LOCUS       V027007                 8866 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027007
VERSION     V027007
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3525
                     /label="AP2A2(NM_001242837)"
                     /note="AP2A2(NM_001242837)"
                     /gene="AP2A2"
     CDS             3532..3558
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3562..3588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3592..3618
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3642..4230
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4296..4344
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4546..4974
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4988..5317
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5384..6178
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6354..6475
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6524..6540)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6548..6564
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6572..6602)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6617..6638
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6926..7514)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7685..8545)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8546..8650)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcc ggccgtgtcc
      721 aagggggacg ggatgcgggg cctggcggtc ttcatctcgg atatccgcaa ctgtaaaagt
      781 aaagaagcag aaataaaaag gataaacaag gaactggcaa atatcagatc aaaatttaaa
      841 ggtgacaagg ctcttgatgg ctatagtaaa aaaaagtacg tctgcaagtt gctcttcatc
      901 tttctccttg gtcatgacat tgactttgga cacatggagg ctgtgaacct gctgagttca
      961 aacagataca cggaaaagca gatcggctac cttttcatct ctgtgttggt gaactcaaac
     1021 agtgagctga tccgcctgat caacaacgcc atcaagaatg acctggccag ccgcaacccc
     1081 accttcatgg gcctggccct gcactgcatc gccagcgtgg gcagccggga gatggccgag
     1141 gccttcgccg gggagatccc taaggtcctc gtagccggag acactatgga cagcgtgaag
     1201 cagagcgcgg ccctgtgctt gctgcgcctg tacaggacgt cccccgatct tgtccccatg
     1261 ggcgactgga catcccgagt ggtgcacctg ctcaatgacc agcacttggg tgtggtaact
     1321 gcagccacaa gtctgatcac cactttagca cagaagaacc cagaagagtt taaaacctcc
     1381 gtgtctctgg ctgtctctag gctaagcaga atcgtgacgt ctgcatccac agatctccag
     1441 gattacactt actattttgt cccggctccc tggctgtctg tcaaactgct gagactgctg
     1501 cagtgctacc cacccccaga agaccctgca gtgcgaggcc gcctgactga gtgcctggag
     1561 accatcctga acaaagccca agaaccgccc aagtcgaaga aggtccagca ctccaacgcg
     1621 aagaatgccg tgctcttcga ggccatcagc ttaatcattc accatgacag tgagccgaac
     1681 ctgctcgtcc gtgcctgcaa ccagttgggc cagtttctgc agcaccgcga gaccaacctg
     1741 cgctacctgg ccctggagag catgtgcacg ctggccagct ctgagttctc ccatgaggct
     1801 gtcaagacgc acatcgagac ggtcatcaac gccctgaaga ctgagcggga cgtgagcgtg
     1861 cggcagcggg ccgtggacct cctctacgcc atgtgcgacc gcagcaacgc cccacagatc
     1921 gtggccgaga tgctgagcta tctggagaca gctgactact ccatccgaga agagattgtg
     1981 ctgaaggtcg ccatcctggc tgagaagtac gcggtggact acacctggta tgtggatacc
     2041 atcttgaact tgatccgaat tgctggtgat tacgtgagtg aagaggtgtg gtaccgagtc
     2101 attcagatcg tcatcaaccg ggacgacgtg cagggctacg cggccaagac tgtgttcgag
     2161 gctcttcagg ctcccgcgtg ccacgagaac ctggtcaaag tgggcggcta catcctgggg
     2221 gagtttggaa acttgatagc tggagacccg agatccagcc cgctgatcca gttccacctg
     2281 ctgcactcca agttccacct gtgcagcgtc cccacccgcg cgctgctcct gtccacctac
     2341 atcaagttcg tgaacctctt cccggaggtg aagcccacca tccaggacgt gctgcgcagc
     2401 gacagccagc tcaggaacgc agacgtggag ctgcagcagc gtgctgtgga gtacctgcgg
     2461 ctcagcaccg tggccagcac cgacattctg gcgaccgtgc tggaggagat gcccccattc
     2521 ccggagcggg agtcctccat cttggcaaag ctcaagaaga agaagggccc cagcacggtg
     2581 acagacctgg aggacaccaa gcgggacagg agtgtggacg tgaacggggg tcctgagcct
     2641 gccccagcca gtaccagcgc cgtgtctacg ccttctccgt cggcagacct gctgggtctc
     2701 ggggctgccc cccctgcccc cgcgggcccc ccaccctcct ccggcggcag cgggctgctc
     2761 gtggacgtgt tctcagactc ggcctctgtg gtcgcgcctc tcgctcctgg ctccgaagac
     2821 aactttgcca ggtttgtttg taaaaacaat ggtgtgttgt ttgaaaacca gctgcttcaa
     2881 attggactta agtctgaatt tcggcagaat ttaggtcgga tgtttatctt ttatggtaat
     2941 aagacctcca cgcagttcct aaactttacc ccaacactaa tctgttcaga cgaccttcag
     3001 cctaacctga acctgcagac caagcccgtg gacccgaccg tggagggggg cgcgcaggtg
     3061 cagcaggtgg tcaacataga gtgcgtgtcc gacttcacgg aggcgccagt cctcaacatt
     3121 cagttcaggt atgggggcac cttccagaac gtgtctgtgc agctgcccat cactctcaac
     3181 aaattcttcc agccgacaga aatggcttct caggatttct ttcaacgttg gaagcagttg
     3241 agcaatccac agcaggaagt gcagaacatc ttcaaagcaa agcacccaat ggacacagaa
     3301 gtcaccaaag ccaagatcat tggatttggt tctgcacttc ttgaagaagt tgatcctaat
     3361 cctgcgaatt tcgtgggagc tggaatcatc cacacgaaaa ccacccagat tggatgcctg
     3421 ctgcgcttgg agccgaacct gcaagcccag atgtaccggc tcacgctgcg cacaagtaag
     3481 gaagccgttt ctcagagatt atgtgaattg ctctcagcgc agtttctcga gtacccatac
     3541 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat
     3601 gatgtcccag actacgcata gtgagggccc gatatctcga caatcaacct ctggattaca
     3661 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3721 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3781 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3841 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3901 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3961 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4021 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     4081 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4141 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4201 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg
     4261 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     4321 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     4381 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     4441 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     4501 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4681 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
     4741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
     4921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4981 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5041 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5161 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5221 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5281 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5341 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5401 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5461 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5521 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5581 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5641 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5701 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5761 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5821 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5881 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     5941 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6001 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6061 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6121 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6181 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6241 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6301 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6361 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6421 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6481 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     7141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8701 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8761 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8821 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg
//
