LOCUS       40924_18716        7511 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pEX0, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7511)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7511)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..840)
                     /direction=LEFT
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     terminator      complement(842..1089)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        1924..2144
                     /label=URA3 promoter
     CDS             2145..2945
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      3874..5216
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     misc_feature    5358..5498
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5684..6272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6446..7303)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7304..7408)
                     /label=AmpR promoter
ORIGIN
        1 ttccacatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta
       61 agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca
      121 tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg
      181 gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc
      241 gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt
      301 tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta
      361 catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca
      421 agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca
      481 ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca
      541 aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag
      601 atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa
      661 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga
      721 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa
      781 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct
      841 tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt
      901 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc
      961 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc
     1021 cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta
     1081 attacatgat atcgacaaag gaaaaggggg acggatcccg tcgaccgatg cccttgagag
     1141 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     1201 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     1261 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     1321 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     1381 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     1441 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     1501 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     1561 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca
     1621 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     1681 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     1741 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     1801 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     1861 gtccgccatc tccagcagcc gcacgcggcg catctcgggg gggggggggg ggggggggag
     1921 cttttcaatt caattcatca tttttttttt attctttttt ttgatttcgg tttctttgaa
     1981 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
     2041 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
     2101 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat
     2161 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc
     2221 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg
     2281 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga
     2341 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt
     2401 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact
     2461 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg
     2521 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag
     2581 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta
     2641 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
     2701 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
     2761 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta
     2821 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
     2881 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
     2941 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
     3001 caatttaatt atatcagtta ttacccggga atctcggtcg taatgatttt tataatgacg
     3061 aaaaaaaaaa aattggaaag aaaaagctcc cccccccccc cccccccctc gggcagcgtt
     3121 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     3181 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     3241 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     3301 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     3361 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     3421 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     3481 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     3541 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     3601 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     3661 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     3721 cacgaccacg ctgatgagct ttaccgcagg actaatgttg tgggaaattg gagcgataag
     3781 cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat acctgatcac
     3841 tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga aatgatagca
     3901 ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt
     3961 gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac
     4021 tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact
     4081 atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga
     4141 acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac
     4201 gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     4261 ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact
     4321 gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc
     4381 tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt
     4441 gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta
     4501 gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta
     4561 aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga
     4621 ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg
     4681 gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt
     4741 aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa
     4801 agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt
     4861 atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc
     4921 tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc
     4981 tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag
     5041 cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat
     5101 agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa
     5161 attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgttctgc
     5221 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     5281 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     5341 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     5401 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     5461 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     5521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6121 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6721 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     6781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6961 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7021 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     7081 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7141 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7201 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7261 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7321 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7381 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     7441 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     7501 gtcttcaaga a
//
