LOCUS       V015213                 7508 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015213
VERSION     V015213
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             683..2155
                     /gene="ANGPTL1"
                     /label="Angiopoietin-related protein 1"
                     /note="Angiopoietin-related protein 1 from Homo sapiens.
                     Accession#: O95841"
     CDS             2195..2260
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    2284..2872
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    2938..2986
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3188..3616
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3630..3959
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4026..4817
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4996..5129
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5166..5182)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5190..5206)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5214..5244)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5259..5280)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5568..6156)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6330..7187)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7188..7292)
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgaagac ttttacctgg accctaggtg tgctattctt
      721 cctactagtg gacactggac attgcagagg tggacaattc aaaattaaaa aaataaacca
      781 gagaagatac cctcgtgcca cagatggtaa agaggaagca aagaaatgtg catacacatt
      841 cctggtacct gaacaaagaa taacagggcc aatctgtgtc aacaccaagg ggcaagatgc
      901 aagtaccatt aaagacatga tcaccaggat ggaccttgaa aacctgaagg atgtgctctc
      961 caggcagaag cgggagatag atgttctgca actggtggtg gatgtagatg gaaacattgt
     1021 gaatgaggta aagctgctga gaaaggaaag ccgtaacatg aactctcgtg ttactcaact
     1081 ctatatgcaa ttattacatg agattatccg taagagggat aattcacttg aactttccca
     1141 actggaaaac aaaatcctca atgtcaccac agaaatgttg aagatggcaa caagatacag
     1201 ggaactagag gtgaaatacg cttccttgac tgatcttgtc aataaccaat ctgtgatgat
     1261 cactttgttg gaagaacagt gcttgaggat attttcccga caagacaccc atgtgtctcc
     1321 cccacttgtc caggtggtgc cacaacatat tcctaacagc caacagtata ctcctggtct
     1381 gctgggaggt aacgagattc agagggatcc aggttatccc agagatttaa tgccaccacc
     1441 tgatctggca acttctccca ccaaaagccc tttcaagata ccaccggtaa ctttcatcaa
     1501 tgaaggacca ttcaaagact gtcagcaagc aaaagaagct gggcattcgg tcagtgggat
     1561 ttatatgatt aaacctgaaa acagcaatgg accaatgcag ttatggtgtg aaaacagttt
     1621 ggaccctggg ggttggactg ttattcagaa aagaacagac ggctctgtca acttcttcag
     1681 aaattgggaa aattataaga aagggtttgg aaacattgac ggagaatact ggcttggact
     1741 ggaaaatatc tatatgctta gcaatcaaga taattacaag ttattgattg aattagaaga
     1801 ctggagtgat aaaaaagtct atgcagaata cagcagcttt cgtctggaac ctgaaagtga
     1861 attctataga ctgcgcctgg gaacttacca gggaaatgca ggggattcta tgatgtggca
     1921 taatggtaaa caattcacca cactggacag agataaagat atgtatgcag gaaactgcgc
     1981 ccactttcat aaaggaggct ggtggtacaa tgcctgtgca cattctaacc taaatggagt
     2041 atggtacaga ggaggccatt acagaagcaa gcaccaagat ggaattttct gggccgaata
     2101 cagaggcggg tcatactcct taagagcagt tcagatgatg atcaagccta ttgacgaatt
     2161 ctgcagatat ccagcacagt ggcggccgct cgaggactac aaagaccatg acggtgatta
     2221 taaagatcat gacatcgact acaaggatga cgatgacaag tagtgagggc ccgatatctc
     2281 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2341 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2401 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2461 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2521 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2581 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2641 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     2701 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2761 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     2821 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaaac
     2881 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     2941 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
     3001 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     3061 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     3121 gccaacgtcg gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg
     3181 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3241 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3301 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc
     3361 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3421 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3481 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3541 gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3601 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3661 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3721 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3781 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3841 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3901 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3961 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4021 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4081 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4141 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4201 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4261 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4321 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4381 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4441 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4501 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     4561 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4621 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4681 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4741 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4801 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg
     4861 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4921 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4981 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5101 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5221 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5341 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5401 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5461 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5521 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5761 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6001 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6061 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6121 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6241 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6301 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6361 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6421 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6481 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6541 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6601 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6661 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6721 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6781 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6841 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6901 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6961 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7021 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7081 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7141 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7201 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7261 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7321 gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg
     7381 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     7441 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     7501 tgcttagg
//
