcaga gtcagagcac tttttccgat gctgtttgga taaaaaatca caaagaacaa
     6481 tgcttgctgt tgtggactac atgagaagac aaaagagacc ttcttcagga acaattttta
     6541 atgatgcttt ctggctggat ttaaattatc tagaagttgc caaggtagct cagtcttgtg
     6601 ctgctcactt tacagcttta ctctatgcag aaatctatgc agataagaaa agtatggatg
     6661 atcaagagaa aagaagtctt gcatttgaag aaggaagcca gagtacaact atttctagct
     6721 tgagtgaaaa aagtaaagaa gaaactggaa taagtttaca ggatcttctc ttagaaatct
     6781 acagaagtat aggggagcca gatagtttgt atggctgtgg tggagggaag atgttacaac
     6841 ccattactag actacgaaca tatgaacacg aagcaatgtg gggcaaagcc ctagtaacat
     6901 atgacctcga aacagcaatc ccctcatcaa cacgccaggc aggaatcatt caggccttgc
     6961 agaatttggg actctgccat attctttccg tctatttaaa aggattggat tatgaaaata
     7021 aagactggtg tcctgaacta gaagaacttc attaccaagc agcatggagg aatatgcagt
     7081 gggaccattg cacttccgtc agcaaagaag tagaaggaac cagttaccat gaatcattgt
     7141 acaatgctct acaatctcta agagacagag aattctctac attttatgaa agtctcaaat
     7201 atgccagagt aaaagaagtg gaagagatgt gtaagcgcag ccttgagtct gtgtattcgc
     7261 tctatcccac acttagcagg ttgcaggcca ttggagagct ggaaagcatt ggggagcttt
     7321 tctcaagatc agtcacacat agacaactct ctgaagtata tattaagtgg cagaaacact
     7381 cccagcttct caaggacagt gattttagtt ttcaggagcc tatcatggct ctacgcacag
     7441 tcattttgga gatcctgatg gaaaaggaaa tggacaactc acaaagagaa tgtattaagg
     7501 acattctcac caaacacctt gtagaactct ctatactggc cagaactttc aagaacactc
     7561 agctccctga aagggcaata tttcaaatta aacagtacaa ttcagttagc tgtggagtct
     7621 ctgagtggca gctggaagaa gcacaagtat tctgggcaaa aaaggagcag agtcttgccc
     7681 tgagtattct caagcaaatg atcaagaagt tggatgccag ctgtgcagcg aacaatccca
     7741 gcctaaaact tacatacaca gaatgtctga gggtttgtgg caactggtta gcagaaacgt
     7801 gcttagaaaa tcctgcggtc atcatgcaga cctatctaga aaaggcagta gaagttgctg
     7861 gaaattatga tggagaaagt agtgatgagc taagaaatgg aaaaatgaag gcatttctct
     7921 cattagcccg gttttcagat actcaatacc aaagaattga aaactacatg aaatcatcgg
     7981 aatttgaaaa caagcaagct ctcctgaaaa gagccaaaga ggaagtaggt ctccttaggg
     8041 aacataaaat tcagacaaac agatacacag taaaggttca gcgagagctg gagttggatg
     8101 aattagccct gcgtgcactg aaagaggatc gtaaacgctt cttatgtaaa gcagttgaaa
     8161 attatatcaa ctgcttatta agtggagaag aacatgatat gtgggtattc cgactttgtt
     8221 ccctctggct tgaaaattct ggagtttctg aagtcaatgg catgatgaag agagacggaa
     8281 tgaagattcc aacatataaa tttttgcctc ttatgtacca attggctgct agaatgggga
     8341 ccaagatgat gggaggccta ggatttcatg aagtcctcaa taatctaatc tctagaattt
     8401 caatggatca cccccatcac actttgttta ttatactggc cttagcaaat gcaaacagag
     8461 atgaatttct gactaaacca gaggtagcca gaagaagcag aataactaaa aatgtgccta
     8521 aacaaagctc tcagcttgat gaggatcgaa cagaggctgc aaatagaata atatgtacta
     8581 tcagaagtag gagacctcag atggtcagaa gtgttgaggc actttgtgat gcttatatta
     8641 tattagcaaa cttagatgcc actcagtgga agactcagag aaaaggcata aatattccag
     8701 cagaccagcc aattactaaa cttaagaatt tagaagatgt tgttgtccct actatggaaa
     8761 ttaaggtgga ccacacagga gaatatggaa atctggtgac tatacagtca tttaaagcag
     8821 aatttcgctt agcaggaggt gtaaatttac caaaaataat agattgtgta ggttccgatg
     8881 gcaaggagag gagacagctt gttaagggcc gtgatgacct gagacaagat gctgtcatgc
     8941 aacaggtctt ccagatgtgt aatacattac tgcagagaaa cacggaaact aggaagagga
     9001 aattaactat ctgtacttat aaggtggttc ccctctctca gcgaagtggt gttcttgaat
     9061 ggtgcacagg aactgtcccc attggtgaat ttcttgttaa caatgaagat ggtgctcata
     9121 aaagatacag gccaaatgat ttcagtgcct ttcagtgcca aaagaaaatg atggaggtgc
     9181 aaaaaaagtc ttttgaagag aaatatgaag tcttcatgga tgtttgccaa aattttcaac
     9241 cagttttccg ttacttctgc atggaaaaat tcttggatcc agctatttgg tttgagaagc
     9301 gattggctta tacgcgcagt gtagctactt cttctattgt tggttacata cttggacttg
     9361 gtgatagaca tgtacagaat atcttgataa atgagcagtc agcagaactt gtacatatag
     9421 atctaggtgt tgcttttgaa cagggcaaaa tccttcctac tcctgagaca gttcctttta
     9481 gactcaccag agatattgtg gatggcatgg gcattacggg tgttgaaggt gtcttcagaa
     9541 gatgctgtga gaaaaccatg gaagtgatga gaaactctca ggaaactctg ttaaccattg
     9601 tagaggtcct tctatatgat ccactctttg actggaccat gaatcctttg aaagctttgt
     9661 atttacagca gaggccggaa gatgaaactg agcttcaccc tactctgaat gcagatgacc
     9721 aagaatgcaa acgaaatctc agtgatattg accagagttt caacaaagta gctgaacgtg
     9781 tcttaatgag actacaagag aaactgaaag gagtggaaga aggcactgtg ctcagtgttg
     9841 gtggacaagt gaatttgctc atacagcagg ccatagaccc caaaaatctc agccgacttt
     9901 tcccaggatg gaaagcttgg gtgggatccg actacaaaga ccatgacggt gattataaag
     9961 atcatgacat cgactacaag gatgacgatg acaagtagtg agggcccgat atctcgacaa
    10021 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    10081 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    10141 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    10201 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    10261 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    10321 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    10381 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
    10441 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    10501 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    10561 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg
    10621 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
    10681 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    10741 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    10801 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    10861 cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc
    10921 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    10981 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    11041 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
    11101 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    11161 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11221 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11281 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    11341 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    11401 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    11461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    11521 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    11581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    11641 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    11701 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
    11761 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    11821 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    11881 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    11941 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    12001 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    12061 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    12121 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    12181 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    12241 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
    12301 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    12361 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    12421 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    12481 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    12541 gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa
    12601 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    12661 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    12721 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    12781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    12841 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    12901 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    12961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    13021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    13081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    13141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    13201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    13261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    13321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    13381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    13441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
    13501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    13561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    13621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    13681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    13741 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    13801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    13861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    13921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    13981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    14041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    14101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    14161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    14221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    14281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    14341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    14401 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    14461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    14521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    14581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    14641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    14701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    14761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    14821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    14881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    14941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    15001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    15061 cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc
    15121 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    15181 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    15241 agg
//
