LOCUS       40924_18791        5791 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for bacterial expression of lacZ fusion proteins. For other 
            reading frames, use pEX1 or pEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Stanley KK, Luzio JP.
  TITLE     Construction of a new family of high efficiency bacterial expression
            vectors: identification of cDNA clones coding for human liver 
            proteins.
  JOURNAL   EMBO J. 1984;3:1429-34.
  PUBMED    6086324
REFERENCE   2  (bases 1 to 5791)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1984"; volume: "3"; pages: "1429-34"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             241..3198
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VSRLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPA
                     PEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYS
                     LTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENR
                     LAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEA
                     EVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLW
                     SAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHH
                     PLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG
                     MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSV
                     DPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCE
                     YAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTP
                     NDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWM
                     VALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHI
                     SAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDK
                     KQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD
                     AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQV
                     AERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQ
                     WRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE"
     terminator      3299..3347
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     misc_feature    3632..3772
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3958..4546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4720..5577)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5578..5682)
                     /label=AmpR promoter
ORIGIN
        1 cgcaagggat aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat
       61 ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga agcttgggat
      121 cgatccggag cttggctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
      181 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      241 gtttcccgac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      301 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
      361 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
      421 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
      481 accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
      541 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
      601 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
      661 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
      721 gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag
      781 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
      841 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
      901 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
      961 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     1021 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     1081 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     1141 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
     1201 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
     1261 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
     1321 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
     1381 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
     1441 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
     1501 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
     1561 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
     1621 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
     1681 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
     1741 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
     1801 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
     1861 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
     1921 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
     1981 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
     2041 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
     2101 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
     2161 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
     2221 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
     2281 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
     2341 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
     2401 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
     2461 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
     2521 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
     2581 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
     2641 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
     2701 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
     2761 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
     2821 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
     2881 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
     2941 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
     3001 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
     3061 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
     3121 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
     3181 ccgtcagtat cggcggaatt aattcccggg gatccgtcga cctgcagcca agcttgctga
     3241 ttgattgacc ggatcgatcc ggctctagaa ttaattcacc tcgaaagcaa gctgataaac
     3301 cgatacaatt aaaggctcct tttggagcct ttttttttgg agattttcaa cgtgaaaaaa
     3361 ttattattcg caattcaagc taattcacct agaaagcaag ctgataaacc gatacaatta
     3421 aaggctcctt ttggagcctt tttttttgga gattttcaac gtgaaaaaat tattattcgc
     3481 aattcaagct ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     3541 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     3601 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     3661 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     3721 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     3781 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3841 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3961 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4021 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4081 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4141 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4201 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4261 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4321 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4381 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4441 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4501 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4561 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4621 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4681 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4741 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4801 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4861 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4921 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4981 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5041 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5101 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5161 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5221 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5281 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5341 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5401 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5461 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5521 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5581 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5641 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5701 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5761 cacgaggccc tttcgtcttc aagaattaat t
//
