LOCUS       Exported               12455 bp DNA     circular SYN 03-SEP-2024
DEFINITION  Gateway(R)-compatible plant transformation vector with YFP and HA 
            C-terminal tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 12455)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12455)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12455)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2006"; volume: "45"; pages: "616-29"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     The sequence was corrected by inserting a G at position 8562.
FEATURES             Location/Qualifiers
     source          1..12455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2663..2782
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2812..2842
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2896..3552
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3897..4199
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFLGI"
     protein_bind    complement(4243..4367)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4375..5088
                     /codon_start=1
                     /label=EYFP
                     /note="enhanced YFP"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    5089..5154
                     /label=MCS
                     /note="multiple cloning site"
     CDS             5167..5193
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     terminator      5225..5932
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    6176..6200
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7501..8127
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8559..9629
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9698..9892
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10236..10376
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10562..11150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11240..12031)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     2761 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
     2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     3541 cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat
     3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc ttgggaatat
     4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     4261 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc tagggtgagc
     4381 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4441 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4501 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4561 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     4621 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4681 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4741 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4801 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     4861 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     4921 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     4981 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5041 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
     5101 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
     5161 tctagatacc catacgatgt tccagattac gcttaattaa gacccgggac tagtccctag
     5221 agtcctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc
     5281 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg
     5341 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt
     5401 tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat
     5461 tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg
     5521 ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact
     5581 gattacataa atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac
     5641 cgagcggcga actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg
     5701 gtgagattcc ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt
     5761 caacccggtc cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat
     5821 ttttttggtg tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa
     5881 tcgttgggcg ggtccagggc gaattttgcg acaacatgtc gaggctcagc aggacctgca
     5941 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6001 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6061 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     6121 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     6181 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     6241 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     6301 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     6361 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     6421 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     6481 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     6541 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     6601 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     6661 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     6721 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     6781 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     6841 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     6901 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     6961 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     7021 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     7081 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     7141 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     7201 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     7261 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     7321 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     7381 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     7441 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     7501 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     7561 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     7621 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     7681 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     7741 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     7801 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     7861 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     7921 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     7981 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     8041 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     8101 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     8161 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     8221 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     8281 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     8341 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     8401 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     8461 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     8521 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     8581 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     8641 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     8701 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     8761 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     8821 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     8881 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     8941 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     9001 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     9061 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     9121 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     9181 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     9241 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     9301 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     9361 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     9421 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     9481 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     9541 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     9601 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     9661 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     9721 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     9781 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     9841 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     9901 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     9961 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
    10021 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
    10081 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    10141 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    10201 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
    10261 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10321 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    10381 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    10441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    10501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10981 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    11041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    11101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    11161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    11221 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    11281 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    11341 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    11401 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    11461 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    11521 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    11581 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    11641 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    11701 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    11761 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    11821 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    11881 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    11941 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    12001 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    12061 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    12121 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    12181 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    12241 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    12301 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    12361 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    12421 ccgagctgcc ggtcggggag ctgttggctg gctgg
//
