LOCUS       V013759                 5362 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013759
VERSION     V013759
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(318..1328)
                     /gene="pheS"
                     /label="Phenylalanine--tRNA ligase alpha subunit"
                     /note="Phenylalanine--tRNA ligase alpha subunit from
                     Burkholderia pseudomallei (strain 1710b). Accession#:
                     Q3JT08"
     oriT            complement(1441..1550)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      complement(1993..2020)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2112..2198)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     2548..2564
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(2568..2624)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2634..2650)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2658..2674)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2682..2712)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2727..2748)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3036..3624)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3901..5088)
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        complement(5163..5267)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataatcg gcattttctt ttgcgttttt atttgttaac tgttaattgt ccttgttcaa
      241 ggatgctgtc tttgacaaca gatgttttct tgcctttgat gttcagcagg aagctaggcg
      301 caaacgttgg atctttaggc gaactggcgg aggaaccgga ggtcattttc gaaaaagagc
      361 cggaggtcct ggacaccata ccggagcatg gtaaggcgtt ccaacccgga accgaagccg
      421 aagccaatgt aacgctccgg gtcgaggccc atattccgaa tgaccgtggg gtggacctga
      481 ccgctgccgc tgatttccaa ccacttacca gcattcttcc catgttcaaa catcatatca
      541 atttccgcgc tgggttccgt gaaggggaag tagctcgggc ggaaacggac caagatgtca
      601 tctcgttcga aaaatttctt gagaaagtcg gtgtaggcac ccttgagatc ggcaaacgac
      661 acgttttcat caatccagag cccctccacc tgattaaaca tggggctatg ggtggcgtcc
      721 gaatcgaccc gataggtccg accgggagca atcaccttaa tcgggggtcg attcatgcgg
      781 gcgtaccgca cctgcatggg cgaggtatgg gtccggagga ggaggggccg cccatcggcg
      841 tccttaccat ccacatagaa ggtgtcctgc atggaccgtg cggggtggtt ttcgggcgag
      901 ttgagcgccg tgaaattata ccaatccgtc tcaatttccg gtccatccgc gacatcgaag
      961 ccaatgctac ggaaaatctg ctccacacgt tcccaggttc gcatgacggg gtgcagcgag
     1021 ccggcgcccg ccccgcgacc ggggagggtc acatcaatgg cttcagccgc caggcgctga
     1081 ttgagaaggg catccgccaa ggcctgccgc cgggcattca aggccgcctc cacctgctgc
     1141 ttggccacgt taatccgggc accctcggtc ttccgggttt ccgggtccag cttacccagc
     1201 cccttcaaga gttccgtgag ggcaccgctc ttgcccagga agcgggcctt ttcattttcc
     1261 aaggtcgtaa tgtcggccgc gccctcaaag ctctgctggg catccgccac gatctgatcg
     1321 aggtccatat gcttgtcctt tcgaagctcg atccgattgt cagacccggc atgatattcc
     1381 gaaaatccca agcgagtcaa cagctagtgc ttgccctcag tactgttacg ccggcggtag
     1441 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
     1501 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
     1561 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     1621 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
     1681 aaaagcgctg cttccctgct gttttgtgga atatctaccg actggaaaca ggcaaatgca
     1741 ggaaattact gaactgaggg gacaggcgag agacgatgcc aaagagctac accgacgagc
     1801 tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg gcgtgatgag gctgcggttg
     1861 cgttcctggc ggtgagggcg gatgtcgata tgcggtgtga aataccgcac agatgcgtaa
     1921 ggagaaaata ccgcatcagg cgcatatttg aatgtattta gaaaaataaa caaaagagtt
     1981 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca
     2041 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc
     2101 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     2161 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     2221 cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat
     2281 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct
     2341 tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc
     2401 aagctcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2461 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     2521 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc
     2581 tgcaggtcga ctctagagga tccccgggta ccgagctcga attcgtaatc atggtcatag
     2641 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2701 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2761 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2821 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2881 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2941 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3001 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3061 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3541 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3601 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3661 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3721 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3781 acttggtctg acaatcgatg caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     3841 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     3901 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     3961 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     4021 cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg
     4081 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     4141 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     4201 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     4261 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     4321 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     4381 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     4441 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     4501 ggctctcaag ggcatcggtc ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc
     4561 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gtaaggagat
     4621 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     4681 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     4741 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agagaatcca
     4801 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     4861 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     4921 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     4981 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     5041 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     5101 actgcgttag caatttaact gtgataaact accgcattaa agctaatcgg ttgaatactc
     5161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5281 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     5341 cgtatcacga ggccctttcg tc
//
