LOCUS       V006399                 6358 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006399
VERSION     V006399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6358)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6358)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EYFP"
                     /note="enhanced YFP"
     CDS             1363..3015
                     /gene="RELA"
                     /label="Transcription factor p65"
                     /note="Transcription factor p65 from Homo sapiens.
                     Accession#: Q04206"
     polyA_signal    3146..3267
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3274..3729)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3756..3860
                     /label="AmpR promoter"
     promoter        3862..4219
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4254..5045
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5280..5327
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5656..6244
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcca ccatggacga actgttcccc
     1381 ctcatcttcc cggcagagcc agcccaggcc tctggcccct atgtggagat cattgagcag
     1441 cccaagcagc ggggcatgcg cttccgctac aagtgcgagg ggcgctccgc gggcagcatc
     1501 ccaggcgaga ggagcacaga taccaccaag acccacccca ccatcaagat caatggctac
     1561 acaggaccag ggacagtgcg catctccctg gtcaccaagg accctcctca ccggcctcac
     1621 ccccacgagc ttgtaggaaa ggactgccgg gatggcttct atgaggctga gctctgcccg
     1681 gaccgctgca tccacagttt ccagaacctg ggaatccagt gtgtgaagaa gcgggacctg
     1741 gagcaggcta tcagtcagcg catccagacc aacaacaacc ccttccaagt tcctatagaa
     1801 gagcagcgtg gggactacga cctgaatgct gtgcggctct gcttccaggt gacagtgcgg
     1861 gacccatcag gcaggcccct ccgcctgccg cctgtccttc ctcatcccat ctttgacaat
     1921 cgtgccccca acactgccga gctcaagatc tgccgagtga accgaaactc tggcagctgc
     1981 ctcggtgggg atgagatctt cctactgtgt gacaaggtgc agaaagagga cattgaggtg
     2041 tatttcacgg gaccaggctg ggaggcccga ggctcctttt cgcaagctga tgtgcaccga
     2101 caagtggcca ttgtgttccg gacccctccc tacgcagacc ccagcctgca ggctcctgtg
     2161 cgtgtctcca tgcagctgcg gcggccttcc gaccgggagc tcagtgagcc catggaattc
     2221 cagtacctgc cagatacaga cgatcgtcac cggattgagg agaaacgtaa aaggacatat
     2281 gagaccttca agagcatcat gaagaagagt cctttcagcg gacccaccga cccccggcct
     2341 ccacctcgac gcattgctgt gccttcccgc agctcagctt ctgtccccaa gccagcaccc
     2401 cagccctatc cctttacgtc atccctgagc accatcaact atgatgagtt tcccaccatg
     2461 gtgtttcctt ctgggcagat cagccaggcc tcggccttgg ccccggcccc tccccaagtc
     2521 ctgccccagg ctccagcccc tgcccctgct ccagccatgg tatcagctct ggcccaggcc
     2581 ccagcccctg tcccagtcct agccccaggc cctcctcagg ctgtggcccc acctgccccc
     2641 aagcccaccc aggctgggga aggaacgctg tcagaggccc tgctgcagct gcagtttgat
     2701 gatgaagacc tgggggcctt gcttggcaac agcacagacc cagctgtgtt cacagacctg
     2761 gcatccgtcg acaactccga gtttcagcag ctgctgaacc agggcatacc tgtggccccc
     2821 cacacaactg agcccatgct gatggagtac cctgaggcta taactcgcct agtgacaggg
     2881 gcccagaggc cccccgaccc agctcctgct ccactggggg ccccggggct ccccaatggc
     2941 ctcctttcag gagatgaaga cttctcctcc attgcggaca tggacttctc agccctgctg
     3001 agtcagatca gctccgggat ccaccggatc tagataactg atcataatca gccataccac
     3061 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3121 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3181 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3241 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     3301 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3361 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3421 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3481 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3541 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3601 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3661 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3721 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3841 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     4261 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4321 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4381 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4441 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4501 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4561 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4621 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4681 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4741 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4801 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4861 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4921 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4981 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5041 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5101 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5161 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5221 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     5281 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     5341 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     5401 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5461 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     5521 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5581 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5761 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
