LOCUS       40924_18796        4188 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pEX302, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Zaltsman A, Krichevsky A, Loyter A, Citovsky V.
  TITLE     Agrobacterium induces expression of a host F-box protein required 
            for tumorigenicity
  JOURNAL   Cell Host Microbe 7 (3), 197-209 (2010)
  PUBMED    20227663
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Zaltsman A, Krichevsky A, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2008) Biochemistry and Cell Biology, SUNYSB, NY 
            11794, USA
REFERENCE   3  (bases 1 to 4188)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4188)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe"; date: "2010"; volume: "7"; issue: "3"; pages: "197-209"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-OCT-2008) Biochemistry and Cell Biology, SUNYSB, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..87
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        94..112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     249..265
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(299..315)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(889..907)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(928..944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(952..968)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(976..1006)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1021..1042)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1114..2259)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2561..3352)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(join(3561..4188,1..2))
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
       61 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
      121 ggtaccttag aaacctctgg aggtggaacg ctctcttgga cgtgaacctt cgaaaatcat
      181 agacctcgcg gatttgggct tcggtgaagg aatcggcagt ggcacactaa gcaattgatt
      241 cagcaactcg aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
      301 tccactagtt ctagagcggc cgccaccgcg gtgcatgctc cttctttctc tcaatcggtc
      361 ggaacctact gtgataaaga gagagcctca atttttattg tattactttt gcaattaaca
      421 acctgtgatt caattgacct ggtgaaaatg cacgatgttg aagtagtcat tttccgaaac
      481 caaagcttga gtagtgtctt agcgccgtag atggtcaccc tcacgtttaa gcgccaagcg
      541 attccgattg ccacaggcgg aatgatataa gaaaatagct tatggtcgat cttatagagt
      601 aatattaatc agactatagc atgatttttc tcctaacgca catgctgcag gctgactgat
      661 tataaagaga atctttacat ttcgattctc gaataagata ccgaaccacg tcgaaacggt
      721 cgcattcctg ggggcagaac aggattaatg cgaaatcgta cgctctcatg agcgatttag
      781 gttttccggg cgcatttaaa ctaccccgtt ttcgcgaggt cagtcaattg atgcagcctg
      841 tgcacggtgc tggagatggc gatccgaacc gagctccagc ttttgttccc tttagtgagg
      901 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      961 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1021 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1081 cctgtcgtgc cagactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
     1141 gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg
     1201 ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag
     1261 cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg
     1321 cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac
     1381 gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac
     1441 gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc
     1501 catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt
     1561 gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat
     1621 aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg
     1681 cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt
     1741 ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt
     1801 gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg
     1861 catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac
     1921 ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt
     1981 gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc
     2041 cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat
     2101 cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg
     2161 catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag
     2221 ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc
     2281 cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt
     2341 gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt
     2401 ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata
     2461 cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc
     2521 ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta aaacaattca tccagtaaaa
     2581 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     2641 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     2701 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     2761 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     2821 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggggtgtct tcttcccagt
     2881 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
     2941 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     3001 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     3061 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaggt
     3121 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
     3181 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
     3241 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
     3301 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
     3361 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
     3421 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
     3481 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt
     3541 atgggtgatg ctgcaactcg agcgggccgg gagggttcga gaaggggggg cacccccctt
     3601 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
     3661 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
     3721 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
     3781 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
     3841 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
     3901 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
     3961 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
     4021 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
     4081 acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg
     4141 gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgag
//
