LOCUS       V006440                 4822 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006440
VERSION     V006440
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.
  TITLE     Genetic tools for allelic replacement in Burkholderia species
  JOURNAL   Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008)
   PUBMED   18502918
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Barrett AR, Kang Y, Vukovich JM, Son MS, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2007) Microbiology, University of Hawaii at Manoa,
            2538 McCarthy Mall Snyder Hall Rm 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 4822)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4822)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "14";
            pages: "4498-4508"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-DEC-2007) Microbiology, University of Hawaii at Manoa, 2538
            McCarthy Mall Snyder Hall Rm 310, Honolulu, HI 96822, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(318..1328)
                     /gene="pheS"
                     /label="Phenylalanine--tRNA ligase alpha subunit"
                     /note="Phenylalanine--tRNA ligase alpha subunit from
                     Burkholderia pseudomallei (strain 1710b). Accession#:
                     Q3JT08"
     regulatory      1364..1405
                     /label="PS12"
                     /note="PS12"
                     /regulatory_class="promoter"
     oriT            complement(1441..1550)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      complement(1971..1998)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2090..2176)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     2526..2542
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(2546..2602)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2612..2628)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2636..2652)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2660..2690)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2705..2726)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3014..3602)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3858..4388)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(4577..4605)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        complement(4623..4727)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataatcg gcattttctt ttgcgttttt atttgttaac tgttaattgt ccttgttcaa
      241 ggatgctgtc tttgacaaca gatgttttct tgcctttgat gttcagcagg aagctaggcg
      301 caaacgttgg atctttaggc gaactggcgg aggaaccgga ggtcattttc gaaaaagagc
      361 cggaggtcct ggacaccata ccggagcatg gtaaggcgtt ccaacccgga accgaagccg
      421 aagccaatgt aacgctccgg gtcgaggccc atattccgaa tgaccgtggg gtggacctga
      481 ccgctgccgc tgatttccaa ccacttacca gcattcttcc catgttcaaa catcatatca
      541 atttccgcgc tgggttccgt gaaggggaag tagctcgggc ggaaacggac caagatgtca
      601 tctcgttcga aaaatttctt gagaaagtcg gtgtaggcac ccttgagatc ggcaaacgac
      661 acgttttcat caatccagag cccctccacc tgattaaaca tggggctatg ggtggcgtcc
      721 gaatcgaccc gataggtccg accgggagca atcaccttaa tcgggggtcg attcatgcgg
      781 gcgtaccgca cctgcatggg cgaggtatgg gtccggagga ggaggggccg cccatcggcg
      841 tccttaccat ccacatagaa ggtgtcctgc atggaccgtg cggggtggtt ttcgggcgag
      901 ttgagcgccg tgaaattata ccaatccgtc tcaatttccg gtccatccgc gacatcgaag
      961 ccaatgctac ggaaaatctg ctccacacgt tcccaggttc gcatgacggg gtgcagcgag
     1021 ccggcgcccg ccccgcgacc ggggagggtc acatcaatgg cttcagccgc caggcgctga
     1081 ttgagaaggg catccgccaa ggcctgccgc cgggcattca aggccgcctc cacctgctgc
     1141 ttggccacgt taatccgggc accctcggtc ttccgggttt ccgggtccag cttacccagc
     1201 cccttcaaga gttccgtgag ggcaccgctc ttgcccagga agcgggcctt ttcattttcc
     1261 aaggtcgtaa tgtcggccgc gccctcaaag ctctgctggg catccgccac gatctgatcg
     1321 aggtccatat gcttgtcctt tcgaagctcg atccgattgt cagacccggc atgatattcc
     1381 gaaaatccca agcgagtcaa cagctagtgc ttgccctcag tactgttacg ccggcggtag
     1441 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
     1501 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
     1561 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     1621 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
     1681 aaaagcgctg cttccctgct gttttgtgga atatctaccg actggaaaca ggcaaatgca
     1741 ggaaattact gaactgaggg gacaggcgag agacgatgcc aaagagctac accgacgagc
     1801 tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg gcgtgatgag gctgcggttg
     1861 cgttcctggc ggtgagggcg gatgtcgata tgcgtaagga gaaaataccg catcaggcgc
     1921 atatttgaat gtatttagaa aaataaacaa aaagagtttg tagaaacgca aaaaggccat
     1981 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     2041 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     2101 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     2161 cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
     2221 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
     2281 ctactgccgc caggcaaatt ctgttttatc agaccgcttc tgcgttctga tttaatctgt
     2341 atcaggctga aaatcttctc tcatccgcca aaacagccaa gctcgccatt cgccattcag
     2401 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     2461 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     2521 acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcgact ctagaggatc
     2581 cccgggtacc gagctcgaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2641 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2701 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2761 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2821 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2881 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2941 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3001 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3061 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3121 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3181 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3241 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3361 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3421 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3481 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3661 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3721 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac aatcgatgcg
     3781 aattggccgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg
     3841 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
     3901 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
     3961 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
     4021 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta
     4081 cgcggctgct caaacctggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg
     4141 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag
     4201 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga
     4261 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata
     4321 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg
     4381 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     4441 cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc
     4501 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     4561 acgctacttg cattacagtt tacgaaccga acaggcttat gtcaattcgg ttgaatactc
     4621 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4681 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4741 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     4801 cgtatcacga ggccctttcg tc
//
