LOCUS       V006400                 6317 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006400
VERSION     V006400
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6317)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6317)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1287
                     /label="DsRed-Monomer"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
     CDS             1321..2973
                     /gene="RELA"
                     /label="Transcription factor p65"
                     /note="Transcription factor p65 from Homo sapiens.
                     Accession#: Q04206"
     polyA_signal    3105..3226
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3233..3688)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3715..3819
                     /label="AmpR promoter"
     promoter        3821..4178
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4213..5004
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5239..5286
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5615..6203
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg
      661 cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag
      721 ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc
      781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc
      841 gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc
      901 atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc
      961 accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg
     1021 cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg
     1081 ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac
     1141 ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac
     1201 tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac
     1261 gccgaggccc gccactccgg ctcccagtcc ggactcagat ctcgagctca agcttccacc
     1321 atggacgaac tgttccccct catcttcccg gcagagccag cccaggcctc tggcccctat
     1381 gtggagatca ttgagcagcc caagcagcgg ggcatgcgct tccgctacaa gtgcgagggg
     1441 cgctccgcgg gcagcatccc aggcgagagg agcacagata ccaccaagac ccaccccacc
     1501 atcaagatca atggctacac aggaccaggg acagtgcgca tctccctggt caccaaggac
     1561 cctcctcacc ggcctcaccc ccacgagctt gtaggaaagg actgccggga tggcttctat
     1621 gaggctgagc tctgcccgga ccgctgcatc cacagtttcc agaacctggg aatccagtgt
     1681 gtgaagaagc gggacctgga gcaggctatc agtcagcgca tccagaccaa caacaacccc
     1741 ttccaagttc ctatagaaga gcagcgtggg gactacgacc tgaatgctgt gcggctctgc
     1801 ttccaggtga cagtgcggga cccatcaggc aggcccctcc gcctgccgcc tgtccttcct
     1861 catcccatct ttgacaatcg tgcccccaac actgccgagc tcaagatctg ccgagtgaac
     1921 cgaaactctg gcagctgcct cggtggggat gagatcttcc tactgtgtga caaggtgcag
     1981 aaagaggaca ttgaggtgta tttcacggga ccaggctggg aggcccgagg ctccttttcg
     2041 caagctgatg tgcaccgaca agtggccatt gtgttccgga cccctcccta cgcagacccc
     2101 agcctgcagg ctcctgtgcg tgtctccatg cagctgcggc ggccttccga ccgggagctc
     2161 agtgagccca tggaattcca gtacctgcca gatacagacg atcgtcaccg gattgaggag
     2221 aaacgtaaaa ggacatatga gaccttcaag agcatcatga agaagagtcc tttcagcgga
     2281 cccaccgacc cccggcctcc acctcgacgc attgctgtgc cttcccgcag ctcagcttct
     2341 gtccccaagc cagcacccca gccctatccc tttacgtcat ccctgagcac catcaactat
     2401 gatgagtttc ccaccatggt gtttccttct gggcagatca gccaggcctc ggccttggcc
     2461 ccggcccctc cccaagtcct gccccaggct ccagcccctg cccctgctcc agccatggta
     2521 tcagctctgg cccaggcccc agcccctgtc ccagtcctag ccccaggccc tcctcaggct
     2581 gtggccccac ctgcccccaa gcccacccag gctggggaag gaacgctgtc agaggccctg
     2641 ctgcagctgc agtttgatga tgaagacctg ggggccttgc ttggcaacag cacagaccca
     2701 gctgtgttca cagacctggc atccgtcgac aactccgagt ttcagcagct gctgaaccag
     2761 ggcatacctg tggcccccca cacaactgag cccatgctga tggagtaccc tgaggctata
     2821 actcgcctag tgacaggggc ccagaggccc cccgacccag ctcctgctcc actgggggcc
     2881 ccggggctcc ccaatggcct cctttcagga gatgaagact tctcctccat tgcggacatg
     2941 gacttctcag ccctgctgag tcagatcagc tccttggatc caccggatct agataactga
     3001 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3061 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     3121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
     3241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3661 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3781 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     3841 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3901 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3961 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4021 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4081 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4141 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     4201 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4261 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4321 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4381 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4441 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4501 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4561 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4621 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4681 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4741 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4801 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4861 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4921 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4981 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5041 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5101 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5161 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     5221 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     5281 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     5341 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     5401 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     5461 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5521 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5581 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5641 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5701 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5761 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5821 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5881 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5941 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6001 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6061 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6181 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6241 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6301 gtattaccgc catgcat
//
