LOCUS       V044100                 8593 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044100
VERSION     V044100
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3249
                     /label="AHR(NM_001621)"
                     /note="AHR(NM_001621)"
                     /gene="AHR"
     CDS             3256..3345
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    3369..3957
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4023..4071
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4273..4701
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4715..5044
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5111..5905
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6081..6202
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6251..6267)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6275..6291
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6299..6329)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6344..6365
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6653..7241)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7412..8272)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8273..8377)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgaa cagcagcagc
      721 gccaacatca cctacgccag tcgcaagcgg cggaagccgg tgcagaaaac agtaaagcca
      781 atcccagctg aaggaatcaa gtcaaatcct tccaagcggc atagagaccg acttaataca
      841 gagttggacc gtttggctag cctgctgcct ttcccacaag atgttattaa taagttggac
      901 aaactttcag ttcttaggct cagcgtcagt tacctgagag ccaagagctt ctttgatgtt
      961 gcattaaaat cctcccctac tgaaagaaac ggaggccagg ataactgtag agcagcaaat
     1021 ttcagagaag gcctgaactt acaagaagga gaattcttat tacaggctct gaatggcttt
     1081 gtattagttg tcactacaga tgctttggtc ttttatgctt cttctactat acaagattat
     1141 ctagggtttc agcagtctga tgtcatacat cagagtgtat atgaacttat ccataccgaa
     1201 gaccgagctg aatttcagcg tcagctacac tgggcattaa atccttctca gtgtacagag
     1261 tctggacaag gaattgaaga agccactggt ctcccccaga cagtagtctg ttataaccca
     1321 gaccagattc ctccagaaaa ctctccttta atggagaggt gcttcatatg tcgtctaagg
     1381 tgtctgctgg ataattcatc tggttttctg gcaatgaatt tccaagggaa gttaaagtat
     1441 cttcatggac agaaaaagaa agggaaagat ggatcaatac ttccacctca gttggctttg
     1501 tttgcgatag ctactccact tcagccacca tccatacttg aaatccggac caaaaatttt
     1561 atctttagaa ccaaacacaa actagacttc acacctattg gttgtgatgc caaaggaaga
     1621 attgttttag gatatactga agcagagctg tgcacgagag gctcaggtta tcagtttatt
     1681 catgcagctg atatgcttta ttgtgccgag tcccatatcc gaatgattaa gactggagaa
     1741 agtggcatga tagttttccg gcttcttaca aaaaacaacc gatggacttg ggtccagtct
     1801 aatgcacgcc tgctttataa aaatggaaga ccagattata tcattgtaac tcagagacca
     1861 ctaacagatg aggaaggaac agagcattta cgaaaacgaa atacgaagtt gccttttatg
     1921 tttaccactg gagaagctgt gttgtatgag gcaaccaacc cttttcctgc cataatggat
     1981 cccttaccac taaggactaa aaatggcact agtggaaaag actctgctac cacatccact
     2041 ctaagcaagg actctctcaa tcctagttcc ctcctggctg ccatgatgca acaagatgag
     2101 tctatttatc tctatcctgc ttcaagtact tcaagtactg caccttttga aaacaacttt
     2161 ttcaacgaat ctatgaatga atgcagaaat tggcaagata atactgcacc gatgggaaat
     2221 gatactatcc tgaaacatga gcaaattgac cagcctcagg atgtgaactc atttgctgga
     2281 ggtcacccag ggctctttca agatagtaaa aacagtgact tgtacagcat aatgaaaaac
     2341 ctaggcattg attttgaaga catcagacac atgcagaatg aaaaattttt cagaaatgat
     2401 ttttctggtg aggttgactt cagagacatt gacttaacgg atgaaatcct gacgtatgtc
     2461 caagattctt taagtaagtc tcccttcata ccttcagatt atcaacagca acagtccttg
     2521 gctctgaact caagctgtat ggtacaggaa cacctacatc tagaacagca acagcaacat
     2581 caccaaaagc aagtagtagt ggagccacag caacagctgt gtcagaagat gaagcacatg
     2641 caagttaatg gcatgtttga aaattggaac tctaaccaat tcgtgccttt caattgtcca
     2701 cagcaagacc cacaacaata taatgtcttt acagacttac atgggatcag tcaagagttc
     2761 ccctacaaat ctgaaatgga ttctatgcct tatacacaga actttatttc ctgtaatcag
     2821 cctgtattac cacaacattc caaatgtaca gagctggact accctatggg gagttttgaa
     2881 ccatccccat accccactac ttctagttta gaagattttg tcacttgttt acaacttcct
     2941 gaaaaccaaa agcatggatt aaatccacag tcagccataa taactcctca gacatgttat
     3001 gctggggccg tgtcgatgta tcagtgccag ccagaacctc agcacaccca cgtgggtcag
     3061 atgcagtaca atccagtact gccaggccaa caggcatttt taaacaagtt tcagaatgga
     3121 gttttaaatg aaacatatcc agctgaatta aataacataa ataacactca gactaccaca
     3181 catcttcagc cacttcatca tccgtcagaa gccagacctt ttcctgattt gacatccagt
     3241 ggattcctgc tcgaggaaca aaaactaata tcagaggaag atttggaaca gaaactaatc
     3301 agtgaagaag atttagagca aaagttaatt tccgaggagg acttatagtg agggcccgat
     3361 atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3421 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3481 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3541 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3601 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3661 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3721 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     3781 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3841 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3901 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     3961 gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4021 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     4081 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     4141 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     4201 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta
     4261 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4321 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4381 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag
     4441 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4501 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4561 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4621 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt
     4681 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4741 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4801 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4861 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4921 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4981 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5041 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5101 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5161 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5221 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5281 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5341 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5401 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5461 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5521 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5581 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5641 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5701 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5761 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5821 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5881 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag
     5941 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6001 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6061 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6121 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6181 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6241 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6301 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6361 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6421 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6481 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6541 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6841 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6901 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6961 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7021 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7081 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7141 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7201 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7261 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7321 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7381 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7441 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7501 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7561 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7621 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7681 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7741 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7801 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7861 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7921 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7981 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8041 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8101 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8161 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8221 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8281 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8341 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8401 ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg
     8461 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     8521 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     8581 gaatctgctt agg
//
