LOCUS       V013918                 5914 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013918
VERSION     V013918
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1076..1438
                     /gene="VP3"
                     /label="Apoptin"
                     /note="Apoptin from Chicken anemia virus (isolate Germany
                     Cuxhaven-1). Accession#: Q99152"
     CDS             1439..2971
                     /label="VSV-G"
                     /note="vesicular stomatitis virus G glycoprotein"
     polyA_signal    complement(3028..3149)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3330..3785
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4117..4221
                     /label="AmpR promoter"
     CDS             4222..5079
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5253..5841
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattctg acactatgaa
     1081 cgctctccaa gaagatactc cacccggacc atcaacggtg ttcaggccac caacaagttc
     1141 acggccgttg gaaacccctc actgcagaga gatccggatt ggtatcgctg gaattacaat
     1201 cactctatcg ctgtgtggct gcgcgaatgc tcgcgctccc acgctaagat ctgcaactgc
     1261 ggacaattca gaaagcactg gtttcaagaa tgtgccggac ttgaggaccg atcaacccaa
     1321 gcctccctcg aagaagcgat cctgcgaccc ctccgagtac agggtaagcg agctaaaaga
     1381 aagcttgatt accactactc ccagccgacc ccgaaccgca aaaaggcgta taagactgat
     1441 gaagtgcctt ttgtacttag cctttttatt cattggggtg aattgcaagt tcaccatagt
     1501 ttttccacac aaccaaaaag gaaactggaa aaatgttcct tctaattacc attattgccc
     1561 gtcaagctca gatttaaatt ggcataatga cttaataggc acagccttac aagtcaaaat
     1621 gcccaagagt cacaaggcta ttcaagcaga cggttggatg tgtcatgctt ccaaatgggt
     1681 cactacttgt gatttccgct ggtatggacc gaagtatata acacattcca tccgatcctt
     1741 cactccatct gtagaacaat gcaaggaaag cattgaacaa acgaaacaag gaacttggct
     1801 gaatccaggc ttccctcctc aaagttgtgg atatgcaact gtgacggatg ccgaagcagt
     1861 gattgtccag gtgactcctc accatgtgct ggttgatgaa tacacaggag aatgggttga
     1921 ttcacagttc atcaacggaa aatgcagcaa ttacatatgc cccactgtcc ataactctac
     1981 aacctggcat tctgactata aggtcaaagg gctatgtgat tctaacctca tttccatgga
     2041 catcaccttc ttctcagagg acggagagct atcatccctg ggaaaggagg gcacagggtt
     2101 cagaagtaac tactttgctt atgaaactgg aggcaaggcc tgcaaaatgc aatactgcaa
     2161 gcattgggga gtcagactcc catcaggtgt ctggttcgag atggctgata aggatctctt
     2221 tgctgcagcc agattccctg aatgcccaga agggtcaagt atctctgctc catctcagac
     2281 ctcagtggat gtaagtctaa ttcaggacgt tgagaggatc ttggattatt ccctctgcca
     2341 agaaacctgg agcaaaatca gagcgggtct tccaatctct ccagtggatc tcagctatct
     2401 tgctcctaaa aacccaggaa ccggtcctgc tttcaccata atcaatggta ccctaaaata
     2461 ctttgagacc agatacatca gagtcgatat tgctgctcca atcctctcaa gaatggtcgg
     2521 aatgatcagt ggaactacca cagaaaggga actgtgggat gactgggcac catatgaaga
     2581 cgtggaaatt ggacccaatg gagttctgag gaccagttca ggatataagt ttcctttata
     2641 catgattgga catggtatgt tggactccga tcttcatctt agctcaaagg ctcaggtgtt
     2701 cgaacatcct cacattcaag acgctgcttc gcaacttcct gatgatgaga gtttattttt
     2761 tggtgatact gggctatcca aaaatccaat cgagcttgta gaaggttggt tcagtagttg
     2821 gaaaagctct attgcctctt ttttctttat catagggtta atcattggac tattcttggt
     2881 tctccgagtt ggtatccatc tttgcattaa attaaagcac accaagaaaa gacagattta
     2941 tacagacata gagatgaacc gacttggaaa gtaactcaaa tcctgcactc tagagtcgac
     3001 ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     3061 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3121 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3181 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     3241 aaatcgataa ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3301 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     3361 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3421 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3481 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3541 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3601 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3661 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3721 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3781 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     3841 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3901 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3961 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4021 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4081 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4141 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4201 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4261 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4321 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4381 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4441 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4501 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4561 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4621 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4681 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4741 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4801 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4861 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4921 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4981 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5041 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5101 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5161 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5881 tgctggcctt ttgctcacat ggctcgacag atct
//
