LOCUS       40924_18991        2056 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Blue/white screening vector with an ampicillin resistance marker for
            high efficiency blunt end cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..2056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..48
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    56..72
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..96
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(127..143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      324..355
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        356..458
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             459..1316
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1343..1370
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1382..1969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1991..2020)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
       61 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgtcgac
      121 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctaat aaatttaaat
      301 catacctgac ctccatagca gaaagtcaaa agcctccgac cggaggcttt tgacttgatc
      361 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      421 ttgagttatc gagattttca ggagctaagg aagctaaaat gagtattcaa catttccgtg
      481 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      541 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      601 atctcaacag cggtaagatc cttgagagtt tacgccccga agaacgtttt ccaatgatga
      661 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      721 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      781 aaaagcatct cacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      841 gtgataacac tgcggccaac ttacttctgg caacgatcgg aggaccgaag gagctaaccg
      901 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
      961 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1021 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1081 ggatggaggc ggataaagtt gcaggatcac ttctgcgctc ggccctcccg gctggctggt
     1141 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1201 ggccagatgg taagccctcc cgcatcgtag ttatctacac gacggggagt caggcaacta
     1261 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaat
     1321 gagggcccaa atgtaatcac ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt
     1381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgatgctca agtcagaggt
     1441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1561 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1801 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1861 cctcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1981 gattttctac cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgcagtcca
     2041 gttacgctgg agtctg
//
