LOCUS V024368 6679 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024368 VERSION V024368 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1314 /label="AKIRIN2(NM_018064)" /note="AKIRIN2(NM_018064)" /gene="AKIRIN2" CDS 1321..1338 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" CDS 1345..1371 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1375..1401 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1405..1431 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1455..2043 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2109..2157 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2359..2787 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2801..3130 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3197..3991 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4167..4288 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4337..4353) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4361..4377 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4385..4415) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4430..4451 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4739..5327) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5498..6358) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6359..6463) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc gtgcggagcc 721 actctgaaaa ggactctgga tttcgacccg ctgttgagcc cggcgtcccc gaagcgcagg 781 cgatgtgcgc cattgtcggc gcccacctcg gccgctgcct ccccgttgtc ggcggccgcg 841 gccaccgccg cctccttctc cgctgcggcc gcctcgccgc agaagtatct ccgaatggag 901 ccatccccct tcggcgacgt ctcctcccgc ctcaccacag aacaaattct gtacaacata 961 aaacaagagt ataaacgaat gcagaagaga agacatttag aaacgagttt ccaacagaca 1021 gatccgtgtt gtacttctga tgcacagcca catgcatttc tcctcagtgg accagcttca 1081 ccagggactt catctgcagc atcctcacca ttaaaaaaag aacagccctt atttactcta 1141 cggcaggttg ggatgatctg tgaacgtttg ttgaaagaac gtgaagagaa agttcgagaa 1201 gaatatgaag aaatattgaa cacaaaactt gcagaacaat atgatgcgtt tgtgaagttt 1261 acgcatgatc aaataatgcg acgatatgga gaacagcctg ctagctatgt ttcatctaga 1321 catcaccatc atcaccatct cgagtaccca tacgacgtac ctgattatgc aacttatcct 1381 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atagtgaggg 1441 cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 1501 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 1561 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 1621 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 1681 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 1741 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 1801 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 1861 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 1921 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 1981 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 2041 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 2101 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata 2161 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 2221 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 2281 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg 2341 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 2401 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 2461 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc 2521 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 2581 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 2641 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 2701 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc 2761 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 2821 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 2881 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 2941 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 3001 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 3061 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 3121 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 3181 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 3241 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 3301 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 3361 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 3421 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 3481 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 3541 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 3601 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 3661 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 3721 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 3781 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 3841 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 3901 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 3961 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg 4021 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 4081 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 4141 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 4201 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 4261 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 4321 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 4381 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 4441 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 4501 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 4561 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 4621 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 4681 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 4741 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 4801 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 4861 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 4921 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 4981 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 5041 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 5101 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 5161 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 5221 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 5281 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 5341 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 5401 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 5461 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 5521 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 5581 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 5641 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 5701 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 5761 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 5821 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 5881 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 5941 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 6001 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 6061 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 6121 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 6181 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 6241 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 6301 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 6361 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 6421 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 6481 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac 6541 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt 6601 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 6661 catgaagaat ctgcttagg //