LOCUS       V012516                 9214 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V012516
VERSION     V012516
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..840)
                     /direction=LEFT
                     /label="ARS1"
                     /note="S. cerevisiae autonomously replicating sequence
                     ARS1/ARS416"
     terminator      complement(842..1089)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             2103..2552
                     /gene="ANB1"
                     /label="Eukaryotic translation initiation factor 5A-2"
                     /note="Eukaryotic translation initiation factor 5A-2 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P19211"
     promoter        3627..3847
                     /label="URA3 promoter"
     CDS             3848..4648
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      5577..6919
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     misc_feature    7061..7201
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7387..7975)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8149..9006)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9007..9111)
                     /label="AmpR promoter"
ORIGIN
        1 ttccacatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta
       61 agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca
      121 tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg
      181 gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc
      241 gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt
      301 tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta
      361 catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca
      421 agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca
      481 ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca
      541 aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag
      601 atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa
      661 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga
      721 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa
      781 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct
      841 tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt
      901 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc
      961 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc
     1021 cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta
     1081 attacatgat atcgacaaag gaaaaggggg acggatcccc gggaattcag ttattattaa
     1141 tttagtgtgt gtatttgtgt ttgcgtgtct atagaagtat agtaatttat gctacaaagg
     1201 acctaatgta taaggaaaga atatttagag aaaagaagaa aacaagagtt ttatatacat
     1261 acagagcaca tgcatgccat atgatcatgt gtcgtcgcac acatatatat atgcctgtat
     1321 gtgtcagcac taaagttgcc tggccatcca cgctatatac acgcctggcg gatctgctcg
     1381 aggattgcct acgcgtgggc ttgatccacc aaccaacgct cgccaaatga gctggcgctt
     1441 ggtcttcggt catcgtccgt aaaccccggc caaagagacc ggaaagatcg gtgaaaacat
     1501 cttgatcttg ctcccgggaa ttttagattc aggtaggaaa ttgattacat aaatactgtt
     1561 accctgaatc atattcgacg atgtcgtctc acacggaaat ataattcatt tcttggtttt
     1621 ccaaaaaaat tttcattttt tttcactttt ttgtttcgtc ctcctttttt ttttttttta
     1681 ttttttttcc tgtgttcacc tttttttttt ttttttttca gtttacaact ttctgcattc
     1741 ttttctgtgt tttttttttt ttcgtttttc catttgttcg ttcgttgcct gtttttttgc
     1801 cctattgttc tcgagcctaa aaattttttc ctttcctgct ttcctttctt cgttcaaagt
     1861 ttcctattcc attgttctct tcggtaaact cattgttgct cggaactcag atatattcag
     1921 gtcaatttac tgtacttcaa ttgacttttt tcttgaaatt tcaacttgcc tttcaacttg
     1981 ttcttctttt ttaattctta ttctacactt tagttccctt accttgttcc taattattgt
     2041 ctgcaaaaga aaacatacac ctatttcatt cacacactaa aacatgtctg acgaagaaca
     2101 caacctttga aaatgctgac gctggtgcct ccgccaccta cccaatgcaa tgttctgcct
     2161 tgagaaagaa tggtttcgtt gtcatcaaag gtagaccatg taagattgtc gacatgtcca
     2221 cttccaagac cggtaagcac ggtcacgcca aggtccattt ggttaccctt gatatcttca
     2281 ctggtaagaa gttggaagat ttgtctccat ccactcacaa cttggaagtt ccatttgtca
     2341 agagaagcga ataccaattg ttggatattg atgatggcta cttgtccttg atgaccatga
     2401 acggtgaaac caaagacgat gtcaacgcac cagaaggtag gttgggtgac agcatgcaag
     2461 ctgctttcga tgaaggtaag gatttgatgg tcactatcat ttctgccatg ggtgaagaag
     2521 ctgccatctc cttcaaggaa gctccaagat cctctacgcc ggacgcatcg tggccggcat
     2581 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     2641 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     2701 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     2761 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     2821 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     2881 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     2941 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     3001 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     3061 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     3121 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     3181 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     3241 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
     3301 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     3361 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
     3421 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc
     3481 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
     3541 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg
     3601 gggggggggg gggggggggg gagcttttca attcaattca tcattttttt tttattcttt
     3661 tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga
     3721 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
     3781 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
     3841 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
     3901 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
     3961 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
     4021 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
     4081 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
     4141 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
     4201 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
     4261 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
     4321 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
     4381 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
     4441 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
     4501 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
     4561 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
     4621 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
     4681 actaaactca caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg
     4741 tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga aagaaaaagc tccccccccc
     4801 cccccccccc ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     4861 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     4921 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     4981 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     5041 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     5101 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     5161 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     5221 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
     5281 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     5341 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     5401 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc aggactaatg
     5461 ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg tatactcatc
     5521 agataacagc aatacctgat cactacttcg cactagtttc tcggtactat gcatatgatc
     5581 caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc
     5641 atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga
     5701 taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac
     5761 gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac
     5821 acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt
     5881 cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag
     5941 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca
     6001 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca
     6061 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc
     6121 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg
     6181 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga
     6241 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga
     6301 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca
     6361 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa
     6421 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt
     6481 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca
     6541 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat
     6601 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag
     6661 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc
     6721 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag
     6781 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc
     6841 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat
     6901 gaagcacaga tgcttcgttc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6961 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7021 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     7081 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     7141 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     7201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7381 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7441 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7501 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7561 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7621 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7681 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7741 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7801 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7861 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7921 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7981 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8041 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8461 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8761 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9121 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     9181 taggcgtatc acgaggccct ttcgtcttca agaa
//
