LOCUS       40924_18911        9917 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pEXPR64, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Siciliano V.
  TITLE     Engineering modular intracellular protein sensor-actuator devices
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   Siciliano V.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2018) Biological Engineering, Massachusetts 
            Institute of Technology, 500 Tech Square, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 9917)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-APR-2018) Biological Engineering, Massachusetts Institute of 
            Technology, 500 Tech Square, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(984..1841)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1842..1946)
                     /label=AmpR promoter
     rep_origin      complement(1972..2427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2569..2585
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2595..2613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2628..3431
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3510..3535
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    4207..4227
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        4252..5424
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     protein_bind    5446..5470
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5505..5514
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5619..5648
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6102..6797
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     CDS             7179..7205
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             7212..7232
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             7239..7472
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             7479..7916
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     protein_bind    complement(7950..7974)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8129..8184
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8545..8561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8569..8585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8593..8623)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    8832..9668
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(9698..9716)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9737..9753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9761..9777)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9785..9815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9830..9851)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
       61 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     1981 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2041 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2101 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2161 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2221 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2281 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2341 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2401 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     2461 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     2521 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     2581 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctgc tttctctgac
     2641 cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc
     2701 tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt cctccactcc
     2761 ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg agcacttcct
     2821 tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct gggtcctctc
     2881 cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg ttcccttttc
     2941 cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct ccgacggatg
     3001 tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt tttctggaca
     3061 accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg ctttctttgc
     3121 ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat ttgggcagct
     3181 cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg tcatggcatc
     3241 ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc tatgtccact
     3301 tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg accaccttat
     3361 attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc ccctccaccc
     3421 cacagtgggg caagcttatt accctgttat ccctaaccgg tctaataccc aatctctcgt
     3481 cttatccaga tgttttatac gccaagcttc tgacctcttc tcttcctccc acagggcctc
     3541 gagacaattg catcgatggt accagtgaaa gtctgcaatg aattcgagtt ggcgcgcctg
     3601 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     3661 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     3721 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     3781 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     3841 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     3901 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     3961 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4021 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4081 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4141 tccccgtgag ctcccaggcg cgccaactcg gtaccgtatc gatgtcgacg ttaacgctag
     4201 tgatatcaac tttgtataga aaagttggct ccgaattcgc ccttattaat tggctccggt
     4261 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttgtg gggaggggtc
     4321 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4381 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4441 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     4501 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     4561 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     4621 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     4681 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     4741 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     4801 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     4861 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     4921 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     4981 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     5041 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     5101 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     5161 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     5221 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     5281 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     5341 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     5401 agtttttttc ttccatttca ggtggatccc aagggcgaat tcgacccaag tttgtacaaa
     5461 aaagcaggct ggatcccggt accctcgaca cctgctgaca ggtcgccacc atggtgtcct
     5521 ggggccggtt catctgcctg gtggtggtca caatggccac cctgagcctg gccagaccca
     5581 gcttcagcct ggtggaagat accaccctgg aaggcggcga gcagaagctg atcagcgaag
     5641 aggacctggg atccctggaa gccgaggcag ctgctaaaga ggctgcagcc aaggccctgg
     5701 aagaattcct gcctgcccct ggcagagaga aagagatcac cgccagcccc gactacctgg
     5761 aaatcgccat ctactgtatc ggcgtgttcc tgatcgcatg catggtggtc accgtgatcc
     5821 tgtgccggat gaagaacacc accaagaagc ccgacttcag cagccagccc gccgtgcaca
     5881 agctgaccaa gagaatcccc ctgcggagac aggtgtccgc cgagagcagc agcagcatga
     5941 acagcaacac ccccctggtc cgcatcacca ccagactgag cagcaccgcc gacaccccta
     6001 tgctggccgg cgtgtccgag tacgagctgc ccgaggaccc caagtgggag ttcccccggg
     6061 acaagcctgc aggcggcgga ggctccggtg gtggctctgg catggtgagc gagctgatta
     6121 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
     6181 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg
     6241 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
     6301 gcaaaacctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg
     6361 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     6421 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     6481 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga
     6541 ccctgtaccc cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg
     6601 gcgggggcca cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga
     6661 acctcaagat gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg
     6721 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
     6781 gcaaactggg gcacaaactt aatggcggag gtggatctgg cggaggtgga tcgggcggag
     6841 gtggatcaac cggtatgcag tctgtgctga ctcagccacc ctcagcgtct gggacccccg
     6901 ggcagagggt caccatctct tgttctggaa gcagctccaa catcggaagt aattatgtat
     6961 actggtacca gcagctccca ggaacggccc ccaaactcct catctatagg aataatcagc
     7021 ggccctcagg ggtccctgac cgattctctg gctccaagtc tggcacctca gcctccctgg
     7081 ccatcagtgg gctccggccc gaggatgagg ctgattatta ctgtgcagca tgggatgaca
     7141 gcctgtgtgt tgctctggta ttcggcggag ggaccaacta cccctacgac gtgcccgact
     7201 acgccaccac agaaaacctg tacttccagt ccggcatggc ccccccgacc gatgtcagcc
     7261 tgggggacga gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag
     7321 acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc
     7381 acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta
     7441 ccgatgccct tggaattgac gagtacggtg ggacgcgtat gaagctactg tcttctatcg
     7501 aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt
     7561 gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaaggtctc
     7621 cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat
     7681 ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat tctttacagg
     7741 atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa gatgccgtca
     7801 cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag catagaataa
     7861 gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg actgtataaa
     7921 agcttgcttc agtgcaggtg aattctacca cccagctttc ttgtacaaag tggtacgcgt
     7981 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     8041 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     8101 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     8161 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     8221 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     8281 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     8341 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     8401 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     8461 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
     8521 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8581 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8641 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8701 gtcgtgccag cggatcgaca gtactaagct tggtgaattc ccttatgtga gtgtaaaagg
     8761 caggcgagtt tgtcccttaa ttaataggga taacagggta atctcgaggt ttaaacgtcg
     8821 acgcggccgc tactagggac aggattggtg acagaaaagc cccatcctta ggcctcctcc
     8881 ttcctagtct cctgatattg ggtctaaccc ccacctcctg ttaggcagat tccttatctg
     8941 gtgacacacc cccatttcct ggagccatct ctctccttgc cagaacctct aaggtttgct
     9001 tacgatggag ccagagagga tcctgggagg gagagcttgg cagggggtgg gagggaaggg
     9061 ggggatgcgt gacctgcccg gttctcagtg gccaccctgc gctaccctct cccagaacct
     9121 gagctgctct gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc agcttcctta
     9181 cacttcccaa gaggagaagc agtttggaaa aacaaaatca gaataagttg gtcctgagtt
     9241 ctaactttgg ctcttcacct ttctagtccc caatttatat tgttcctccg tgcgtcagtt
     9301 ttacctgtga gataaggcca gtagccagcc ccgtcctggc agggctgtgg tgaggagggg
     9361 ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg
     9421 tggccgcctc tactcccttt ctctttctcc atccttcttt ccttaaagag tccccagtgc
     9481 tatctgggac atattcctcc gcccagagca gggtcccgct tccctaaggc cctgctctgg
     9541 gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc ctgtccccct
     9601 tcctcgtcca ccatctcatg cccctggctc tcctgcccct tccctacagg ggttcctggc
     9661 tctgctctcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     9721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9901 agctgcatta atgaatc
//
