LOCUS       40924_18726        5783 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for bacterial expression of lacZ fusion proteins. For other 
            reading frames, use pEX2 or pEX3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Stanley KK, Luzio JP.
  TITLE     Construction of a new family of high efficiency bacterial expression
            vectors: identification of cDNA clones coding for human liver 
            proteins.
  JOURNAL   EMBO J. 1984;3:1429-34.
  PUBMED    6086324
REFERENCE   2  (bases 1 to 5783)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1984"; volume: "3"; pages: "1429-34"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             241..3195
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VSRLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPA
                     PEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYS
                     LTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENR
                     LAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEA
                     EVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLW
                     SAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHH
                     PLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG
                     MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSV
                     DPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCE
                     YAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTP
                     NDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWM
                     VALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHI
                     SAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDK
                     KQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD
                     AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQV
                     AERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQ
                     WRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSA"
     terminator      3291..3339
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     misc_feature    3624..3764
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3950..4538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4712..5569)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5570..5674)
                     /label=AmpR promoter
ORIGIN
        1 cgcaagggat aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat
       61 ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga agcttgggat
      121 cgatccggag cttggctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
      181 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      241 gtttcccgac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      301 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
      361 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
      421 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
      481 accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
      541 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
      601 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
      661 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
      721 gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag
      781 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
      841 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
      901 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
      961 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     1021 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     1081 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     1141 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
     1201 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
     1261 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
     1321 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
     1381 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
     1441 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
     1501 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
     1561 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
     1621 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
     1681 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
     1741 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
     1801 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
     1861 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
     1921 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
     1981 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
     2041 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
     2101 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
     2161 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
     2221 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
     2281 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
     2341 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
     2401 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
     2461 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
     2521 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
     2581 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
     2641 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
     2701 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
     2761 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
     2821 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
     2881 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
     2941 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
     3001 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
     3061 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
     3121 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
     3181 ccgtcagtat cggcggcccg gggatccgtc gacctgcagc caagcttgct gattgattga
     3241 ccggatcgat ccggctctag aattaattca cctcgaaagc aagctgataa accgatacaa
     3301 ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa aattattatt
     3361 cgcaattcaa gctaattcac ctagaaagca agctgataaa ccgatacaat taaaggctcc
     3421 ttttggagcc tttttttttg gagattttca acgtgaaaaa attattattc gcaattcaag
     3481 ctctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3541 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3601 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     3661 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     3721 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     3781 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3841 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3901 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3961 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4021 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4081 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4141 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4201 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4261 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4321 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4381 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4441 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4501 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4561 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4621 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4681 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4741 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4801 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4861 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4921 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4981 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5041 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5101 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5161 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5221 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5281 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5341 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5401 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5461 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5521 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5581 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5641 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5701 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     5761 cctttcgtct tcaagaatta att
//
