LOCUS       V013764                 6518 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013764
VERSION     V013764
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             627..2375
                     /gene="gox"
                     /label="Glucose oxidase"
                     /note="Glucose oxidase from Aspergillus niger. Accession#:
                     P13006"
     CDS             2409..3116
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    3117..3182
                     /label="MCS"
                     /note="multiple cloning site"
     polyA_signal    3306..3427
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3434..3889)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3916..4020
                     /label="AmpR promoter"
     promoter        4022..4379
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4414..5205
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5440..5487
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5816..6404
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgagca acggcatcga ggccagcctg ctgaccgacc
      661 ccaaggacgt gagcggccgc accgtggact acatcatcgc cggcggcggc ctgaccggcc
      721 tgaccaccgc cgcccgcctg accgagaacc ccaacatcag cgtgctggtg atcgagagcg
      781 gcagctacga gagcgaccgc ggccccatca tcgaggacct gaacgcctac ggcgacatct
      841 tcggcagcag cgtggaccac gcctacgaga ccgtggagct ggccaccaac aaccagaccg
      901 ccctgatccg cagcggcaac ggcctgggcg gcagcaccct ggtgaacggc ggcacctgga
      961 cccgccccca caaggcccag gtggacagct gggagaccgt gttcggcaac gagggctgga
     1021 actgggacaa cgtggccgcc tacagcctgc aggccgagcg cgcccgcgcc cccaacgcca
     1081 agcagatcgc cgccggccac tacttcaacg ccagctgcca cggcgtgaac ggcaccgtgc
     1141 acgccggccc ccgcgacacc ggcgacgact acagccccat cgtgaaggcc ctgatgagcg
     1201 ccgtggagga ccgcggcgtg cccaccaaga aggacttcgg ctgcggcgac ccccacggcg
     1261 tgagcatgtt ccccaacacc ctgcacgagg accaggtgcg cagcgacgcc gcccgcgagt
     1321 ggctgctgcc caactaccag cgccccaacc tgcaggtgct gaccggccag tacgtgggca
     1381 aggtgctgct gagccagaac ggcaccaccc cccgcgccgt gggcgtggag ttcggcaccc
     1441 acaagggcaa cacccacaac gtgtacgcca agcacgaggt gctgctggcc gccggcagcg
     1501 ccgtgagccc caccatcctg gagtacagcg gcatcggcat gaagagcatc ctggagcccc
     1561 tgggcatcga caccgtggtg gacctgcccg tgggcctgaa cctgcaggac cagaccaccg
     1621 ccaccgtgcg cagccgcatc accagcgccg gcgccggcca gggccaggcc gcctggttcg
     1681 ccaccttcaa cgagaccttc ggcgactaca gcgagaaggc ccacgagctg ctgaacacca
     1741 agctggagca gtgggccgag gaggccgtgg cccgcggcgg cttccacaac accaccgccc
     1801 tgctgatcca gtacgagaac taccgcgact ggatcgtgaa ccacaacgtg gcctacagcg
     1861 agctgttcct ggacaccgcc ggcgtggcca gcttcgacgt gtgggacctg ctgcccttca
     1921 cccgcggcta cgtgcacatc ctggacaagg acccctacct gcaccacttc gcctacgacc
     1981 cccagtactt cctgaacgag ctggacctgc tgggccaggc cgccgccacc cagctggccc
     2041 gcaacatcag caacagcggc gccatgcaga cctacttcgc cggcgagacc atccccggcg
     2101 acaacctggc ctacgacgcc gacctgagcg cctggaccga gtacatcccc taccacttcc
     2161 gccccaacta ccacggcgtg ggcacctgca gcatgatgcc caaggagatg ggcggcgtgg
     2221 tggacaacgc cgcccgcgtg tacggcgtgc agggcctgcg cgtgatcgac ggcagcatcc
     2281 cccccaccca gatgagcagc cacgtgatga ccgtgttcta cgccatggcc ctgaagatca
     2341 gcgacgccat cctggaggac tacgccagca tgcagggagg agggggaagc gatccaccgg
     2401 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     2461 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2521 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2581 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     2641 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     2701 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2761 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2821 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2881 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2941 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     3001 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     3061 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg
     3121 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
     3181 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     3241 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3301 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3421 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3481 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3541 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3601 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3661 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3721 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3781 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3841 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3901 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3961 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4021 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4081 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4141 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4201 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4261 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4321 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4381 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4441 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4501 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4561 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4621 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4681 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4741 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4801 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4861 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4921 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4981 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5041 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5101 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5161 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5221 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5281 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5341 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5401 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5461 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5521 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5581 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5641 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5701 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5941 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6481 cccctgattc tgtggataac cgtattaccg ccatgcat
//
